Pectin is an acidic heteropolysaccharide, a natural high molecular weight compound primarily found in higher plants. It consists of four major structural domains: homogalacturonan (HG), rhamnogalacturonan II (RG-II), rhamnogalacturonan I (RG-I), and xylogalacturonan (XGA). Various methods are currently employed for pectin extraction, including acid extraction, microbial fermentation, microwave-assisted extraction, and ion extraction, each with unique advantages and disadvantages. Pectin is sourced from fruits and vegetables, such as citrus fruits, apples, beets, and carrots. In terms of regulating human health, pectin enhances antioxidant activity, promotes beneficial microorganisms, and stimulates the production of short-chain fatty acids (SCFAs) through microbial metabolism. Additionally, pectin interacts directly with the mucosa, inhibits Toll-like receptor 2 (TLR2) signaling, and modifies the glycosylation of intestinal mucosal proteins. In disease models, pectin shows preventive and therapeutic effects in inflammatory bowel disease, type 2 diabetes, obesity, cardiovascular disease, and cancer. This review covers recent research, summarizing the sources and extraction methods of pectin, and emphasizes its role as a modulator of human health.