Production of nucleic acid-related substances by fermentative processes. XLIII. Accumulation of 6-azauridine by mutants of Brevibacterium ammoniagenes.

Furuya, Akira; Kato, Fumio; Nakayama, Kiyoshi

doi:10.1271/bbb1961.39.767

Cited by 8 publications

(2 citation statements)

References 2 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accumulation of IMP has been reported in 5 -nucleotidase-lacking mutants of Bacillus subtilis [Fujimoto et al, 1965;Momose et al, 1964], and IMP production by mutants of Corynebacterium (formerly Brevibacterium ) ammoniagenes has been reported [Furuya et al, 1968.]. However, no 5 -nucleotidase or nucleotide efflux carrier has been genetically identified.…”

Section: Discussionmentioning

confidence: 99%

Complete Deficiency of 5′-Nucleotidase Activity in <i>Escherichia coli </i>Leads to Loss of Growth on Purine Nucleotides but Not of Their Excretion

Kakehi

Usuda²,

Tabira³

et al. 2007

Microb Physiol

View full text Add to dashboard Cite

Escherichia coli has many periplasmic phosphatase activities. To test whether it can take up and excrete purine nucleotides, we attempted to completely disrupt periplasmic 5′-nucleotidase activity. A 5′-nucleotidase activity was induced in ushA knockout mutant cells, which lack major 5′-nucleotidase activity, when they were grown with purine nucleotides as the sole carbon source. Using DNA macroarrays to compare global gene expression in wild-type and ushA knockout mutant cells cultured with IMP or GMP as the sole carbon source, we identified two genes that were induced in the ushA knockout mutant cells and encoded signal sequence needed for secretion. One of the genes, aphA, encoded a 5′-nucleotidase activity and was induced by IMP or inosine. An ushA aphA double knockout mutant was shown to be unable to grow on purine nucleotides as the sole carbon source. To investigate the excretion of purine nucleotides, we constructed an ushAaphA double knockout mutant of an inosine-producing strain and found that it accumulated IMP in the medium. In addition, when the guaBA operon was introduced into the ushAaphA double knockout IMP producer, GMP was released into the medium. These observations imply the existence of efflux activity for purine nucleotides in E. coli.

show abstract

Section: Discussionmentioning

confidence: 99%

Complete Deficiency of 5′-Nucleotidase Activity in <i>Escherichia coli </i>Leads to Loss of Growth on Purine Nucleotides but Not of Their Excretion

Kakehi

Usuda²,

Tabira³

et al. 2007

Microb Physiol

View full text Add to dashboard Cite

show abstract

“…Studies on the microbial production of purine nucleosides have been done exclusively in mutants of Bacillus subtilis [5][6][7] and Corynebacterium ammoniagenes. 8,9) But there have been very few reports of overproduction of these substances by Escherichia coli to date, although the biosynthetic pathway for purine nucleotides and the enzymes involved in their biosynthesis have been studied in detail 10) and the genetic information and tools for gene manipulation are abundant. [11][12][13] Hence, in a previous study, we derived inosineproducing mutants from E. coli W3110 (ATCC27325) 14) using genetic manipulation during each step in order to analyze the genes and the functions involved in inosine production.…”

mentioning

confidence: 99%