Production of N-Acetylchitooligosaccharides by Aeromonas Sp. Dyu-Too 7

Abstract

Aeromonas sp. DYU-Too 7 was screened to produce chitinases. The enzymes had an optimal reaction temperature of 50C and an optimal pH of 7.0. The best concentration of chitin in the fermentation process is 4%. Higher concentrations of chitin inhibited microbial growth and influenced the production of reducing sugars, the enzymatic activity and the protein content in the culture. N -acetylglucosamine, the main product in the hydrolysate, was 0.7 g/L at 36 h of cultivation. The concentration of N -acetylchitobiose was 0.16 g/L for the regular chitin broth and 0.28 g/L for the phosphorus-limiting chitin broth, with the highest concentration of N -acetylchitohexaose at about 0.062 g/L in both media. Experimental results showed that cultivation under a phosphorus-limiting condition substantially reduced the production of biomass, chitinases as well as N -acetylchitooligosaccharides.

Partial purification and antifungal profile of chitinase produced by Streptomyces tendae TK-VL_333

“…V2 (Nawani et al 2002), S. aureofaciens CMUAc 130 (Taechowisan et al 2003), and Aeromonas sp. DYU-TOO 7 (Lien et al 2005).…”