Production of insect resistant potato by genetic transformation with a δ-endotoxin gene from Bacillus thuringiensis var. kurstaki

Cheng, Junjun; Bolyard, Mark G.; Saxena, R. C.; Sticklen, Mariam B.

doi:10.1016/0168-9452(92)90027-j

Cited by 41 publications

(13 citation statements)

References 15 publications

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“…Bt ICPs have been used in conventional insecticidal sprays since the 1950s [30]. Genes encoding some of the Bt ICPs have recently been cloned and transferred to crop plants to reduce the need for spraying and to increase the activity and persistence ofBt ICPs [5,12,22,28,37,45]. Unfortunately, some insect species have developed resistance to Bt ICPs in laboratory selection experiments [25,36,38,45], and one insect, the diamondback moth (DBM), Plutella xylostella, has evolved resistance to Bt sprays in the field [ 15,21,31,33,38,40].…”

Section: Introductionmentioning

confidence: 99%

Transgenic broccoli expressing aBacillus thuringiensis insecticidal crystal protein: Implications for pest resistance management strategies

et al. 1995

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We used Agrobacterium tumefaciens to transform flowering stalk explants of five genotypes of broccoli with a construct containing the neomycin phosphotransferase gene and a Bacillus thuringiensis (Bt) gene [CryIA(c) type] optimized for plant expression. Overall transformation efficiency was 6.4~o; 181 kanamycin-resistant plants were recovered. Of the 162 kanamycin-resistant plants tested, 112 (69~o) caused 100 ~o morality of 1 st-instar larvae of a Bt-susceptible diamondback moth strain. Southern blots of some resistant transformants confirmed presence of the Bt gene. Selected plants that gave 100~ mortality of susceptible larvae allowed survival of a strain of diamondback moth that had evolved resistance to Bt in the field. F 1 hybrids between resistant and susceptible insects did not survive. Analysis of progeny from 26 resistant transgenic lines showed 16 that gave segregation ratios consistent with a single T-DNA integration. Southern analysis was used to verify those plants possessing a single T-DNA integration. Because these transgenic plants kill susceptible larvae and F 1 larvae, but serve as a suitable host for resistant ones, they provide an excellent model for tests of Bt resistance management strategies.

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Section: Introductionmentioning

confidence: 99%

Transgenic broccoli expressing aBacillus thuringiensis insecticidal crystal protein: Implications for pest resistance management strategies

et al. 1995

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“…toxin were only resistant to those insect species that were the most susceptible to the toxin (Fischhoff et al, 1987;Delanney et al, 1989). The problem appeared to be at the level of mRNA accumulation, because the plants that accumulated detectable B.t.-toxin mRNA were also the most insect resistant (Barton et al, 1987;Vaeck et al, 1987;Cheng et al, 1992;Dandekar et al, 1994).…”

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confidence: 99%

Premature Polyadenylation at Multiple Sites within aBacillus thuringiensis Toxin Gene-Coding Region1

et al. 1998

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-toxin-coding region corresponding to the short transcripts. A fourth polyadenylation site was identified using a chimeric gene. These results demonstrate for the first time to our knowledge that premature polyadenylation can limit the expression of a foreign gene in plants. Moreover, this work emphasizes that further study of the fundamental principles governing polyadenylation in plants will have basic as well as applied significance.

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“…Expression of only the NH2-terminal fragment of a &endotoxin gene produced plants with insecticidal activity. Likewise a native crylA(c)-Nptll fusion in potato showed little insecticidal activity [10], indicating a low level of expression [14]. An active crylIIA gene starting at amino acid 48 was introduced into tomato and potato plants [58].…”

Section: Synthetic Genesmentioning

confidence: 99%

Optimizing expression of transgenes with an emphasis on post-transcriptional events

1996

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Introducing a foreign gene into a new plant host does not always result in a high level of expression of the incoming gene. Numerous promoters have been used to express foreign genes in different plant tissues, but there are sometime various features of the new gene which are deleterious to expression in the new host. There are a number of post-transcriptional steps in the expression of a gene and sometimes sequences present in a particular coding region can resemble the signals which initiate these processing steps. When aberrantly carried out, these steps diminish the level of expression. By removing such fortuitous signals, one can dramatically increase expression of a transgene in plants. Ensuring proper protein folding and/or targeting the protein product to a particular cellular compartment can also be used to increase the level of protein obtained. The various methods used to optimize expression of a foreign gene in plants by concentrating on post-transcriptional events are discussed.

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Production of insect resistant potato by genetic transformation with a δ-endotoxin gene from Bacillus thuringiensis var. kurstaki

Cited by 41 publications

References 15 publications

Transgenic broccoli expressing aBacillus thuringiensis insecticidal crystal protein: Implications for pest resistance management strategies

Transgenic broccoli expressing aBacillus thuringiensis insecticidal crystal protein: Implications for pest resistance management strategies

Premature Polyadenylation at Multiple Sites within aBacillus thuringiensis Toxin Gene-Coding Region1

Optimizing expression of transgenes with an emphasis on post-transcriptional events

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