Abstract:Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus sp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification … Show more
“…It is characteristic of the aromatic groups as proposed by (Roy et al 2013). However, there are also peaks at 1238 and 1118 cm -1 that are likely due to C-O-C vibrations in esters as de ned by (Habib et al 2020). All these observations con rm the lipopeptide nature of the biosurfactant derived from Bacillus mojavensis BI2.…”
Section: Ftir Analysismentioning
confidence: 57%
“…indicating the presence of a carbonyl group C=O (Habib et al 2020, Pueyo et al 2009, Varadavenkatesan &Murty 2013. Nevertheless, sharp absorbance peaks are observed at 1463 cm-1, 1379 cm-1, 2955 cm -1 and 2854 cm -1 and are indicative of aliphatic chains (-CH 3 and -CH 2 -) in Figure 3 as described by (Jemil et al 2017, Roy et al 2013, Varadavenkatesan &Murty 2013, Zhang et al 2016.…”
Lipopeptides Biosurfactants are natural surface-active compounds produced by a variety of microorganisms. They have great interest in environmental, biomedical and agro-industrial fields. However, the high cost of culture media and the low yield of production limit their large-scale production and application. The development of efficient and cost-effective bioprocess became of a great interest for the improvement of the yield of biosurfactants and the decrease of production cost. In this aim, we applied the response surface method to optimize an economic biosurfactant production by a newly isolated strain B. mojavensis BI2 on date syrup called “Luegmi” as unique carbon and nitrogen source. Using a Box-Bhenken design, we studied the effect of three independent variables on lipopeptide production; Leugmi concentration, Na 2 HPO 4 and incubation time. The results of this study showed that Leugmi concentration at 25%, Na 2 HPO 4 at 0.1% and incubation time of 24 hours were optimal conditions for biosurfactant production, with a maximum surface tension decreasing capacity of 55% corresponding to 27 mN/m and oil dispersing of 30 cm 2 corresponding to a diameter of 6 cm. Preliminary characterization of the biosurfactant produced on Luegmi by UV-Spectra and Thin Layer Chromatography showed its lipopeptide characters. Physic-chemical characterization of the produced lipopepetide on Leugmi showed its great surface activities and stabilities at different pH, temperature and salts concentration. The results of this study suggested that Leugmi, an agricultural byproducts can be used as a low-cost substrate to enhance the yield of lipopeptide biosurfactants with great surface activities for potential environmental application.
“…It is characteristic of the aromatic groups as proposed by (Roy et al 2013). However, there are also peaks at 1238 and 1118 cm -1 that are likely due to C-O-C vibrations in esters as de ned by (Habib et al 2020). All these observations con rm the lipopeptide nature of the biosurfactant derived from Bacillus mojavensis BI2.…”
Section: Ftir Analysismentioning
confidence: 57%
“…indicating the presence of a carbonyl group C=O (Habib et al 2020, Pueyo et al 2009, Varadavenkatesan &Murty 2013. Nevertheless, sharp absorbance peaks are observed at 1463 cm-1, 1379 cm-1, 2955 cm -1 and 2854 cm -1 and are indicative of aliphatic chains (-CH 3 and -CH 2 -) in Figure 3 as described by (Jemil et al 2017, Roy et al 2013, Varadavenkatesan &Murty 2013, Zhang et al 2016.…”
Lipopeptides Biosurfactants are natural surface-active compounds produced by a variety of microorganisms. They have great interest in environmental, biomedical and agro-industrial fields. However, the high cost of culture media and the low yield of production limit their large-scale production and application. The development of efficient and cost-effective bioprocess became of a great interest for the improvement of the yield of biosurfactants and the decrease of production cost. In this aim, we applied the response surface method to optimize an economic biosurfactant production by a newly isolated strain B. mojavensis BI2 on date syrup called “Luegmi” as unique carbon and nitrogen source. Using a Box-Bhenken design, we studied the effect of three independent variables on lipopeptide production; Leugmi concentration, Na 2 HPO 4 and incubation time. The results of this study showed that Leugmi concentration at 25%, Na 2 HPO 4 at 0.1% and incubation time of 24 hours were optimal conditions for biosurfactant production, with a maximum surface tension decreasing capacity of 55% corresponding to 27 mN/m and oil dispersing of 30 cm 2 corresponding to a diameter of 6 cm. Preliminary characterization of the biosurfactant produced on Luegmi by UV-Spectra and Thin Layer Chromatography showed its lipopeptide characters. Physic-chemical characterization of the produced lipopepetide on Leugmi showed its great surface activities and stabilities at different pH, temperature and salts concentration. The results of this study suggested that Leugmi, an agricultural byproducts can be used as a low-cost substrate to enhance the yield of lipopeptide biosurfactants with great surface activities for potential environmental application.
