Production of keratinolytic enzymes in a rotating frame bioreactor

Shama, Gilbert; Berwick, Paul G.

doi:10.1007/bf00185014

Cited by 13 publications

(8 citation statements)

References 3 publications

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“…Lin et al (1992) reported that the keratinolytic activity by B. licheniformis PWD-1 was increased with cultivation time and observed a maximum activity at the late logarithmic growth phase. The same result was reported on Streptomyces fradiae (Shama and Berwick 1991). Those results were similar to that observed in B. subtilis S8 (Fig.…”

Section: Discussionsupporting

confidence: 93%

Characterization of a multifunctional feather-degrading Bacillus subtilis isolated from forest soil

et al. 2010

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In this study, we isolated and characterized a novel feather-degrading bacterium that shows keratinolytic, antifungal and plant growth-promoting activities. A bacterium S8 was isolated from forest soil and confirmed to belong to Bacillus subtilis by BIOLOG system and 16S rRNA gene analysis. The improved culture conditions for the production of keratinolytic protease were 0.1% (w/v) sorbitol, 0.3% (w/v) KNO(3), 0.1% (w/v) K(2)HPO(4), 0.06% (w/v) KH(2)PO(4) and 0.04% (w/v) MgCl(2)·6H(2)O (pH 8.0 and 30°C), respectively. In the improved medium containing 0.1% (w/v) feather, keratinolytic protease production was around 53.3 ± 0.3 U/ml at 4 day; this value was 10-fold higher than the yield in the basal feather medium (5.3 ± 0.1 U/ml). After cultivation for 5 days in the improved medium, intact feather was completely degraded. Feather degradation resulted in free -SH group, soluble protein and amino acids production. The concentration of free -SH group in the culture medium was 15.5 ± 0.2 μM at 4 days. Nineteen amino acids including all essential amino acids were produced in the culture medium; the concentration of total amino acid produced was 3360.4 μM. Proline (2809.9 μM), histidine (371.3 μM) and phenylalanine (172.0 μM) were the major amino acids released in the culture medium. B. subtilis S8 showed the properties related to plant growth promotion: hydrolytic enzymes, ammonification, indoleacetic acid (IAA), phosphate solubilization, and broad-spectrum antimicrobial activity. Interestingly, the strain S8 grown in the improved medium produced IAA and antifungal activity, indicating simultaneous production of keratinolytic and antifungal activities and IAA by B. subtilis S8. These results suggest that B. subtilis S8 could be not only used to improve the nutritional value of feather wastes but also is useful in situ biodegradation of feather wastes. Furthermore, it could also be a potential biofertilizer or biocontrol agent applicable to crop plant soil.

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Section: Discussionsupporting

confidence: 93%

Characterization of a multifunctional feather-degrading Bacillus subtilis isolated from forest soil

et al. 2010

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“…The strain kr10 produced an extracellular keratinase, whose levels were variable during cultivation on native feathers. These results resemble those observed for cultures of S. fradiae (Shama and Berwick 1991), S. pactum (Böckle et al. 1995) and Vibrio sp.…”

Section: Discussionsupporting

confidence: 87%

Characterization of a protease of a feather-degrading Microbacterium species

Thys

Lucas

Riffel

et al. 2004

Lett Appl Microbiol

114

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Aims: To characterize a new feather‐degrading bacterium. Methods and Results: The strain kr10 producing a high keratinolytic activity when cultured on native feather broth was identified as Microbacterium sp., based on phenotypical characteristics and 16S rDNA sequence. The bacterium presented optimum growth and feather‐degrading activity at pH 7·0 and 30°C. Complete feather degradation was achieved during cultivation. The keratinase was partially purified by gel filtration chromatography. It was optimally active at pH 7·0 and 55°C. The enzyme was inhibited by 1,10‐phenanthroline, EDTA, p‐chloromercuribenzoic acid, 2‐mercaptoethanol and metal ions like Hg2+, Cu2+ and Zn2+. Significance and Impact of the Study: A new Microbacterium sp. strain was characterized presenting high feather‐degrading activity, which appears to be associated to a metalloprotease‐type keratinase. This micro‐organism has enormous potential for use in biotechnological processes involving keratin hydrolysis.

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“…Thiol formation by strain kr2 suggests the presence of disulphide reductase activity. Reduction of disulphide bridges was also observed for S. pactum grown on feather (Böckle and Müller 1997) and S. fradiae on wool (Shama and Berwick 1991). In addition, the use of reducing agents to enhance keratin degradation by keratinases has been described (Böckle et al .…”

Section: Discussionmentioning

confidence: 75%

Feather keratin hydrolysis by a Vibrio sp. strain kr2

Sangali¹,

Brandelli²

2000

J Appl Microbiol

206

176

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The aim of the study was to characterize feather‐degrading bacteria isolated from poultry industry waste. A Vibrio sp. strain kr2 producing a high keratinolytic activity when cultured on native feather‐containing broth was isolated. The bacterium grew with an optimum at pH 6·0 and 30 °C, where maximum feather‐degrading activity was also observed. Keratinase production was similar at both 25 and 30 °C, while the maximum concentration of soluble protein was reached at 30 °C. Reduction of disulphide bridges was also observed, increasing with cultivation time. The keratinase of strain kr2 was active on azokeratin, azocasein, benzoyl‐arginine‐p‐nitroanilide and Ala‐Ala‐p‐nitroanilide as substrates. The amino acid composition of the feather hydrolysate was determined, presenting similarities with that reported for feather lysate, feather meal and raw feathers. A novel feather‐degrading bacterium was isolated and characterized, showing high keratinolytic activity. Complete feather degradation was achieved during cultivation. Strain kr2 shows potential for use for biotechnological processes involving keratin hydrolysis.

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Production of keratinolytic enzymes in a rotating frame bioreactor

Cited by 13 publications

References 3 publications

Characterization of a multifunctional feather-degrading Bacillus subtilis isolated from forest soil

Characterization of a multifunctional feather-degrading Bacillus subtilis isolated from forest soil

Characterization of a protease of a feather-degrading Microbacterium species

Feather keratin hydrolysis by a Vibrio sp. strain kr2

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