Production of indole-3-acetic acid by Bacillus circulans E9 in a low-cost medium in a bioreactor

Sarmiento-López, Luis G.; López-Meyer, Melina; Maldonado-Mendoza, Ignacio E.; Quiroz-Figueroa, Francisco Roberto; Sepúlveda-Jiménez, Gabriela; Rodrı́guez-Monroy, Mario

doi:10.1016/j.jbiosc.2022.03.007

Cited by 18 publications

(7 citation statements)

References 52 publications

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“…Colonization of plant roots by two or more AMF species has previously been reported to provide a spectrum of advantages, and has more benefits to host plants than colonization by one species (Sharma and Kapoor, 2023). The strain N. circulans E9 has been found to promote plant growth (Sarmiento-López et al, 2022), which is associated with production of IAA and promotion of growth through stimulation of cell division and increased nutrient and water uptake, resulting in increased crop yield and quality (Sarmiento-López et al, 2022). In the present study, increases in the growth parameters of R. solani-infected eggplants were detected after individual treatments with each of the two bio-inoculants.…”

Section: Discussionmentioning

confidence: 99%

Combined interaction between the diazotrophic Niallia circulans strain YRNF1 and arbuscular mycorrhizal fungi in promoting growth of eggplant and mitigating root rot stress caused by Rhizoctonia solani

RASHAD,

BOUQELLAH,

HAFEZ

et al. 2024

Phytopathol. Mediterr.

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Rhizoctonia root rot of eggplant, caused by Rhizoctonia solani, is an economically important disease. Niallia circulans YRNF1 and arbuscular mycorrhizal fungi (AMF) were assessed for their biocontrol and biofertilizing effects against R. solani, as potential replacements for synthetic fungicides and fertilizers. The diazotrophic N. circulans YRNF1, isolated from soil, reduced in vitro growth of R. solani by 42%. GC-MS analysis of culture filtrate of N. circulans YRNF1 detected bioactive compounds, including butyric acid (85%) and ethylene glycol (8%). In greenhouse experiments, combined application of N. circulans YRNF1 and AMF reduced the severity of eggplant root rot by 26%. This combined treatment triggered the transcriptional expression of five resistance genes (JERF3, PAL1, C3H, CHI2, and HQT) in the treated eggplants. Biochemical analyses of the infected eggplant roots treated with the combined bio-inoculants showed enhancement of the phenol content (+188%), and increased antioxidant enzyme activity, mainly of POD (+104%) and PPO (+72%). Combined application of N. circulans YRNF1 and AMF also promoted eggplant growth and improved the total NPK concentrations in treated plant leaves. Inoculation of eggplant with N. circulans YRNF1 in the presence of AMR increased the mycorrhization level. This is the first report of N. circulans and AMF as potential agents for biological control of Rhizoctonia root rot and growth promotion of eggplant.

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Section: Discussionmentioning

confidence: 99%

Combined interaction between the diazotrophic Niallia circulans strain YRNF1 and arbuscular mycorrhizal fungi in promoting growth of eggplant and mitigating root rot stress caused by Rhizoctonia solani

RASHAD,

BOUQELLAH,

HAFEZ

et al. 2024

Phytopathol. Mediterr.

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“…The amount of indole-3-acetic acid synthesized by the consortiums was determined according to the method developed by Sarmiento-Lopez et al [46]. According to this method, 0.1% L-tryptophan and 5% of the bacterial consortium were added to the beef extract broth.…”

