Production of phenolic compounds in hairy root culture of tartary buckwheat (Fagopyrum tataricum Gaertn)

Kim, Yong Kyoung; Li, Xiaohua; Xu, Hui; Park, Nam Il; Uddin, Romij; Pyon, J.Y.; Park, Sang Un

doi:10.1007/s12892-009-0075-y

Cited by 52 publications

(41 citation statements)

References 29 publications

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“…The present study exhibited that hairy roots contained higher amount of individual phenolic compounds compared to non-transgenic plants. Similarly, phenolic acid derivatives such as chlorogenic acid and quercetin were presented higher amount in hairy roots when compared with wild type roots of F. tataricum (Kim et al, 2009).…”

Section: Comparison Of Individual Phenolic Compounds In Transgenic Hamentioning

confidence: 93%

“…Transformed roots yielded higher levels of catechin and epicatechin than untransformed roots of H. perforatum (Tusevski and Simic, 2013). Kim et al (2009) reported that the rutin and epicatechin contents in hairy roots were several-fold higher than those in non-transgenic roots of Fagopyrum tataricum . Kaempferol, myricetin, naringin, quercetin and rutin have antimicrobial activity against human pathogenic microorganisms with some mechanisms of action such as inhibition of nucleic acid synthesis, cytoplasmic membrane function and energy metabolisms (Cushnie and Lamb, 2005).…”

Section: Comparison Of Individual Phenolic Compounds In Transgenic Hamentioning

confidence: 99%

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Induction of hairy roots by Agrobacterium rhizogenes -mediated transformation of spine gourd ( Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities

Thiruvengadam

Rekha

Chung

2016

Scientia Horticulturae

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a b s t r a c tAn efficient protocol for hairy root induction of spine gourd (Momordica dioica) was established using Agrobacterium rhizogenes (KCTC 2703). This study evaluates the phenolic compound production, antioxidant and antimicrobial (antibacterial, antifungal and antiviral) activities of transgenic hairy root cultures in M. dioica . Hairy roots were induced from leaves, petiole, and internodal explants. Molecular analysis of PCR and gene sequencing using specific primers of rolC and aux1 revealed T-DNA integration in the hairy root clones and RT-PCR analysis confirmed the expression of hairy root inducible genes (rolC and aux1). The greatest biomass accumulation of hairy roots on MS liquid medium supplemented with 3% sucrose was observed at 22 days. Ultra-HPLC was used to compare the individual phenolic compound contents of transgenic and non-transgenic roots. Moreover, transgenic hairy roots efficiently produced several phenolic compounds, such as flavonols, hydroxycinnamic acid and hydroxybenzoic acid derivatives. The total phenolic, flavonoid contents and biological (antioxidant, antibacterial, antifungal and antiviral) activities were higher in hairy roots compared to non-transformed roots. These results demonstrate the greater potentiality of M. dioica hairy root cultures for the production of valuable phenolic compounds and for studies of their biological activity.

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Section: Comparison Of Individual Phenolic Compounds In Transgenic Hamentioning

confidence: 93%

Section: Comparison Of Individual Phenolic Compounds In Transgenic Hamentioning

confidence: 99%

Induction of hairy roots by Agrobacterium rhizogenes -mediated transformation of spine gourd ( Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities

Thiruvengadam

Rekha

Chung

2016

Scientia Horticulturae

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“…Accumulation of phenolic compound in wild and hairy root of F.tataricum was compared [38]. They reported that phenolic content in hairy roots were several fold higher than wild type roots of same species.…”

Section: Resultsmentioning

confidence: 99%

Metabolomic Analysis and Phenylpropanoid Biosynthesis in Hairy Root Culture of Tartary Buckwheat Cultivars

Thwe

Kim

et al. 2013

PLoS ONE

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Buckwheat, Fagopyrum tataricum Gaertn., is an important medicinal plant, which contains several phenolic compounds, including one of the highest content of rutin, a phenolic compound with anti-inflammatory properties. An experiment was conducted to investigate the level of expression of various genes in the phenylpropanoid biosynthetic pathway to analyze in vitro production of anthocyanin and phenolic compounds from hairy root cultures derived from 2 cultivars of tartary buckwheat (Hokkai T8 and T10). A total of 47 metabolites were identified by gas chromatography–time-of-flight mass spectrometry (GC-TOFMS) and subjected to principal component analysis (PCA) in order to fully distinguish between Hokkai T8 and T10 hairy roots. The expression levels of phenylpropanoid biosynthetic pathway genes, through qRT-PCR, showed higher expression for almost all the genes in T10 than T8 hairy root except for FtF3’H-2 and FtFLS-2. Rutin, quercetin, gallic acid, caffeic acid, ferulic acid, 4-hydroxybenzoic acid, and 2 anthocyanin compounds were identified in Hokkai T8 and T10 hairy roots. The concentration of rutin and anthocyanin in Hokkai T10 hairy roots of tartary buckwheat was several-fold higher compared with that obtained from Hokkai T8 hairy root. This study provides useful information on the molecular and physiological dynamic processes that are correlated with phenylpropanoid biosynthetic gene expression and phenolic compound content in F. tataricum species.

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“…Phenolic compound production by hairy root culture of F . tataricum has also been reported (Kim et al. , 2009).…”

Section: Introductionmentioning

confidence: 92%

Herbicidal activity of phenolic compounds from hairy root cultures of Fagopyrum tataricum

Uddin

Won

et al. 2011

Weed Research

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The hairy root of Fagopyrum tataricum (Tartary buckwheat) has been shown to produce allelochemicals that have herbicidal activity. The hairy root culture technique has been previously employed with F. tataricum to isolate several phenolic compounds. The purpose of this study was firstly to optimise the hairy root culture of F. tataricum using different growth media, supplemented with varying concentrations (0.1-1.0 mg L )1 ) of auxin, for phenolic compound production and secondly to evaluate the phenolic compounds for herbicidal activity to different weed species both in growth chamber and in glasshouse studies. The results suggest that hairy roots grown in half-strength Schenk and Hildebrandt medium showed the highest levels of growth (10.8 g L )1 ) and the maximal production of gallic acid, chlorogenic acid and rutin. Among the different auxin treatments, indole-3-butyric acid at 0.5 mg L )1 produced 24% more hairy root mass than the control. Hairy root extracts of F. tataricum suppressed germination and shoot and root growth in tested weeds, with the grass weed species showing greater susceptibility than the broad-leaved weed species in a growth chamber bioassay. In the glasshouse experiments, post-emergence application of the extract on 21-dayold seedlings had a greater inhibitory effect than the pre-emergence application of hairy root extract. Setaria viridis and Aeschynomene indica were the most susceptible species among grass and broad-leaved weed species, respectively. This study demonstrated that hairy root extract of F. tataricum contained allelopathic compounds and that their phytotoxicity varied with weed species.

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Production of phenolic compounds in hairy root culture of tartary buckwheat (Fagopyrum tataricum Gaertn)

Cited by 52 publications

References 29 publications

Induction of hairy roots by Agrobacterium rhizogenes -mediated transformation of spine gourd ( Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities

Induction of hairy roots by Agrobacterium rhizogenes -mediated transformation of spine gourd ( Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities

Metabolomic Analysis and Phenylpropanoid Biosynthesis in Hairy Root Culture of Tartary Buckwheat Cultivars

Herbicidal activity of phenolic compounds from hairy root cultures of Fagopyrum tataricum

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