Production of microbial lipids from optimized waste office paper hydrolysate, lipid profiling and prediction of biodiesel properties

Nair, Anu Sadasivan; Al-Bahry, Saif N.; Gathergood, Nicholas; Tripathi, Bhumi Nath; Sivakumar, Nallusamy

doi:10.1016/j.renene.2019.12.008

Cited by 28 publications

(5 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The gravimetric data were in co-relation to confocal microscopy study (P < 0.05). The lipid productivity of R. pacifica INDKK in AS-PSH (0.058 g/L/h) was higher than the previously reported oleaginous yeasts on different lignocellulosic hydrolysates such as 0.041 g/L/h on waste office paper enzymatic hydrolysate [18], 0.02 g/L/h on saccharified sweet sorghum juice [46,47], and 0.029 g/L/h on corn stover enzymatic hydrolysate [48] as shown in Table 4. To the best of our knowledge, no yeast isolate reported has produced equivalent lipid titre to isolate R. pacifica INDKK on AS-PSH with effective glucose utilization rate.…”

Section: Discussionmentioning

confidence: 62%

“…Therefore, to establish a sustainable microbial lipid production, extra endeavours are requisite such that yeast efficiently utilize renewable and low-cost carbon sources [12]. In recent years, inexpensive lignocellulosic carbon sources like rice straw hydrolysate [13], elephant grass hydrolysate [14], sugarcane bagasse hydrolysate [15], groundnut shell hydrolysate [16], wheat straw [17] and waste office paper hydrolysates [18] have been used for microbial lipid production. But, the conversion of these renewable feedstock like lignocellulosic materials into lipids in a cost-effective manner is a key challenge [19].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

Kumar

Deeba

Sauraj

et al. 2020

Biotechnol Biofuels

View full text Add to dashboard Cite

Background To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. This study is focussed on high-throughput screening of oleaginous yeast by microwave-aided Nile red spectrofluorimetry and exploring pongamia shell hydrolysate (PSH) as a feedstock for lipid production using novel oleaginous yeast Rhodotorula pacifica INDKK. Results A new oleaginous yeast R. pacifica INDKK was identified and selected for microbial lipid production. R. pacifica INDKK produced maximum 12.8 ± 0.66 g/L of dry cell weight and 6.78 ± 0.4 g/L of lipid titre after 120 h of growth, showed high tolerance to pre-treatment-derived inhibitors such as 5-hydroxymethyl furfural (5-HMF), (2 g/L), furfural (0.5 g/L) and acetic acid (0.5 g/L), and ability to assimilate C3, C5 and C6 sugars. Interestingly, R. pacifica INDKK showed higher lipid accumulation when grown in alkali-treated saccharified PSH (AS-PSH) (0.058 ± 0.006 g/L/h) as compared to acid-treated detoxified PSH (AD-PSH) (0.037 ± 0.006 g/L/h) and YNB medium (0.055 ± 0.003 g/L/h). The major fatty acid constituents are oleic, palmitic, linoleic and linolenic acids with an estimated cetane number (CN) of about 56.7, indicating the good quality of fuel. Conclusion These results suggested that PSH and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production.

show abstract

Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

Kumar

Deeba

Sauraj

et al. 2020

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…7, the highest proportion of paper comes from the departmental offices and most of the papers were not fully utilized for printing or writing as observed, indicating non-existence of reuse of paper waste in the university campus. Consequently, the observation of partial utilization of the majority of generated paper waste also positions paper reuse as a good waste reduction strategy for effective solid waste management in UNN in addition to recycling [34][35][36][37]. Moreover, the largest proportion of plastic and other unclassified wastes were found in buildings while the largest proportions of metal, organic and glass were found in cafeteria (Fig.…”

Section: Comparison Of Average Solid Waste Quantities From the Activi...mentioning

confidence: 96%

Analysis of solid waste composition and its treatment potentials in University of Nigeria Enugu State, Nigeria.

Nwoke¹,

Ime²,

Solueke³

et al. 2022

Nig. J. Tech.

