Production of laccase by a newly isolated strain of Trametes modesta

Nyanhongo, Gibson S.; Gomes, Joseph; Gübitz, Georg; Zvauya, R.; Read, John S.; Steiner, Walter

doi:10.1016/s0960-8524(02)00044-5

Cited by 123 publications

(76 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The optima pH for laccase was determined by incubating the enzyme with their appropriate substrates at their optimum temperature using Mcllvaine's method [17].…”

Section: Effect Of Ph On Enzymes Productionmentioning

confidence: 99%

Improvement of Laccase Production in Pluerotus pulmonarius-LAU 09 by Mutation

Adebayo¹,

Kola²,

Yadav³

et al. 2012

MICROBIOLOGY

View full text Add to dashboard Cite

The strain improvement of P. pulmonarius LAU 09 for laccase production was carried out by UV-light exposure at 210nm (UV-sterilizer, Millipore xx63 70000) for 90minutes. The highest laccase activity of 2.5 Uml-1 was produced by LAU 90 (mutant) in comparison with obtained yield by wild type LAU 09 (1.75 Uml-1). The optimal physiological parameters for optimum laccase production by LAU 90 are 30OC of temperature, pH of 5.0 and incubation of 168hours. The mutant strain gave the highest total enzyme activity and enzyme specific activity of 71.7 x 10-4 mol/min and 89.63X 10-4 IU/mg of purified laccase enzyme respectively, with molecular weight of 97KDa. The activity of the purified enzyme was strongly inhibited by Sodium azide and EDTA at 1ml/0.1mM and 0.5ml/100mM respectively. The result obtained shown an improved performance of the mutant (LAU 90) strain of P. pulmonarius in laccase production over the wild (LAU 09) type.

show abstract

“…The optima pH for laccase was determined by incubating the enzyme with their appropriate substrates at their optimum temperature using Mcllvaine's method [17].…”

Section: Effect Of Ph On Enzymes Productionmentioning

confidence: 99%

Improvement of Laccase Production in Pluerotus pulmonarius-LAU 09 by Mutation

Adebayo¹,

Kola²,

Yadav³

et al. 2012

MICROBIOLOGY

View full text Add to dashboard Cite

show abstract

“…However, high amounts of the enzyme are needed for these applications, as well as to study and understand its properties and role in lignin biodegradation. In this way, researches have been developed in order to increase laccase production by searching for new sources of it (16,17) and by screening for inducers of laccase production by microorganisms, like aminoacids (8), aromatic compounds (10), plant extracts (2) and copper (9,20). The white rot fungus shiitake (Lentinula edodes (Berk.)…”

Section: Introductionmentioning

confidence: 99%

Screening of inducers for laccase production by Lentinula edodes in liquid medium

Cavallazzi¹,

Kasuya²,

Soares³

2005

Braz. J. Microbiol.

View full text Add to dashboard Cite

Laccases are enzymes involved in lignin degradation and are produced by various organisms. Due to their low substrate specificity their potential to be used in biotechnological applications has received attention. The addition of laccase inducers to the culture medium of microorganisms can enhance laccase production and facilitate its purification and utilization. The aim of this study was to investigate the effect of some compounds as laccase inducers in cultures of Lentinula edodes (shiitake). First, it was selected a culture medium suitable for laccase production by shiitake using two levels of N (2.6 and 26 mM) and seven levels of Cu (0, 50, 100, 150, 200, 250 and 300 µM). The medium with 2.6 mM N and 250 µM Cu was found to provide the highest laccase activity. To the selected medium it were added gallic acid (1 mM), catechol (1 mM), ammonium tartrate (55 µM), hydroxybenzoic acid (1 mM) and vanillin (1 mM). The two first compounds completely inhibited laccase activity and a 30 day time course experiment was carried out with the remaining compounds. Only cultures with ammonium tartrate exhibited laccase activity higher than control cultures, reaching 251 U/ mL of extract after 30 days. A native-PAGE was performed and showed only one band, suggesting that no isozyme was produced.

show abstract

“…Some practical applications include: removal of xenobiotics from aqueous streams (4), removal of phenolic compounds from wine (26), biosensors (34), decolorisation of dyes (27) and effluents contaminated with industrial wastes (23), drug analysis (1), ethanol production (14) and removal of lignin from woody tissues (2). The wide range of substrates that can be attacked by laccases has led to a search for new sources of the enzyme (11,20,21). Lepista sordida (Schum.…”

mentioning

confidence: 99%