Production of macrosphelide A by the mycoparasite<i>Coniothyrium minitans</i>

McQuilken, M. P.; Gemmell, J.; Hill, Robert A.; Whipps, J. M.

doi:10.1016/s0378-1097(02)01180-1

Cited by 52 publications

(51 citation statements)

References 26 publications

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“…This confirms the reports of McQuilken et al (2002McQuilken et al ( , 2003 that C. minitans produced antifungal metabolites in media including MCD. One of the antifungal metabolites produced by the European strain IMI134523 of C. minitans was identified as macrosphelide A (McQuilken et al 2003).…”

Section: Discussionsupporting

confidence: 91%

“…The strain Rap-1 was isolated from a sclerotium of S. sclerotiorum collected from a diseased plant of oilseed rape (Brassica napus) near Wuhan, Hubei. Cultural media used in this study were PDA, potato dextrose broth (PDB) and modified Czapek-Dox (MCD) (McQuilken et al 2003). Both PDA and PDB were made of fresh potato using the procedures described by Fang (1998).…”

Section: Fungal Strains and Mediamentioning

confidence: 99%

“…However, McQuilken et al (2002 reported that cell-free filtrates collected from cultures of a European strain of C. minitans could inhibit mycelial growth of S. sclerotiorum. One of the antifungal substances (AFS) produced by this strain was purified and identified as macrosphelide A (McQuilken et al 2003).…”

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confidence: 99%

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Suppression of Sclerotinia sclerotiorum by antifungal substances produced by the mycoparasite Coniothyrium minitans

Yang

Han

et al. 2007

Eur J Plant Pathol

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A study was conducted to investigate production of antifungal substances (AFS) by Coniothyrium minitans (Cm), a mycoparasite of Sclerotinia sclerotiorum (Ss), in modified Czapek-Dox (MCD) broth and potato dextrose broth (PDB), and effects of AFS of Cm on mycelial growth and germination of sclerotia and ascospores of Ss and incidence of leaf blight of oilseed rape caused by Ss. Results showed that mycelial growth of Ss was reduced by 41.6 and 84.5% on 3 day-old cultures grown on potato dextrose agar (PDA) amended with 10% (v v À1 ) of cultural filtrates of Cm grown in MCD (MCD cm ) after incubation for 6 and 15 days, respectively, and by 2.7 and 15.7% on PDA amended with 10% (v v À1 ) of cultural filtrates of Cm grown in PDB for 6 and 15 days, respectively. In addition to retardation of mycelial growth, morphological abnormality of Ss such as hyphal swellings and cytoplasm granulation were also observed in colonies grown on PDA amended with cultural filtrates of MCD cm . Sclerotia of Ss soaked in the filtrates of MCD cm for 24 h remained viable, but their ability to undergo myceliogenic germination on PDA was delayed, compared to sclerotia treated with MCD. Germination of ascospores of Ss was unaffected on PDA amended with 10% of the filtrates of MCD cm . However, germ tubes of Ss were shortened and deformed by the formation of hyphal swellings in the treatment of MCD cm . Treatment of leaves of oilseed rape with cultural filtrates of MCD cm reduced incidence of leaf blight caused by Ss, compared to the controls (water or MCD). This study suggests that AFS produced by Cm plays an important role in the suppression of mycelial growth and germ-tube development of ascospores of Ss and that there is potential for using AFS of Cm to control leaf blight of oilseed rape caused by ascospores of Ss.

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Section: Discussionsupporting

confidence: 91%

Section: Fungal Strains and Mediamentioning

confidence: 99%

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Suppression of Sclerotinia sclerotiorum by antifungal substances produced by the mycoparasite Coniothyrium minitans

Yang

Han

et al. 2007

Eur J Plant Pathol

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“…The results also showed that conidiation of an L-arginine auxotroph could be restored by the NO donor SNP, but not by cGMP. Thus, NO-mediated conidiation has more than one signal pathway, including the cGMP signal pathway and another yet-unknown pathway, and both are essential for conidiation in C. minitans.Coniothyrium minitans is a sclerotial parasite of the notorious plant pathogen Sclerotinia sclerotiorum, and its potential for biological control of Sclerotinia diseases has been well demonstrated in several countries (13,17,21,28,33,34,36,37). Efficient production of conidia will further enhance the potential of C. minitans as a biological control agent.…”

mentioning

confidence: 99%

Cyclic GMP as a Second Messenger in the Nitric Oxide-Mediated Conidiation of the Mycoparasite Coniothyrium minitans