Section: Methodsmentioning

confidence: 99%

Microorganisms for Bioremediation of Soils Contaminated with Heavy Metals

et al. 2023

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Heavy-metal contaminants are one of the most relevant problems of contemporary agriculture. High toxicity and the ability to accumulate in soils and crops pose a serious threat to food security. To solve this problem, it is necessary to accelerate the pace of restoration of disturbed agricultural lands. Bioremediation is an effective treatment for agricultural soil pollution. It relies on the ability of microorganisms to remove pollutants. The purpose of this study is to create a consortium based on microorganisms isolated from technogenic sites for further development in the field of soil restoration in agriculture. In the study, promising strains that can remove heavy metals from experimental media were selected: Pantoea sp., Achromobacter denitrificans, Klebsiella oxytoca, Rhizobium radiobacter, and Pseudomonas fluorescens. On their basis, consortiums were compiled, which were investigated for the ability to remove heavy metals from nutrient media, as well as to produce phytohormones. The most effective was Consortium D, which included Achromobacter denitrificans, Klebsiella oxytoca, and Rhizobium radiobacter in a ratio of 1:1:2, respectively. The ability of this consortium to produce indole-3-acetic acid and indole-3-butyric acid was 18.03 μg/L and 2.02 μg/L, respectively; the absorption capacity for heavy metals from the experimental media was Cd (56.39 mg/L), Hg (58.03 mg/L), As (61.17 mg/L), Pb (91.13 mg/L), and Ni (98.22 mg/L). Consortium D has also been found to be effective in conditions of mixed heavy-metal contamination. Due to the fact that the further use of the consortium will be focused on the soil of agricultural land cleanup, its ability to intensify the process of phytoremediation has been studied. The combined use of Trifolium pratense L. and the developed consortium ensured the removal of about 32% Pb, 15% As, 13% Hg, 31% Ni, and 25% Cd from the soil. Further research will be aimed at developing a biological product to improve the efficiency of remediation of lands withdrawn from agricultural use.

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“…indole-3-acetic acid (IAA) dapat dihasilkan oleh bakteri Bacillus sp. dimana IAA merupakan hormon jenis auksin untuk proses pemanjangan sel (Sarmiento-López et al, 2022). Ditunjang dengan ketersediaan air dari akar adventif maupun akar utama, proses pemanjangan sel akan berlangsung dengan maksimal pada vanili dengan media yang diperkaya BPF maupun BPK.…”

Section: Panjang Sulurunclassified

Pengaruh Pemberian IAA pada Pembibitan Setek Vanili (Vanila planifolia) yang diperkaya Bakteri Pelarut Fosfat dan Bakteri Pelarut Kalium

Kusbianto

Emiyati

Setiawati

et al. 2022

J. Agro Ind. Perkeb.

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Propagation of vanilla plants is generally using vegetative methods like cuttings. The use of external growth regulators can support the growth of nurseries. Phosphate solubilising bacteria and potassium solubilising bacteria as plant growth promoting rhizobacteria are producing microbial IAA. This study used auxin in synthetic IAA combined with IAA microbes from phosphate solubilising bacteria and potassium solubilising bacteria. This study aimed to determine the effectiveness of each microbe at each dose of synthetic IAA that was applied. The study was conducted from November 2021-April 2022 in the Biological Soil Laboratory and Greenhouse University of Jember. The research uses a randomised block design with three replications with the factorial arrangement. The first factor was organic matter which was enriched with bacterial isolate (B0 = control, B1 = BPF isolate, and B2 = BPK isolate). The second factor was concentration of IAA (A0 = 0 mg.l-1, A1 = 100 mg.l-1, and A2 = 200 mg.l-1). The data were analysed by ANOVA and further tested with Duncan Multiple Range Test (DMRT) with a 95% confidence level. The results showed that treatment of synthetic IAA affects the parameters of length and volume of adventitious roots, which are effective in helping to supply nutrients with the best treatment at a concentration of 200 ppm. Meanwhile, enrichment of media with isolates of phosphate solubilising bacteria and potassium solubilising bacteria gave a better effect than control on the observation of tendril length, number of leaves, number of adventitious roots and taproots, length of adventitious roots and taproots, the volume of adventitious roots and taproot, and fresh weight of vanilla seedlings.

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Production of indole-3-acetic acid by Bacillus circulans E9 in a low-cost medium in a bioreactor

Cited by 18 publications

References 52 publications

Combined interaction between the diazotrophic Niallia circulans strain YRNF1 and arbuscular mycorrhizal fungi in promoting growth of eggplant and mitigating root rot stress caused by Rhizoctonia solani

Combined interaction between the diazotrophic Niallia circulans strain YRNF1 and arbuscular mycorrhizal fungi in promoting growth of eggplant and mitigating root rot stress caused by Rhizoctonia solani

Microorganisms for Bioremediation of Soils Contaminated with Heavy Metals

Pengaruh Pemberian IAA pada Pembibitan Setek Vanili (Vanila planifolia) yang diperkaya Bakteri Pelarut Fosfat dan Bakteri Pelarut Kalium

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