Production of neural stem cells from human pluripotent stem cells

Wen, Yu; Jin, Sha

doi:10.1016/j.jbiotec.2014.07.453

Cited by 31 publications

(21 citation statements)

References 33 publications

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“…As multipotent stem cells, NSCs can self-renew and differentiate into various cell types, such as neurons and glial cells [16,17]. NSCs can be collected from brain tissue, genetically reprogrammed from somatic cells [18,19], or even differentiated from embryonic stem cells (ESCs) and iPSCs [17,20]. In adults, NSCs are localized in the sub-ventricular zone (SVZ) and hippocampus [21,22].…”

Section: Neural Stem Cellsmentioning

confidence: 99%

Effects of neural stem cell transplantation in Alzheimer’s disease models

et al. 2020

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Currently there are no therapies for treating Alzheimer's disease (AD) that can effectively halt disease progression. Existing drugs such as acetylcholinesterase inhibitors or NMDA receptor antagonists offers only symptomatic benefit. More recently, transplantation of neural stem cells (NSCs) to treat neurodegenerative diseases, including AD, has been investigated as a new therapeutic approach. Transplanted cells have the potential to replace damaged neural circuitry and secrete neurotrophic factors to counter symptomatic deterioration or to alter lesion protein levels. However, since there are animal models that can recapitulate AD in its entirety, it is challenging to precisely characterize the positive effects of transplanting NSCs. In the present review, we discuss the types of mouse modeling system that are available and the effect in each model after human-derived NSC (hNSC) or murine-derived NSC (mNSC) transplantation. Taken together, results from studies involving NSC transplantation in AD models indicate that this strategy could serve as a new therapeutic approach.

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Section: Neural Stem Cellsmentioning

confidence: 99%

Effects of neural stem cell transplantation in Alzheimer’s disease models

et al. 2020

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“…As shown in Figure a, siRNA was fully complexed at N/P ratios above 8. As excess materials may potentially impose unexpected side effects in vivo, the nanomedicine formed at N/P = 8 was chosen for further experiments. According to the transmission electron microscopy (TEM) observation, the SPION‐free nanocarrier had a typical vesicle structure (Figure b).…”

Section: Resultsmentioning

confidence: 99%

MRI‐Visible siRNA Nanomedicine Directing Neuronal Differentiation of Neural Stem Cells in Stroke

Wang

Zhang

et al. 2018

Adv Funct Materials

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A major challenge in stroke treatment is the restoration of neural circuit in which neuron function plays a central role. Although transplantation of exogenous neural stem cells (NSCs) is admittedly a promising therapeutical means, the treatment outcome is greatly affected due to the poor NSCs differentiation into neurons caused by myelin associated inhibitory factors binding to Nogo-66 receptor (NgR). Herein, a nanoscale polymersome is developed to codeliver superparamagnetic iron oxide nanoparticles and siRNA targeting NgR gene (siNgR) into NSCs. This multifunctional nanomedicine directs neuronal differentiation of NSCs through silencing the NgR gene and meanwhile allows a noninvasive monitoring of NSC migration with magnetic resonance imaging. An improved recovery of neural function is achieved in rat ischemic stroke model. The results demonstrate the great potential of the multifunctional siRNA nanomedicine in stroke treatment based on stem cell transplantation.

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“… 19 Banda and Grabel 20 have been focusing on using monolayer culture to directly differentiate human ESCs into neural progenitors, which includes four typical stages. Wen and Jin 21 have developed a straightforward and useful strategy for the generation of NSCs from both human ESC and iPSC lines. Comparisons of NSC marker expression and morphology have indicated that there are no significant differences between the NSCs derived via EB formation and monolayer culture methods.…”

Section: Strategies For the Isolation And Generation Of Nscsmentioning

confidence: 99%

Current progress in the derivation and therapeutic application of neural stem cells

2017

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Neural stem cells (NSCs) have a unique role in neural regeneration. Cell therapy based on NSC transplantation is a promising tool for the treatment of nervous system diseases. However, there are still many issues and controversies associated with the derivation and therapeutic application of these cells. In this review, we summarize the different sources of NSCs and their derivation methods, including direct isolation from primary tissues, differentiation from pluripotent stem cells and transdifferentiation from somatic cells. We also review the current progress in NSC implantation for the treatment of various neural defects and injuries in animal models and clinical trials. Finally, we discuss potential optimization strategies for NSC derivation and propose urgent challenges to the clinical translation of NSC-based therapies in the near future.

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Production of neural stem cells from human pluripotent stem cells

Cited by 31 publications

References 33 publications

Effects of neural stem cell transplantation in Alzheimer’s disease models

Effects of neural stem cell transplantation in Alzheimer’s disease models

MRI‐Visible siRNA Nanomedicine Directing Neuronal Differentiation of Neural Stem Cells in Stroke

Current progress in the derivation and therapeutic application of neural stem cells

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