Production of keratinizing cysts within mandibles of rats with autogenous gingival epithelial grafts

IntroductionOdontogenic cysts in human beings are not uncommon. There are many types displaying various biologic behaviors, but little is known about their histopathogenesis. It is generally considered that each of the odontogenic cysts is derived from one of the following epithelial sources: 1) enamel organ, 2) reduced enamel epithelium of a tooth crown, 3) epithelial rests of Malassez and remnants of the sheath of Hertwig, 4) remnants of the dental lamina, or 5) basal cells of oral mucosa (1, 2). However, the exact relationship between these epithelia and various types of cysts is unknown, and experimental studies in this field have been limited. In order to investigate whether enamel organ is one of the sources of odontogenic cysts, and which type of odontogenic cyst is closely connected with it, we performed the following animal experiment. Materials and MethodsC3H/HeN mice were used. They were given mixing solid food and sterile drinking water . Two-month-old mice of both sexes mated at 9 o'clock at night and before 9 o'clock the next morning . The mice which had vaginal plugs were recognized as becoming pregnant after 0.5 day.And on day 17.5, the tooth germs of the embryos were in the bell stage , in which a specialization of cells of the enamel organ itself occur, resulting in the establishment of four distinct epithelial layers: external enamel epithelium, stellate reticulum, stratum intermedium, and internal enamel epithelium. The 17.5-day embryos were removed from pregnant females and placed in 2% bovine serum in Hanks solution. Under a dissecting microscope, the first mandibular molar tooth germs of these embryos were dissected from the surrounding connective tissues, dental lamina and oral epithelium . The tooth germs which were just in the bell stage, had only components of enamel organ, dental papilla and dental follicle, and not other tissues, such as dental lamina and oral epithelium (Fig. 1). Three-month-old syngenic male mice were anaesthetized by the intraperitioneal injection of pentobarbital sodium ( Sommonpentyl ) , and 32 dental germs were transplanted under the left renal subcapsular spaces of the mice at the rate of one graft per capita. The grafts were harvested 1, 2, 3, 4 weeks and 2, 3, 4, 5 months after transplantation. For histological examination, the grafts were fixed in 10% neutral buffered formalin and demineralized in 5% formic acid at 4 for 12 hours. Routinely the paraffin-embedded specimens were serially sectioned 4 µm thick and stained with hematoxylin-eosin.For determining the relationship between duration of transplantation and formation of experimental odontogenic cysts, pearson chi-squared test (χ 2 test) was applied. Results 1) Histopathological Findings(1) One-week group At 1 week after transplantation, small amounts of dentin and enamel appeared in the bell stage , tooth germs and stellate cells of enamel organs were still abundant. External enamel epithelium proliferated partially and the serial sections showed that these proliferations were sticking out of the ename...

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“…and Soskolne et al . (10,11). The histology of the cysts in these experiment was the similar to those seen in ours.…”

Section: Discussionsupporting

confidence: 82%

“…Formation of experimental odontogenic cysts by transplantation without any specific pretreatment has been reported by several authors (11)(12)(13)(14)(15)(16). Maeda et al .…”

Section: Discussionmentioning

confidence: 83%

Different Histogenesis of Experimental Odontogenic Cysts by the Renal Subcapsular Transplantation of Tooth Germs of Mice.

Xiao

Liu

et al. 2000

Oral Med Pathol

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“…Initial hyperplasia and cystic breakdown has been observed in other transplantation cysts (Bartlett et al 1973;Riviere & Sabet 1973), in radicular cysts forming in apical granulomas and in oral epithelium transplanted subcutaneously ( Van Scott & Reinertsen 1961) and intraosseously (Soskolne et al 1976). Thus there seems to be a similar mechanism of initiation of cyst formation in various sites under different circumstances.…”

Section: Discussionmentioning

confidence: 82%

A histological study of odontogenic cysts formed following mouse molar tooth transplantation

1976

J Oral Pathology Medicine

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Abstract. The enamel organs of subcutaneously transplanted mouse molar teeth undergo cyst formation and present a model for the study of the pathogenesis of developmental odontogenic cysts. Maxillary molars extracted from 10‐day‐old C57B1 mice were grafted subcutaneously into adult mice of the same strain. The grafts were recovered between 2 and 50 days after operation and routinely prepared for histological examination. After initial degeneration, the enamel organ took the form of a squamous epithelium in which after 5 to 6 days squamous hyperplasia took place. Cystic degeneration within the epithelium produced cavities lined with a thick parakeratotic stratified squamous epithelium which resembled the lining of an epidermoid implantation cyst. As the cysts enlarged the lining changed in appearance to a thin squamous non‐keratinized epithelium. Similar features have been described in other experimentally produced cysts and in some odontogenic cysts in humans. Their different morphology may arise from interaction between the surrounding connective tissue with epithelium at different stages of development.

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“…The production of cyst models for experimental research has been undertaken by several researchers [15][16][17][18][19] . These have been used to investigate cancer metastasis 15 and angiogenesis 17 .…”

Section: Discussionmentioning

confidence: 99%

“…These have been used to investigate cancer metastasis 15 and angiogenesis 17 . Thompson and van Wyk 18 produced cysts by implanting vaginal epithelial tissue into the flanks of nude mice, and Soskolne et al 16 implanted gingival tissue into the mandible of rats. In both of these cases, the implants formed cysts.…”

Section: Discussionmentioning

confidence: 99%

Transplantation of oral mucosa explants using different biological and synthetic membranes in an attempt to achieve one‐stage reconstruction of soft‐tissue defects

et al. 2012

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Aim: The purpose of this study was to assess the effectiveness of combining freshly excised oral mucosa tissue with different biological and synthetic scaffolds for production of graft material for one‐stage soft‐tissue reconstruction. Material and Methods: Small oral mucosa biopsies were finely minced and transplanted onto a larger circular subcutaneous wound bed in rats. Test groups included explants seeded onto (1) an electrospun scaffold, (2) acellular dermis, and (3) directly on the muscle fascia. The soft‐tissue implants were examined using histology and immunohistochemical analysis for cytokeratins (CKs) 5, 10, 13, and 19. Results: Mucosal explants grew into fully developed epithelium with keratinisation. Surprisingly, instead of spreading over the surface of the membranes, the epithelial explants curled up and formed cyst‐like structures in all test groups. The epithelial layer of the cysts originated from mucosal explants grown on the electrospun scaffold showed weak positive staining for CK5, CK10, and CK19 similar to native rat oral mucosa. Other groups showed negative staining for the CKs. Conclusions: Subcutaneous transplantation of freshly excised oral mucosa explants resulted in the growth of the explants and formation of inclusion cyst‐like structures. The phenotype of the cyst epithelium was affected by the underlying connective tissue substrate.

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Production of keratinizing cysts within mandibles of rats with autogenous gingival epithelial grafts

Cited by 16 publications

References 13 publications

Different Histogenesis of Experimental Odontogenic Cysts by the Renal Subcapsular Transplantation of Tooth Germs of Mice.

Different Histogenesis of Experimental Odontogenic Cysts by the Renal Subcapsular Transplantation of Tooth Germs of Mice.

A histological study of odontogenic cysts formed following mouse molar tooth transplantation

Transplantation of oral mucosa explants using different biological and synthetic membranes in an attempt to achieve one‐stage reconstruction of soft‐tissue defects

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