“…Based on the arrangement of genes and the action of the enzymes, combinatorial libraries of structures are generated. These tools can be used in conjunction with metabolite extraction and a combination of analytical chemistry techniques such as thin layer chromatography (TLC), fourier-transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization time-of-flight (MALD-TOF) and mass spectrometry (MS) to inform on structure and confirmation of the final product [142] , [143] , [144] . Fig.…”
Section: Discovery Of New Lps: Where Are We Going?mentioning
confidence: 99%
“…Moreover, a Rhodococcus strain, ADL36, also isolated from Antarctic soil has been shown to display biosurfactant activity; with chemical characterization of the compound and the genome sequencing suggesting the production of a LP with potential applications in the bioremediation of hydrocarbons [144] . In another survey, this time utilising metagenomics data, Chen et al .…”
Section: Discovery Of New Lps: Where Are We Going?mentioning
Lipopeptides (LPs) are secondary metabolites produced by a diversity of bacteria and fungi. Their unique chemical structure comprises both a peptide and a lipid moiety. LPs are of major biotechnological interest owing to their emulsification, antitumor, immunomodulatory, and antimicrobial activities. To date, these versatile compounds have been applied across multiple industries, from pharmaceuticals through to food processing, cosmetics, agriculture, heavy metal, and hydrocarbon bioremediation. The variety of LP structures and the diversity of the environments from which LP-producing microorganisms have been isolated suggest important functions in their natural environment. However, our understanding of the ecological role of LPs is limited. In this review, the mode of action and the role of LPs in motility, antimicrobial activity, heavy metals removal and biofilm formation are addressed. We include discussion on the need to characterise LPs from a diversity of microorganisms, with a focus on taxa inhabiting ‘extreme’ environments. We introduce the use of computational target fishing and molecular dynamics simulations as powerful tools to investigate the process of interaction between LPs and cell membranes. Together, these advances will provide new understanding of the mechanism of action of novel LPs, providing greater insights into the roles of LPs in the natural environment.
“…Both strains used in this study were previously isolated from pristine fellfield soils collected from Victoria Island, Antarctica. The strains' remarkable features include the utilisation of diesel [31] and the production of biosurfactant [34,35]. Strain ADL36 also able to utilise waste palm oil as the sole carbon source [36].…”
Section: Growth Of Bacteria On Pp-supplemented Medium and Estimation mentioning
Microplastic pollution is globally recognised as a serious environmental threat due to its ubiquitous presence related primarily to improper dumping of plastic wastes. While most studies have focused on microplastic contamination in the marine ecosystem, microplastic pollution in the soil environment is generally little understood and often overlooked. The presence of microplastics affects the soil ecosystem by disrupting the soil fertility and quality, degrading the food web, and subsequently influencing both food security and human health. This study evaluates the growth and biodegradation potential of the Antarctic soil bacteria Pseudomonas sp. ADL15 and Rhodococcus sp. ADL36 on the polypropylene (PP) microplastics in Bushnell Haas (BH) medium for 40 days. The degradation was monitored based on the weight loss of PP microplastics, removal rate constant per day (K), and their half-life. The validity of the PP microplastics’ biodegradation was assessed through structural changes via Fourier transform infrared spectroscopy analyses. The weight loss percentage of the PP microplastics by ADL15 and ADL36 after 40 days was 17.3% and 7.3%, respectively. The optimal growth in the BH media infused with PP microplastics was on the 40th and 30th day for ADL15 and ADL36, respectively. The infrared spectroscopic analysis revealed significant changes in the PP microplastics’ functional groups following the incubation with Antarctic strains.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
