Production of nisin and lactic acid from corn stover through simultaneous saccharification and fermentation

Abstract

The aim of this work was to demonstrate the feasibility of nisin and lactic acid production from corn stover through simultaneous saccharification and fermentation using Lactococcus lactis subsp. lactis ATCC 11454. The optimum conditions for nisin and lactic acid production were cellulose concentration of 30 g/L, a cellulase loading of 25 FPU/g cellulose and an initial pH of 6.0 in a 10 g/L yeast extract and 10 g/L peptone broth. The optimized bioprocess achieved nisin titer of 1635 IU/ mL and lactic acid concentration of 15.2 g/L. The results indicated that simultaneous saccharification and fermentation with corn stover is a promising bioprocess for the economic production of nisin and lactic acid simultaneously.

Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation

“…Our results agreed with Yao et al [46]. It is noticeable that this concentration was half the amount previously published by numerous investigators [17,18,49], who expressed that yeast extract at 10 g.L -1 was an appropriate nitrogen source for maximal lactic acid production when almost no sugar was left in the fermentation medium. The presence of nitrogen in the date palm residue where the crude protein concentration of date leaves is about 10.6-16.5% may explain these results [57].…”

“…A significant increase in lactic acid production (56.6 g. L -1 ) was observed with ATDPL at 7.0% cellulose. These results contrast with Cheng et al [49], who reported that only 15.2 g. L -1 lactic acid was produced from corn stover by SSF at 30 g. L -1 cellulose using Lactococcus lactis subsp. lactis ATCC 11454.…”

Production of Bioactive Peptides from Lactic Acid Bacteria: A Sustainable Approach for Healthier Foods

“…In the olive oil extraction process, residues are generated as a revalorization of olive seeds; Esteve, Marina, and García () obtain antioxidant and antihypertensive peptides using different enzymatic treatments. On the other hand, barley extract (Furuta, Maruoka, Nakamura, Omori, & Sonomoto, ) and corn stover (Cheng et al., ) can also support LAB growth and bacteriocin production, with very low protein content. Also, by enzymatic hydrolysis barley protein extract, antioxidant, antidiabetic, and antihypertensive peptides were generated (Alu'datt et al., ), this last bioactivity was also observed in peptides from wheat germ protein also obtained by hydrolytic enzymes but supported by ultrasound influence (Huang, Liu, Ma, & Zhang, ).…”

Capillary electrophoresis method for the analysis of organic acids and amino acids in the presence of strongly alternating concentrations of aqueous lactic acid

“…Da die gebildete Glucose direkt durch die MS-Bakterien zu MS umgewandelt wird werden hohe Konzentrationen von Glucose im Reaktor verhindert (Wang et al, 2019). Aus der technologischen Sichtweise stellt die Aufspaltung der Polysaccharide den zeitlich limitierenden Schritt dar, da die freigesetzte Glucose unmittelbar zu MS verstoffwechselt wird (Cheng et al, 2018 (Deng et al, 2012;Jin et al, 2005). Hinzu kommt eine vereinfachte Trennung der Biomasse von der Fermentationsbrühe aufgrund des Wachstums von Pilzen in weitverzweigten, fadenförmigen Zellen, den sogenannten Myzelien (Park, 2004).…”