Production of Nucleic Acid-related Substances by Fermentative Processes

Abstract

The accumulation of 5'-inosinic acid (IMP) by a mutant, KY 13102, induced from Brevibacterium ammoniagenes ATCC 6872 by ultraviolet light irradiation, was examined. Although growth was stimulated by adenine or adenosine, the microorganism showed fair growth in the medium containing amino acids but no adenine. Among six kinds of natural nutrients tested, meat extract and Casamino Acids were suitable for the accumulation of IMP. Manganese ion strongly affected growth, the accumulation of IMP and hypoxanthine, and cell morphology. Among amino acids tested, L-methionine, L-proline, and L-valine stimulated IMP accumulation. In the medium containing 1.0 g of L-proline per liter, 12.8 mg of IMP per ml was accumulated. The mechanism of IMP accumulation by the mutant is discussed.

Production of Flavour‐enhancing Materials by Streptomycetes: Screening for 5′‐Nucleotides

“…However, further tests showed that these nucleotides were not of the 5'-isomers. Interestingly, many strains accumulated substantial amounts of adenylic acid as found by Furuya et al (1963) and Furuya et al (1964). The results reported so far show that the production of flavour enhancing nucleotides by streptomycetes seems not to be of frequent occurrence.…”

“…Previous screening methods (Okabayashi, 1961 ;Arima et al, 1963;Nakayama et al, 1963) have usually employed the isolation of micro-organisms from various sources, their propagation in liquid media and the evaluation of UV absorbing materials prior to the specific determination of the 5'-nucleotide content. Another approach to the efficient screening for the accumulation of nucleotide-like compounds was attempted by a number of workers (Okabayashi & Masuo, 1960;E'umya et al, 1963;Furuya et al, 1964), using a purine dependent auxotroph as indicator strain.…”

Production of Nucleic Acid-Related Substances by Fermentative Processes. XXVIII. Accumulation of 5′ Inosinic Acid by a Manganese-Insensitive Mutant of Brevibacterium ammoniagenes

“…Accumulation of 5' inosinic acid (IMP) by a mutant, KY 13102, of Brevibacterium ammoniagenes was reported in our previous paper (2). During the course of our studies, manganese ion (Mn2+) was found to have striking effects on IMP accumulation at very low concentrations (10" M).…”

“…Assays. Determinations of IMP and hypoxanthine were made by a paper chromatographic method (2) with a solvent system of isobutyric acid-i N ammonia-acetic acid (10:5:1, v/v). Growth was determined by the same method as reported previously (2).…”

Production of nucleic acid-related substances by fermentative processes. XLIII. Accumulation of 6-azauridine by mutants of Brevibacterium ammoniagenes.

“…Meanwhile, it was reported that inosine (IR) was accumulated in place of 5'inosinic acid (5'IMP) by mutants which are devoid of nucleoside degrading activity and induced from 5'IMP accumulating strain of B. ammoniagenes. 7,8) In this report, efficient conversion of 6AU to 6AUR was aimed and a suitable strain was selected from IR produc ing mutants of B. ammoniagenes. Further more, conditions for the conversion and genetical improvement of the strains was carried out, resulting in accumulation of more than 12mg of 6AUR per ml.…”