Fù

Xiè

et al. 2010

Appl Environ Microbiol

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Understanding signaling pathways that modulate conidiation of mitosporic fungi is of both practical and theoretical importance. The enzymatic origin of nitric oxide (NO) and its roles in conidiation by the sclerotial parasite Coniothyrium minitans were investigated. The activity of a nitric oxide synthase-like (NOS-like) enzyme was detected in C. minitans as evidenced by the conversion of L-arginine to L-citrulline. Guanylate cyclase (GC) activity was also detected indirectly in C. minitans with the GC-specific inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), which significantly reduced production of cyclic GMP (cGMP). The dynamics of NOS activity were closely mirrored by the cGMP levels during pycnidial development, with the highest levels of both occurring at the pycnidial initiation stage of C. minitans. Furthermore, the NO donor, sodium nitroprusside (SNP), stimulated the accumulation of cGMP almost instantly in mycelium during the hyphal growth stage. When the activity of NOS or GC was inhibited with N-nitro-L-arginine or ODQ, conidial production of C. minitans was suppressed or completely eliminated; however, the suppression of conidiation by ODQ could be reversed by exogenous cGMP. The results also showed that conidiation of an L-arginine auxotroph could be restored by the NO donor SNP, but not by cGMP. Thus, NO-mediated conidiation has more than one signal pathway, including the cGMP signal pathway and another yet-unknown pathway, and both are essential for conidiation in C. minitans.Coniothyrium minitans is a sclerotial parasite of the notorious plant pathogen Sclerotinia sclerotiorum, and its potential for biological control of Sclerotinia diseases has been well demonstrated in several countries (13,17,21,28,33,34,36,37). Efficient production of conidia will further enhance the potential of C. minitans as a biological control agent. Understanding signaling pathways that modulate conidiation of C. minitans will not only facilitate manipulation of the biocontrol agent for commercial use but also advance our understanding of fungal biology.Nitric oxide (NO) is a widespread signaling molecule involved in regulation of a wide range of cellular functions in animals and plants (7). NO synthesis and signaling have been well studied in animals and plants. In mammals, NO plays roles in relaxation of smooth muscle, inhibition of platelet aggregation, neural communication and immune regulation, while in plants NO is involved in disease resistance, abiotic stress, cell death, respiration, senescence, root development, seed germination, and other functions (reviewed in references 6 and 32). NO is also involved in the development of several members of the mycetozoa, such as, Dictyostelium discoideum (10) and Physarum polycephalum (27). The wide variety of effects reflects the basic signaling mechanism that is used by mammals, plants, and virtually all organisms (2). Despite of the extensive research on NO synthesis and signaling processes in animals and plants, our knowledge about NO in fungi is very limi...

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“…Alternaria alternata Inglis and Boland 1992 Aspergillus niger Rai and Saxena 1975 Aspergillus ustus Rai and Saxena 1975 Coniothyrium minitans Tribe 1957;Huang and Hoes 1976;Turner and Tribe 1976;Whipps and Budge 1990;Budge and Whipps 1991;Trutmann et al 1980;Tu 1984;Huang et al 2000;Jones and Whipps 2002;McQuilken et al 2003, McQuilken andChalton 2009;Gerlagh et al 2003;Chitrampalam et al 2008;Jones et al 2011;Zeng et al 2012b;Bitsadze et al 2015;Jones et al 2015 Drechslera sp. …”

Section: Species Referencesmentioning

confidence: 99%

Biological control of the soil-borne fungal pathogen Sclerotinia sclerotiorum –– a review

2018

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Diseases caused by Sclerotinia sclerotiorum (Lib) de Bary are difficult to control and cause increasing losses of horticultural crops worldwide. Reasons of this phenomenon are various: (i) the specialization of crop production that causes the accumulation of the pathogen in the soil; (ii) the lack of a safe and efficient method of soil fumigation; (iii) the specific life cycle of S. sclerotiorum with survival structures (sclerotia), resistant to chemical and biological degradation. Sclerotinia diseases depend on many environmental factors which determine sclerotia survival and ascospores dissemination, because plants are mainly infected by air-borne ascospores from carpogenic germination of sclerotia. Due to the lack of effective synthetic agents for eradication of S. sclerortiorum from soil considerable interest has been focused on biological control, especially the selection of microorganisms with mycoparasitic activity towards S. sclerotiorum sclerotia, that can decrease their number in the soil. In this work we review reports on the use of different antagonistic fungi and bacteria in the control of S. sclerotiorum and discuss the suppressive effect of organic amendments against this soil-borne pathogen.

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Production of macrosphelide A by the mycoparasiteConiothyrium minitans

Cited by 52 publications

References 26 publications

Suppression of Sclerotinia sclerotiorum by antifungal substances produced by the mycoparasite Coniothyrium minitans

Suppression of Sclerotinia sclerotiorum by antifungal substances produced by the mycoparasite Coniothyrium minitans

Cyclic GMP as a Second Messenger in the Nitric Oxide-Mediated Conidiation of the Mycoparasite Coniothyrium minitans

Biological control of the soil-borne fungal pathogen Sclerotinia sclerotiorum –– a review

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