Production of Monoclonal Antibodies against Excretory-Secretory Products of Adult Male Onchocerca gibsoni

Harnett, William; Macdonald, M.; Preece, Graham; Patterson, M. K.; Parkhouse, R. M. E.

doi:10.2307/3284464

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…Since monoclonal antibody technique was invented by Kohler and Milstein in 1975, it has been widely used in the research on parasitic diseases (Harnett et al, 1997;Chen et al, 2010). In the production of MAbs, the choice of immunogen is the key step.…”

Section: Discussionmentioning

confidence: 99%

Preparation and characterization of monoclonal antibodies against ES antigens of Trichinella spiralis

Cui

Jing

et al. 2014

Helminthologia

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253 SummaryHybridomas secreting monoclonal antibodies (MAbs) against excretory-secretory (ES) antigen of Trichinella spiralis muscle larvae were produced. The 12 monoclones (designated 3A11, 7D9, 7F6, 5A7, 6H7, 39F3, 38H2, 37E7, 35B9, 29A11, 39C9 and 39A6) secreted IgM, IgG3 and IgG2b, respectively. Western blot using T. spiralis ES antigens showed that ten of 12 MAbs recognized the bands between 119.8 -19.3kDa (mainly 68.4 -28.7kDa) and two MAbs (3A11, 37E7) did not recognize any protein constituents of ES antigens. Nine of 12 MAbs also recognized the larval antigens of T. nativa, T. pseudospiralis and T. nelsoni at 40 days post-infection (dpi). No cross-reactions were found with the somatic antigens of adult worms of P. westermani, C. sinensis, and T. solium cysticercus. However, three (3A11, 6H7 and 39A6) were cross-reacted with the somatic antigens of adult worms of S. japonicum. Immunofluorescent location showed that the nine MAbs reacted with the cuticle and internal organs of T. spiralis larvae. Additionally, five and eight MAbs generated in this study can recognize the early antigens of pre-encapsulated T. spiralis larvae at 11 dpi and 13 dpi, respectively. The generation and characterization of the MAbs against T. spiralis ES antigens provide foundation for the development of specific early serodiagnostic techniques for trichinellosis.

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Section: Discussionmentioning

confidence: 99%

Preparation and characterization of monoclonal antibodies against ES antigens of Trichinella spiralis

Cui

Jing

et al. 2014

Helminthologia

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“…Respons antibodi dalam serum hewan terhadap cacing parasitik dapat terbentuk dengan teknik imunisasi hewan dengan ekskretori/sekretori yang dilepaskan oleh cacing. Sifat imunogenik ekskretori/sekretori Onchocerca gibsoni jantan dewasa diekspresikan melalui pembentukan antibodi spesifik pada tikus (Harnett et al, 1997). Yokoi et al (2002) berhasil memproduksi antibodi monoklonal terhadap antigen ekskretori/sekretori larva Toxocara canis (T. canis), IgG bereaksi spesifik dengan protein ekskretori/sekretori dan tidak bereaksi silang dengan antigen T. canis dewasa.…”

Section: Pendahuluanunclassified

RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli

et al. 2013

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ABSTRAKPenelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P<0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli. ____________________________________________________________________________________________________________________ Kata kunci: Ascaridia galli, ekskretori/sekretori, antibodi, ELISA ABSTRACT

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“…Antibodi yang disekresikan dapat berfungsi sebagai media yang mengikat antigen melalui binding site yang spesifik, sekaligus merupakan jembatan yang menghubungkan antigen dengan sel-sel imun atau mengaktifkan komplemen (Roitt dan Delves, 2001). Harnett et al (1997) membuktikan bahwa aplikasi 300 µg ekskretori/sekretori Ochocerca gibsoni jantan dewasa yang diimunisasikan 50 µg setiap hari selama 6 hari berturut-turut dapat memicu respons humoral hewan percobaan. Imunisasi pertama dan kedua antigen diemulsikan FCA dan IFA berturut-turut.…”

Section: Hasil Dan Pembahasan Uji Spesifisitas Igy Secara Kuantitatifunclassified

Kajian Titer Antibodi Pada Yolk dari Ayam yang Diimunisasi Dengan Antigen Ekskretori/Sekretori Stadium L3 Ascaridia galli

et al. 2008

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The purpose of the present study was to trigger humoral immunity of chickens egg yolk exposed to excretory/secretory released in vitro by L 3 stage of Ascaridia galli. Amount of 6 head chickens were devided into two groups. First group, the chickens were not immnunized. Second group, the chickens were immunized with excretory/ secretory. Active immunizations with excretory/ secretory antigen were applied intra muscularly of chickens with an initial dose of 80 µg. The immunizations were repeated three times with dose of each 60 µg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by enzyme linked immunosorbant assay (ELISA). The result showed that antibody in yolk was begun detect with ELISA increased at two weeks after immunization, This study has shown that the excretory/secretory released in vitro by L 3 stage A. galli is capable of stimulating humoral immunity by mean of producing IgY in yolk.

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Production of Monoclonal Antibodies against Excretory-Secretory Products of Adult Male Onchocerca gibsoni

Cited by 5 publications

References 13 publications

Preparation and characterization of monoclonal antibodies against ES antigens of Trichinella spiralis

Preparation and characterization of monoclonal antibodies against ES antigens of Trichinella spiralis

RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli

Kajian Titer Antibodi Pada Yolk dari Ayam yang Diimunisasi Dengan Antigen Ekskretori/Sekretori Stadium L3 Ascaridia galli

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