Production of lactic acid and byproducts from waste potato starch by Rhizopus arrhizus: role of nitrogen sources

Zhang, Zhanying; Jin, Bo; Kelly, Joseph F.

doi:10.1007/s11274-006-9218-1

Cited by 67 publications

(34 citation statements)

References 19 publications

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“…These results could be attributed to C/N rates of culture media. This is because the previous studies exhibited that C/N rate of medium significantly affected lactic acid and biomass production in fermentations of R. oryzae strains [20,21]. The experiments also demonstrated that lactic acid and biomass production could be achieved in LKF medium without additional nitrogen source (0 g/L level of nitrogen sources; Fig.…”

Section: Effect Of Nitrogen Sources and Their Various Concentrations mentioning

confidence: 51%

“…The cations need to be removed after the fermentation, which results in an increase in recovery cost in downstream processing. When CaCO 3 is used, calcium sulfate can be produced while reconverting the lactate to lactic acid, which might cause considerable environmental problems and extra costs [21].…”

Section: Effect Of Calcium Carbonate On L-lactic Acid Synthesis and Cmentioning

confidence: 99%

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L‐lactic acid production by Rhizopus oryzae MBG‐10 using starch‐rich waste loquat kernels as substrate

et al. 2012

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The objective of this work was to perform production of L-lactic acid from starch-rich waste loquat kernels by newly isolated Rhizopus oryzae MBG-10 fungus. Loquat kernel flour (LKF) was used as substrate (mainly as carbon source). The most favorable conditions for L-lactic acid production were LKF concentration of 80 g/L, CaCO 3 concentration of 20 g/L, ammonium sulfate concentration of 3 g/L and incubation time of 108 h. Under these conditions, L-lactic acid and biomass concentrations were 45.4 and 8.2 g/L, respectively, and a-amylase activity was 81.6 U/mL. No significant pH changes were observed in the medium thanks to the buffering capacity of LKF. L-lactic acid could be produced in a single-stage from starch-rich LKF without prior saccharification by the fungus with high amylolytic enzyme activity. This is the first report on use of waste loquat kernels as a lactic acid production substrate.

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Section: Effect Of Nitrogen Sources and Their Various Concentrations mentioning

confidence: 51%

Section: Effect Of Calcium Carbonate On L-lactic Acid Synthesis and Cmentioning

confidence: 99%

L‐lactic acid production by Rhizopus oryzae MBG‐10 using starch‐rich waste loquat kernels as substrate

et al. 2012

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“…The rate of glucose accumulation was given by Eq. 4, where constant coefficients, 1.11 and 1.053, were derived from the complete hydrolysis of 1 g of starch given 1.11 g of glucose [14] and the complete hydrolysis of 1 g of maltose given 1.053 g of glucose [13], respectively. The term r 0 G is known as glucose production rate from reducing sugar and the term r 00 G is the glucose consumption rate.…”

Section: Ssf Model Descriptionmentioning

confidence: 99%

Lactic acid production directly from starch in a starch-controlled fed-batch operation using Lactobacillus amylophilus

Yen

Kang

2010

Bioprocess Biosyst Eng

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Based on the batch results, we constructed a simplified simultaneous saccharification and fermentation (SSF) model for the simulation of lactic acid production directly from unhydrolyzed potato starch using Lactobacillus amylophilus. The results of batch operation at different initial starch concentrations (20, 40 and 60 g/l) indicated that a higher initial starch concentration would lead to a slightly lower productivity, but would largely decrease the yield. Among that, the batch with 20 g/l of initial starch had the maximum productivity and the maximum yield, which would be 0.31 g/(l h) and 98% (g/g), respectively. In view of increasing the productivity and the final lactic acid concentration, a starch-controlled fed-batch operation with 20 g/l of initial starch was performed. It showed the fed-batch operation with starch controlled at 8 ± 1 g/l by adjusting the starch-feeding rate led to the maximum productivity of 0.75 g/(l h) and the yield of 69%.

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“…In industrial fermentations, it is very rare that a conventional chemical defined medium is used. The medium cost is one of the major operational costs, representing 30-40% of the total [49], so the use of cheap and widely available raw materials is an economical requisite and has a substantial impact on overall cost reduction efforts. Considerable interest has been shown in using agricultural product wastes [9] and by-products from food industry as nitrogen or carbon sources [1], as an option to reduce costs.…”

Section: Introductionmentioning

confidence: 99%

Carob pulp as raw material for production of the biocontrol agent P. agglomerans PBC-1

Manso

Nunes

Raposo

et al. 2010

J Ind Microbiol Biotechnol

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Large-scale production has been the major obstacle to the success of many biopesticides. The spreading of microbial biocontrol agents against postharvest disease, as a safe and environmentally friendly alternative to synthetic fungicides, is quite dependent on their industrial mass production from low-cost raw materials. Considerable interest has been shown in using agricultural waste products and by-products from food industry as nitrogen and carbon sources. In this work, carob pulp aqueous extracts were used as carbon source in the production of the biocontrol agent Pantoea agglomerans PBC-1. Optimal sugar extraction was achieved at a solid/liquid ratio of 1:10 (w/v), at 25°C, for 1 h. Batch experiments were performed in shake flasks, at different concentrations and in stirred reactors at two initial inoculums concentrations, 10(6) and 10(7) cfu ml(-1). The initial sugar concentration of 5 g l(-1) allowed rapid growth (0.16 h(-1)) and high biomass productivity (0.28 g l(-1) h(-1)) and was chosen as the value for use in stirred reactor experiments. After 22 and 32 h of fermentation the viable population reached was 3.2 × 10(9) and 6.2 × 10(9) cfu ml(-1) in the fermenter inoculated at 10(6) cfu ml(-1) and 2.7 × 10(9) and 6.7 × 10(9) cfu ml(-1) in the bioreactor inoculated at 10(7) cfu ml(-1). A 78% reduction of the pathogen incidence was achieved with PBC-1 at 1 × 10(8) cfu ml(-1), grown in medium with carob extracts, on artificially wounded apples stored after 7 days at 25°C against P. expansum.

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Production of lactic acid and byproducts from waste potato starch by Rhizopus arrhizus: role of nitrogen sources

Cited by 67 publications

References 19 publications

L‐lactic acid production by Rhizopus oryzae MBG‐10 using starch‐rich waste loquat kernels as substrate

L‐lactic acid production by Rhizopus oryzae MBG‐10 using starch‐rich waste loquat kernels as substrate

Lactic acid production directly from starch in a starch-controlled fed-batch operation using Lactobacillus amylophilus

Carob pulp as raw material for production of the biocontrol agent P. agglomerans PBC-1

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