2021
DOI: 10.1002/bit.27814
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Production of octanoic acid in Saccharomyces cerevisiae: Investigation of new precursor supply engineering strategies and intrinsic limitations

Abstract: The eight-carbon fatty acid octanoic acid (OA) is an important platform chemical and precursor of many industrially relevant products. Its microbial biosynthesis is regarded as a promising alternative to current unsustainable production methods. In Saccharomyces cerevisiae, the production of OA had been previously achieved by rational engineering of the fatty acid synthase. For the supply of the precursor molecule acetyl-CoA and of the redox cofactor NADPH, the native pyruvate dehydrogenase bypass had been har… Show more

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Cited by 14 publications
(20 citation statements)
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References 55 publications
(117 reference statements)
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“…The promoter pCOX9 was reported to show only 15% of the activity of the native pPGI1 (Keren et al, 2013). Indeed, the promoter replacement decreased the Pgi1 activity, as measured by Wernig et al (2021). According to the hypothesis of increased NADPH-supply, this strategy increased the production of free fatty acids in S. cerevisiae by roughly 25% in their study (Yu et al, 2018).…”
Section: Boosting the Galoa-production On Glucose By Strengthening Th...mentioning
confidence: 92%
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“…The promoter pCOX9 was reported to show only 15% of the activity of the native pPGI1 (Keren et al, 2013). Indeed, the promoter replacement decreased the Pgi1 activity, as measured by Wernig et al (2021). According to the hypothesis of increased NADPH-supply, this strategy increased the production of free fatty acids in S. cerevisiae by roughly 25% in their study (Yu et al, 2018).…”
Section: Boosting the Galoa-production On Glucose By Strengthening Th...mentioning
confidence: 92%
“…If no reaction is provided to reoxidized the NADPH, ∆pgi1 strains often result in a crucial growth defect of the yeast (Boles et al, 1993). In respect of both findings it was shown, that even the downregulation of the PGI1 by replacing the native promoter with a relatively weak promoter was sufficient to further increase the beneficial effect of an overexpressed ZWF1 on the NADPH-accessibility in the cell (Wernig et al, 2021;Yu et al, 2018). A further strategy to improve the NADPH-supply for (heterologous) reactions was demonstrated by overexpression of the NADH-kinase gene POS5 (Gao et al, 2018;Zhao et al, 2015).…”
Section: Implementing Heterologous Reactions Into the Core Metabolism...mentioning
confidence: 96%
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