View full text Add to dashboard Cite

The aim of the study is to determine the quantity, composition and treatment potentials of solid waste generated in University of Nigeria Nsukka campus. To achieve these, solid waste was sampled for 1 week from Nine (9) academic buildings, sixty-two (62) academic departments administrative offices, eight (8) corridors, and three (3) cafeterias giving a total of 82 waste sampling points. In total, 4821.1kg/week of waste were separated at University of Nigeria. The total quantity of solid waste at collection points were buildings (1034.9 kg/week), corridor (910.7 kg/week), cafeteria (502.5kg/week) and departmental offices (2373 kg/week). The mean quantities of waste generated at collection points were 114.99kg/week, 113.84kg/week, 167.57kg/week and 38.27kg/week for building, corridors, cafeteria and departmental offices respectively. This shows that on the average that the highest quantity of waste is generated from cafeteria. The weighted average amount of solid waste generated in University of Nigeria Nsukka Campus per sampling point is 58.79kg/week. The weighted average percentage of the waste fractions for university of Nigeria Nsukka Campus is 37.66%, 20.23%, 6.65%, 24.86%, 3.33% and 7.28% for paper, plastic, metal, organic, glass and others respectively. The waste fractions are generated in a decreasing order as follows: paper (37.66%), organic (24.86%), plastic (20.23%). metal (6.65%), others (7.28%) and glass (3.33%). The estimated waste generation rate for the university campus was 0.019kg/capita/day from the four sources analyzed based on current student population of 36000 and weekly generation of 4821.1kg. 28.35% of the waste is compostable while 64.49 % is recyclable and these sums up to 92.84% indicating that only a small proportion that is 7.16% of generated waste which can neither be composted or recycled will be diverted to landfill. Therefore, recovery of resources and recycling call for segregation of waste at the source, through providing separate waste containers for different waste types.

show abstract

“…According to the recent literature (Table 1 ), the yeasts C. curvatus [ 63 – 65 , 92 , 93 ], L. starkeyi [ 67 – 70 ], Metschnikowia pulcherrima [ 71 ], Y. lipolytica [ 85 ]; and species belonging to the Rhodosporidium and Rhodotorula genera [ 72 – 74 , 76 – 82 , 94 ] are the main microbial strains being used for microbial lipid production with lignocellulosic biomass residues. These yeast strains have been tested for lipid production using different raw materials, including agricultural wastes (e.g., corn stover, wheat/rice straw, sugarcane bagasse), woody residues (e.g., maple wood, fir wood, eucalyptus) and energy crops (e.g., switchgrass) pretreated with a wide range of technologies including alkali/acid pretreatment, organosolv, oxidising pretreatment and ionic liquids, among other methods.…”

Section: Lignocellulosic Biomass and Industrial Wastes As Raw Materialsmentioning

confidence: 99%

Challenges and prospects of yeast-based microbial oil production within a biorefinery concept

Gallego-García,

Susmozas,

Negro

et al. 2023

Microb Cell Fact

View full text Add to dashboard Cite

Biodiesel, unlike to its fossil-based homologue (diesel), is renewable. Its use contributes to greater sustainability in the energy sector, mainly by reducing greenhouse gas emissions. Current biodiesel production relies on plant- and animal-related feedstocks, resulting in high final costs to the prices of those raw materials. In addition, the production of those materials competes for arable land and has provoked a heated debate involving their use food vs. fuel. As an alternative, single-cell oils (SCOs) obtained from oleaginous microorganisms are attractive sources as a biofuel precursor due to their high lipid content, and composition similar to vegetable oils and animal fats. To make SCOs competitive from an economic point of view, the use of readily available low-cost substrates becomes essential. This work reviews the most recent advances in microbial oil production from non-synthetic sugar-rich media, particularly sugars from lignocellulosic wastes, highlighting the main challenges and prospects for deploying this technology fully in the framework of a Biorefinery concept.

show abstract

Production of microbial lipids from optimized waste office paper hydrolysate, lipid profiling and prediction of biodiesel properties

Cited by 28 publications

References 54 publications

Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

Analysis of solid waste composition and its treatment potentials in University of Nigeria Enugu State, Nigeria.

Challenges and prospects of yeast-based microbial oil production within a biorefinery concept

Contact Info

Product

Resources

About