Production of pectin lyase by Penicillium griseoroseum as a function of the inoculum and culture conditions

Brumano, Maria Helena Nasser; Coelho, Jorge Luiz Cavalcante; Araújo, Elza Fernandes de; Silva, D. O.

doi:10.1007/bf00327843

Cited by 21 publications

(12 citation statements)

References 9 publications

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“…These species have been extensively studied physiologically, showing clear differences in their answers to the influence of inoculum age and concentration, as the effect of some cultural conditions (growth period, substrate concentration and different carbon sources) on the production of pectin lyase (PL) and polygalacturonase (PG). The P. griseoroseum PL activity was highest with 9-day-old spore, 5x10 4 spores/mL after 48 h of growth and the P. expansum PL activity with 5-day-old spore, 10 6 spores/mL after 72 h of growth (1)(2)(3)10,11,26,27). Several genetic studies have been carried out in our laboratory in order to isolate strains with enhanced pectinolytic enzyme activities.…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Cardoso¹,

Queiroz²,

Pereira³

et al. 2007

Braz. J. Microbiol.

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Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. The only internal transcribed spacer region variation detected between the two species was the additional six initial nucleotides. The analysis by telomeric fingerprinting showed polymorphism between both species and the chromosome minimal numbers estimated were three. The polymorphism observed in the organization of the subtelomeric region in the genome of two Penicillium species within the high homogeneous Penicillium subgenus is for the first time reported and perhaps can be employed in future phylogenetic studies.

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Section: Introductionmentioning

confidence: 99%

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Cardoso¹,

Queiroz²,

Pereira³

et al. 2007

Braz. J. Microbiol.

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“…Para obtenção de ótimos rendimentos, em relação à produção de enzimas, durante o desenvolvimento dos diferentes tipos de cultivos, não só o número de células influencia, mas também o meio usado para fermentação, a temperatura usada para crescimento do microrganismo e a idade do inóculo (Brumano et al, 1993;Papagianni & Moo-Young, 2001). …”

Section: Resultsunclassified

“…principalmente do próprio microrganismo, visto que nos cultivos tipo "sistema fechado" a concentração de nutrientes, biomassa e os metabólitos alteram constantemente, devido ao metabolismo microbiano (Brumano et al, 1993;Teixeira, 1997;Tucker & Thomas, 1994). Nos experimentos realizados com o inóculo mais concentrado (10%) nos meios de cultura testados verificou-se que Candida intermedia apresentou viabilidade diferenciada, ou seja, menor produção de enzima (Figura 2).…”

Section: Seleção De Leveduras Da Região Amazônica Para Produção De Prunclassified

Seleção de leveduras da Região Amazônica para produção de protease extracelular

Neves¹,

Porto

Teixeira³

2006

Acta Amaz.

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RESUMOO objetivo deste trabalho foi identificar 50 leveduras isoladas a partir de diferentes substratos da Região Amazônica e selecionar uma espécie de maior atividade proteolítica. Entre as 26 espécies identificadas predominaram Candida aquatica (12%) e Candida tropicalis (10%). A fermentação submersa foi realizada em Extrato de Malte suplementado com gelatina 1%, Candida intermedia foi a que expressou maior atividade proteolítica, halo = 25 mm e 273 U/mL, crescimento máximo a 30 ºC, viabilidade celular 6,2 x 10 6 UFC, em 48 horas, não demonstrou características de patogenicidade e a melhor densidade do inóculo foi 3%, utilizando-se culturas com 24 horas de crescimento em ágar Malte. PALAVRAS-CHAVESLeveduras, Região Amazônica, Protease Screening of yeasts from Amazon Region for extracellular protease production ABSTRACT The objective of this work was to identify 50 yeasts isolates obtained from differents substracts from Amazon Region and to select one species of higher proteolytic activity. Among the 26 identified species it had predominance of Candida aquatica (12%) and Candida tropicalis (10%). The submerged fermentation was carried out in Malt Extract

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“…Alternative carbon sources, such as sugar cane juice, have been assayed with the object of reducing the production costs of microbial PGs and PLs. The approach of pectinase production, using carbon sources of low cost and other substances to replace the natural inducer, pectin, has been used by several researchers (Brumano et al. 1993; Baracat‐Pereira et al.…”

Section: Discussionmentioning

confidence: 99%

Improved pectinase production in Penicillium griseoroseum recombinant strains

Teixeira

Gonçalves

Queiroz

et al. 2011

Journal of Applied Microbiology

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Aims: To obtain recombinant strains of Penicillium griseoroseum that produce high levels of pectin lyase (PL) and polygalacturonase (PG) simultaneously. Methods and Results: A strain with high production of PL was transformed with the plasmid pAN52pgg2, containing the gene encoding PG of P. griseoroseum, under control of the gpd promoter gene from Aspergillus nidulans. Southern blot analysis demonstrated that all strain had at least one copy of pAN52pgg2 integrated into the genome. The recombinant strain P. griseoroseum T20 produced levels of PL and PG that were 266‐ and 27‐fold greater, respectively, than the wild‐type strain. Furthermore, the extracellular protein profile of recombinant T20 showed two protein bands of c. 36 and 38 kDa, associated with PL and PG, respectively. Conclusions: This recombinant strain T20 produces PL and PG using carbon sources of low costs, and an enzyme preparation that is free of cellulolytic and proteolytic activities. Significance and Impact of the Study: PL and PG play an important role in the degradation of pectin. Owing to their use in the juice and wines industries, there is a growing interest in the inexpensive production of these enzymes. This work describes an efficient system of protein expression and secretion using the fungus P. griseoroseum.

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Production of pectin lyase by Penicillium griseoroseum as a function of the inoculum and culture conditions

Abstract: Optimum activity of an extracellular pectin lyase produced by Penicillium griseoroseum in submerged culture was after 120 h using 0.1% (w/v) citrus pectin as substrate. Sucrose at 0.1% (w/v) stimulated enzyme production and citrus pectin gave the highest activity of enzyme per unit growth.

Cited by 21 publications

References 9 publications

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Seleção de leveduras da Região Amazônica para produção de protease extracelular

Improved pectinase production in Penicillium griseoroseum recombinant strains

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