Production of Nucleic Acid-Related Substances by Fermentation Processes

Furuya, Akira; Abe, S.; Kinoshita, Shukuo

doi:10.1128/aem.20.2.263-270.1970

Cited by 8 publications

(1 citation statement)

References 9 publications

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“…Meanwhile, it was reported that inosine (IR) was accumulated in place of 5'inosinic acid (5'IMP) by mutants which are devoid of nucleoside degrading activity and induced from 5'IMP accumulating strain of B. ammoniagenes. 7,8) In this report, efficient conversion of 6AU to 6AUR was aimed and a suitable strain was selected from IR produc ing mutants of B. ammoniagenes. Further more, conditions for the conversion and genetical improvement of the strains was carried out, resulting in accumulation of more than 12mg of 6AUR per ml.…”

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confidence: 99%

Production of nucleic acid-related substances by fermentative processes. XLIII. Accumulation of 6-azauridine by mutants of Brevibacterium ammoniagenes.

Furuya¹,

Kato²,

Nakayama³

1975

Agricultural and Biological Chemistry

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From the inosine producing mutants of Brevibacterium ammoniagenes, KY 13761 was selected as a strain which produced 6-azauridine from 6-azauracil. The conditions for the conversion were examined and the intermitent feeding of 6azauracil was found effective for the accumulation. In order to increase the accumulation, prototrophic revertants were induced from KY 13761 and KY 13021 was selected. By intermitent feeding of 6-azauracil of a final concentration of 6mg per ml, a maximal accumulation, 12.4mg/ml, of 6-azauridine was obtained with KY 13021.

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confidence: 99%

Production of nucleic acid-related substances by fermentative processes. XLIII. Accumulation of 6-azauridine by mutants of Brevibacterium ammoniagenes.

Furuya¹,

Kato²,

Nakayama³

1975

Agricultural and Biological Chemistry

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Quantitation of intracellular purine intermediates in different Corynebacteria using electrospray LC-MS/MS

et al. 2012

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Intermediates of the purine biosynthesis pathway play key roles in cellular metabolism including nucleic acid synthesis and signal mediation. In addition, they are also of major interest to the biotechnological industry as several intermediates either possess flavor-enhancing characteristics or are applied in medical therapy. In this study, we have developed an analytical method for quantitation of 12 intermediates from the purine biosynthesis pathway including important nucleotides and their corresponding nucleosides and nucleobases. The approach comprised a single-step acidic extraction/quenching procedure, followed by quantitative electrospray LC-MS/MS analysis. The assay was validated in terms of accuracy, precision, reproducibility, and applicability for complex biological matrices. The method was subsequently applied for determination of free intracellular pool sizes of purine biosynthetic pathway intermediates in the two Gram-positive bacteria Corynebacterium glutamicum and Corynebacterium ammoniagenes. Importantly, no ion pair reagents were applied in this approach as usually required for liquid chromatography analysis of large classes of diverse metabolites.

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Enhancing the production of uridine 5′-monophosphate by recombinant Saccharomyces cerevisiae using a whole cell biocatalytic process

Yang

Gao-Xin

Ying

et al. 2011

World J Microbiol Biotechnol

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Attempts were made with success to produce uridine 5 0 -monophosphate (UMP) from orotic acid by a recombinant Saccharomyces cerevisiae strain pYX212-URA5/BJX12, using the whole cell biocatalytic process. URA5 and URA3 genes, encoding orotate phosphoribosytransferase (OPRTase) and orotidine monophosphate decarboxylase (ODCase), respectively were successfully overexpressed in the industrial yeast strain. As a result, S .cerevisae pYX212-URA5/BJX12 exhibited the highest biocatalytic ability, in contrast with the original industrial yeast strain and S. cerevisae pYX212/BJX12 that overexpressed ODCase only. It indicated that the first step of UMP production from orotic acid is a rate-limiting step. Effects of cultivation for the recombinant strain and biocatalytic reaction conditions on UMP production were also investigated. Cultivating the cells in malt extract medium for 36 h in the exponential phase of growth is in favor of converting orotic acid to UMP. To acquire a higher UMP yield, the conditions of the whole cell biocatalytic reaction were optimized and up to 3.8 g l -1 UMP was produced in 24 h consequently. The yield was fivefold higher than the original UMP yield from the industrial yeast. In addition,

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Production of Nucleic Acid-Related Substances by Fermentation Processes

Cited by 8 publications

References 9 publications

Production of nucleic acid-related substances by fermentative processes. XLIII. Accumulation of 6-azauridine by mutants of Brevibacterium ammoniagenes.

Production of nucleic acid-related substances by fermentative processes. XLIII. Accumulation of 6-azauridine by mutants of Brevibacterium ammoniagenes.

Quantitation of intracellular purine intermediates in different Corynebacteria using electrospray LC-MS/MS

Enhancing the production of uridine 5′-monophosphate by recombinant Saccharomyces cerevisiae using a whole cell biocatalytic process

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