2019
DOI: 10.1002/jobm.201900295
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Production of melanin pigments in saprophytic fungi in vitro and during infection

Abstract: Melanins are one of the great natural pigments produced by a wide variety of fungal species that promote fitness and cell survival in diverse hostile environments, including during mammalian infection. In this study, we sought to demonstrate the production of melanin in the conidia and hyphae of saprophytic fungi, including dematiaceous and hyaline fungi. We showed that a melanin‐specific monoclonal antibody (MAb) avidly labeled the cell walls of hyphae and conidia, consistent with the presence of melanin in t… Show more

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Cited by 17 publications
(12 citation statements)
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“…Melanins have been linked to fungal pathogenesis of plants, particularly during fungal invasion and penetration into the plant for successful turgor development [37]. We previously reported melanogenesis of filamentous fungi by demonstrating the presence of melanins in external layers of hyphal cell walls in both dematiaceous and hyaline fungi during cornea infection [16]. In FK, melanin may protect fungal cells from host defense mechanisms during initial stages of infection.…”
Section: Discussionmentioning
confidence: 97%
See 1 more Smart Citation
“…Melanins have been linked to fungal pathogenesis of plants, particularly during fungal invasion and penetration into the plant for successful turgor development [37]. We previously reported melanogenesis of filamentous fungi by demonstrating the presence of melanins in external layers of hyphal cell walls in both dematiaceous and hyaline fungi during cornea infection [16]. In FK, melanin may protect fungal cells from host defense mechanisms during initial stages of infection.…”
Section: Discussionmentioning
confidence: 97%
“…In addition to transitioning to a hyphal form, during corneal invasion, fungal pathogens utilize a number of virulence strategies to facilitate their proliferation and combat the host defenses within the cornea. Melanins, a group of biopolymers with multifunctionality, are an important virulence factors in diverse human environmental and human pathogenic fungal species [14][15][16][17][18][19]. The diverse functions of melanin enable fungi to survive in harsh conditions both in vitro and in vivo [15,17,20].…”
Section: Introductionmentioning
confidence: 99%
“…line clone 4D1 was maintained in serum free medium (Gibco), and purified by HiTrap column protein G affinity chromatography (GE Healthcare) according to methods previously described 9 . Immunoreactivity determination of MAb 4D1 was carried out using indirect ELISA with various fungal antigens and slide culture based indirect immunofluorescence assay (IFA) 8,43 .…”
Section: Purification Of Monoclonal Antibody 4d1 (Mab4d1) and Specifimentioning
confidence: 99%
“…It is already known that melanin is directly related to fungi survival and pathogenesis [35,36]. It exhibits pronounced antioxidant action, as it easily interacts with free radicals and other reactive species due to the presence of unpaired electrons in its molecule.…”
Section: Structural and Antioxidant Functionsmentioning
confidence: 99%