Production of Ligninolytic Enzymes by White-Rot FungusDatroniasp. KAPI0039 and Their Application for Reactive Dye Removal

Abstract

This study focused on decolorization of 2 reactive dyes; Reactive Blue 19 (RBBR) and Reactive Black 5 (RB5), by selected white-rot fungus Datronia sp. KAPI0039. The effects of reactive dye concentration, fungal inoculum size as well as pH were studied. Samples were periodically collected for the measurement of color unit, Laccase (Lac), Manganese Peroxidase (MnP), and Lignin Peroxidase (LiP) activity. Eighty-six percent of 1,000 mg L −1 RBBR decolorization was achieved by 2% (w/v) Datronia sp. KAPI0039 at pH 5. The highest Lac activity (759.81 UL −1 ) was detected in the optimal condition. For RB5, Datronia sp. KAPI0039 efficiently performed (88.01% decolorization) at 2% (w/v) fungal inoculum size for the reduction of 600 mg L −1 RB5 under pH 5. The highest Lac activity (178.57 UL −1 ) was detected, whereas the activity of MnP and LiP was absent during this hour. The result, therefore, indicated that Datronia sp. KAPI0039 was obviously able to breakdown both reactive dyes, and Lac was considered as a major lignin-degradation enzyme in this reaction.

Potential of white-rot fungi for bioremediation

“…The metabolic potential of fungi has been explored for bioremediation of xenobiotic polymers, such as petroleum hydrocarbons, organochlorine pesticides, and chemical dyes (Vaithanomsat et al, 2010). White-rot fungi can also serve as promising candidates for treatment of waste effluents derived from textile, coal, alcohol distilleries, pulp, and paper industries (Arora and Sharma, 2010).…”

“…KAPI0039 was implicated in decolorization of two reactive dyes, Reactive Blue 19 (RBBR) and Reactive Black 5 (RB5) (Vaithanomsat et al, 2010). The capacity of degradation of aflatoxin B1 and organochlorine pesticides by laccases from white-rot fungi Peniophora sp.…”

“…In this context, the fungi have emerged as a natural tool in bioremediation, due to their enormous capacity of detoxification of pollutants in environment, and the potential to convert toxic substances to less hazardous or non-hazardous forms (Ahuja et al, 2004;Tisma et al, 2010).…”

Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation

“…Optimization of moisture content could be employed for the management and the modification of microorganism metabolic processes [28]. In any SSF method, a vital factor is the incubation time because it has a largeeffect on growth and biosynthesis of the fungus, as well as the production of various primary and secondary metabolites, such as enzymes [29,30]. For optimum production of various enzymes by fungus, the temperatures optima of 25 to 37˝C range have been reported [31,32].…”

Biomechanical Pulping of Poplar with Crude Enzyme Secreted from Trametes sp. lg-9

“…However, pretreatment with whiterot fungi has some disadvantages such as long treatment time, large reaction space, and low efficiency (Zhong et al 2011;Wang et al 2014). Extracellular enzymes secreted by white-rot fungi can be extracted easily and have been applied in pulping and papermaking (Vaithanomsat et al 2010;Järvinen et al 2012). It is important to find new sources of enzymes for bio-mechanical pulping.…”