Production of laccase from <i>Trametes trogii</i> TEM H2: a newly isolated white‐rot fungus by air sampling

Koçyi̇ği̇t, Ali; Pazarbaşi, M. Barış; Yasa, I Wayan Putu Sutirta; Özdemir, Güven; Karaboz, İsmail

doi:10.1002/jobm.201100341

Cited by 24 publications

(13 citation statements)

References 44 publications

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“…Janusz et al [28] have reported pH 7.5, optimum for production of laccase by Rhizoctonia praticola , as an exceptional case. In a recent study on Trametes trogii , pH 3 was found to be optimum for laccase production [29]. …”

Section: Discussionmentioning

confidence: 99%

Laccase Production from a Temperature and pH Tolerant Fungal Strain ofTrametes hirsuta(MTCC 11397)

Dhakar

Pandey

2013

Enzyme Research

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Laccase production by a temperature and pH tolerant fungal strain (GBPI-CDF-03) isolated from a glacial site in Indian Himalayan Region (IHR) has been investigated. The fungus developed white cottony mass on potato dextrose agar and revealed thread-like mycelium under microscope. ITS region analysis of fungus showed its 100% similarity with Trametes hirsuta. The fungus tolerated temperature from 4 to 48°C ± 2 (25°C opt.) and pH 3–13 (5–7 opt.). Molecular weight of laccase was determined approximately 45 kDa by native PAGE. Amplification of laccase gene fragment (corresponding to the copper-binding conserved domain) contained 200 bp. The optimum pH for laccase production, at optimum growth temperature, was determined between 5.5 and 7.5. In optimization experiments, fructose and ammonium sulfate were found to be the best carbon and nitrogen sources, respectively, for enhancing the laccase production. Production of laccase was favored by high carbon/nitrogen ratio. Addition of CuSO4 (up to 1.0 mM) induced laccase production up to 2-fold, in case of 0.4 mM concentration. Addition of organic solvents also induced the production of laccase; acetone showed the highest (2-fold) induction. The study has implications in bioprospecting of ecologically resilient microbial strains.

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Section: Discussionmentioning

confidence: 99%

Laccase Production from a Temperature and pH Tolerant Fungal Strain ofTrametes hirsuta(MTCC 11397)

Dhakar

Pandey

2013

Enzyme Research

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“…Lomascolo et al (2003) observed that addition of 0.76 mM ethanol increased laccase activity (266,000 U/L) by Pycnoporus cinnabarinus when compared to other inducers. However, Kocyigit et al (2012) reported that addition of 650 mM ethanol or 1 mM guaiacol in the cultivation of Trametes trogii did not increase laccase activity under both conditions (14,650 U/L and 15,980 U/L, respectively). Most laccase inducers are toxic and expensive.…”

Section: Selection Of Potential Inducer Compounds Of Laccase Activitymentioning

confidence: 91%

Optimum conditions for inducing laccase production in Lentinus crinitus

Valle¹,

Vandenberghe²,

Santana³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Laccases are environmentally friendly alternatives in many important applications such as in bioremediation, biopulping, textile, and the food industry. They have wide substrate specificity, can oxidize a broad range of compounds, and show potential for use in various industrial processes. Therefore, developing methods to increase laccase production is important. In the current study, we aimed to identify optimum conditions for inducing laccase production in the basidiomycete Lentinus crinitus cultivated under varying nitrogen concentrations and in the presence of potential inducers of laccase production, including copper and phenolic compounds. Peak enzymatic activity (11,977 U/L) occurred at higher nitrogen concentrations (2.8 g/L nitrogen). Regardless of the nitrogen concentration, addition of copper increased the laccase activity and decreased mycelial growth, with maximum laccase activity (14,320 U/L) observed at the highest nitrogen concentration combined with 150 mM CuSO 4 . In addition, 8545©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (4): 8544-8551 (2014) Lentinus crinitus laccase production ethanol (0.5 or 1.0 mM) and guaiacol (1.5 mM) increased laccase production to 15,000, 14,800, and 14,850 U/L, respectively. Our findings highlighted the optimum conditions for producing L. crinitus derived laccase as potential alternatives to the conventional production and application of the enzyme.

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“…Copper has been reported to be a strong laccase inducer in several species, for example, Neurospora crassa [136], Paecilomyces sp. WSH-L07 [141], Shiraia bambusicola strain GZ11K2 [142], Trametes trogii TEM H 2 [143], Pleurotus florida NCIM 1243 [106], Peniophora sp. [144], and so forth.…”

Section: Cultural and Nutritional Conditions For Laccase Productionmentioning

confidence: 99%

Fungal Laccases and Their Applications in Bioremediation

et al. 2014

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Laccases are blue multicopper oxidases, which catalyze the monoelectronic oxidation of a broad spectrum of substrates, for example, ortho- and para-diphenols, polyphenols, aminophenols, and aromatic or aliphatic amines, coupled with a full, four-electron reduction of O2 to H2O. Hence, they are capable of degrading lignin and are present abundantly in many white-rot fungi. Laccases decolorize and detoxify the industrial effluents and help in wastewater treatment. They act on both phenolic and nonphenolic lignin-related compounds as well as highly recalcitrant environmental pollutants, and they can be effectively used in paper and pulp industries, textile industries, xenobiotic degradation, and bioremediation and act as biosensors. Recently, laccase has been applied to nanobiotechnology, which is an increasing research field, and catalyzes electron transfer reactions without additional cofactors. Several techniques have been developed for the immobilization of biomolecule such as micropatterning, self-assembled monolayer, and layer-by-layer techniques, which immobilize laccase and preserve their enzymatic activity. In this review, we describe the fungal source of laccases and their application in environment protection.

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Production of laccase from Trametes trogii TEM H2: a newly isolated white‐rot fungus by air sampling

Cited by 24 publications

References 44 publications

Laccase Production from a Temperature and pH Tolerant Fungal Strain ofTrametes hirsuta(MTCC 11397)

Laccase Production from a Temperature and pH Tolerant Fungal Strain ofTrametes hirsuta(MTCC 11397)

Optimum conditions for inducing laccase production in Lentinus crinitus

Fungal Laccases and Their Applications in Bioremediation

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