Production of L(+) lactic acid from cassava starch hydrolyzate by immobilized Lactobacillus delbrueckii

Abstract

A process based on low cost production media was developed for fermentative production of L(+) lactic acid. Various process parameters were optimized for the production of L(+) lactic acid from cassava starch hydrolyzate-based medium by immobilized whole cells of Lactobacillus delbrueckii. The parameters considered for optimization were concentration of alginate and CaCl(2), initial cell concentration, curing time, bead size and incubation time of fermentation. Reusability of immobilized cells up to six batches was observed without any decline in lactate production. Lactate yield for L. delbrueckii was 0.93 g lactic acid x g(-1) reducing sugar with a production rate of 0.33 g x l(-1) x h(-1). A column bioreactor packed with immobilized L. delbrueckii was run for three weeks in a continuous mode with a lactate yield of 0.75-0.95 g lactic acid x g(-1) reducing sugar with an average production rate of 0.48 g x l(-1) x h(-1). The study revealed that agro residues like cassava bagasse could be used as source material for developing a low cost technology for lactate production.

L(+)-Lactic acid recovery from cassava bagasse based fermented medium using anion exchange resins

“…On industrial point of view, water may be selected as a solvent for washing or washing step can be avoided to reduce the cost. Cassava bagasse, the agro industrial waste was proved as a potential carbon source for the production of lactic acid through submerged, solid-state and simultaneous sachharification fermentation (Rojan et al, 2005;John et al, 2006a;2006b;2006c;2007b;2007c;2007d). Simultaneous saccharification fermentation has several advantages over solid-state and submerged fermentation using the hydrolyzate (John et al, 2006b;2006c;2007d).…”

The use of microorganisms in increasing the protein yield of cassava (Manihot esculenta Crantz) peel wastes

“…Abilities of some lactic acid bacteria to grow in hydrolyzed cassava peel waste (CPW) indicated Lactobacillus delbrueckii NRRL B-763 to produce the highest biomass on cassava peels and was, therefore, selected for mixed culture fermentation of the peel (Table 2). Table 2 shows the growth rates of Lactobacillus delbrueckii NRRL B-763 measured as an increase in protein content of the CPW after 72 h. This organism has been used for protein enrichment of cassava foods 19 and has also been used to produce lactic acid from cellulose waste materials 20, 21 .…”

Strain improvement of Lactobacillus delbrueckii using nitrous acid mutation for l-lactic acid production

“…They can be replaced by various cheap and abundant substrates like agro-industrial wastes or by-products (John et al 2007b). Cassava bagasse, a starchy waste, can be effectively utilized for lactic acid production (Rojan et al 2005;John et al2006;2007a;c;d).…”

“…Lactic acid and its derivatives are widely used in food, pharmaceutical and cosmetic industries (Rojan et al 2005;John et al 2007a;b). Besides the conventional applications, lactic acid has emerged as a raw material for many organic chemicals such as polylactic acid (PLA), a biodegradable polymer used as environmentally friendly plastic (John et al 2007a, b, c;Patnaik et al 2002).…”