Production of novel polyhydroxyalkanoates by<i>Pseudomonas stutzeri</i>1317 from glucose and soybean oil

He, Wennan; Tian, Weidong; Zhang, Guang; Chen, Guoqiang; Zhang, Zengming

doi:10.1111/j.1574-6968.1998.tb13297.x

Cited by 80 publications

(5 citation statements)

References 9 publications

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“…citronellolis, and P. stutzeri have all been observed to accumulate P(3HO) copolymers with varying degrees of other monomeric units present, when grown on a range of carbon sources. In fact, large scale production of mcl-PHA containing 96 mol % of 3HO was reported in 2009 using P.…”

Section: Resultsmentioning

confidence: 99%

Poly-3-hydroxyoctanoate P(3HO), a Medium Chain Length Polyhydroxyalkanoate Homopolymer from Pseudomonas mendocina

et al. 2011

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Pseudomonas mendocina was found to produce a unique homopolymer of poly(3-hydroxyoctanoate), P(3HO), rather than a copolymer, when grown on sodium octanoate as the sole carbon source. Although this polymer has been produced by other organisms, interestingly this is the first time an absolute homopolymer has been produced by a wild type organism. In addition, a detailed study on the effects of different extraction methods on the yield, molecular weight, thermal properties, and lipopolysaccharide content of P(3HO) has been carried out. The organism was able to accumulate P(3HO) up to 31.38% of its dry cell weight within 48 h in mineral salt medium. Characterization of the monomer was carried out using FTIR, GC-MS, (13)C, (1)H, and HSQC NMR spectroscopy. The polymer had a crystallinity of 37.5%, Young's modulus value of 11.6 MPa and contact angle of 77.3°. Microstructural studies of solvent cast polymer films revealed a smooth surface topography with a root-mean-square roughness value of 0.238 μm.

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Section: Resultsmentioning

confidence: 99%

Poly-3-hydroxyoctanoate P(3HO), a Medium Chain Length Polyhydroxyalkanoate Homopolymer from Pseudomonas mendocina

et al. 2011

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“…This is in keeping with results observed in shaken flask experiments . While other species of Pseudomonas have also been shown to accumulate PHA that is predominantly 3HD in composition from non-related substrates, GO16 accumulates a higher level of 3HDD (He et al 1998;Ward et al 2006;Tobin et al 2007). While the predominant monomer of PHA produced from WG as a sole source of carbon was also 3HD, much higher levels of both 3HDD and 3HDDE were observed along with low levels of 3HTD and 3HTDE.…”

Section: Discussionmentioning

confidence: 97%

Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate

Kenny

Nikodinovic-Runic

Kaminsky

et al. 2012

Appl Microbiol Biotechnol

116

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Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.

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“…O in 0.5 N HCl. The strains were incubated for 48 h at 30 • C and 180 rpm, under aerobic conditions [12].…”

Section: Pha Production Assaymentioning

confidence: 99%

Polyhydroxyalkanoates (PHAs) from Endophytic Bacterial Strains as Potential Biocontrol Agents against Postharvest Diseases of Apples

et al. 2023

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Due to the increasing use and accumulation of petrochemical plastics in the environment and the rapid depletion of natural resources, microbial polyhydroxyalkanoates have great potential to replace them. This study provides new insights in the field of obtaining of polyhydroxyalkanoates (PHAs) from endophytic bacterial strains and applying them as potential biocontrol agents against postharvest diseases of apples. Two strains—Pseudomonas flavescens D5 and Bacillus aerophilus A2—accumulated PHAs in amounts ranging from 2.77 to 5.9 g L−1. The potential to use low-cost substrates such as beet molasses and soapstock for PHA accumulation was shown. The PHAs produced by the Ps. flavescens D5 strain had pronounced antagonistic activity against Penicillium expansum (antifungal property = 62.98–73.08%). The use of PHAs as biocontrol agents significantly reduced the severity of apple blue mold, especially in the preventive treatment option.

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Production of novel polyhydroxyalkanoates byPseudomonas stutzeri1317 from glucose and soybean oil

Cited by 80 publications

References 9 publications

Poly-3-hydroxyoctanoate P(3HO), a Medium Chain Length Polyhydroxyalkanoate Homopolymer from Pseudomonas mendocina

Poly-3-hydroxyoctanoate P(3HO), a Medium Chain Length Polyhydroxyalkanoate Homopolymer from Pseudomonas mendocina

Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate

Polyhydroxyalkanoates (PHAs) from Endophytic Bacterial Strains as Potential Biocontrol Agents against Postharvest Diseases of Apples

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