Production of podophyllotoxin in Linum linearifolium in vitro cultures

Йонкова, Илиана; Antonova, Irina S.; Momekov, Georgi; Fuss, Elisabeth

doi:10.4103/0973-1296.66932

Cited by 28 publications

(26 citation statements)

References 10 publications

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“…Plant material and in vitro cultures:Seeds from wild Linum thracicum ssp. thracicum plants (Linaceae) were collected near Asenovgrad, Bulgaria in July 2012 and used for initiation of aseptic in vitro cultures, as previously described[5]. Cell suspension cultures were cultivated in full strength (Li) and half strength (HS) liquid Murashige and Skoog medium[18] supplemented with 0.4 mg/L α-naphthaleneacetic acid.…”

mentioning

confidence: 99%

Methyl Jasmonate Induces Enhanced Podophyllotoxin Production in Cell Cultures of Thracian Flax (Linum thracicum ssp. thracicum)

Sasheva

Йонкова

Stoilova

2015

Natural Product Communications

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The Linum thracicum ssp. thracicum cell lines developed in this study are a feasible source for the sustainable production of podophyllotoxin, a lignan with an aryltetralin skeleton that is used for the manufacture of the chemotherapeutic drugs etopophos and teniposide. We used mass spectrometry to confirm the presence of the aryltetralin lignan in the thracian flax cell cultures. Next, we explored how changes in the culture medium influenced the podophyllotoxin content. Out of six developed cell lines, four were selected for further experiments and challenged with elicitors. The selected cell lines clustered into two groups: developed in full strength medium (Li) vs developed in half strength medium (HS). While podophyllotoxin production in the Li cell lines was boosted by 80% upon administration of the elicitor methyl jasmonate, the HS lines produced high amounts of the target metabolite triggered by reduced concentration of nutrients and were only slightly influenced by the elicitor.

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confidence: 99%

Methyl Jasmonate Induces Enhanced Podophyllotoxin Production in Cell Cultures of Thracian Flax (Linum thracicum ssp. thracicum)

Sasheva

Йонкова

Stoilova

2015

Natural Product Communications

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“…were found in the concentration of caffeic acid, quercetin, luteolin and MQ between these tissue lines (Table 1). It was previously shown that the production of phenolics in cultures is highly dependent on plant growth regulators 30 and that growth regulators have higher impact on phenolics' production than changes of nutrient salt concentration in basal medium. 24 The results obtained for in vitro grown tissues are in accordance with these findings.…”

Section: Resultsmentioning

confidence: 99%

Phenolic Compounds in Centaurea rupestris Tissues and Their Antiphytoviral Activity

Ćurković‐Perica¹,

Likić²,

Rusak³

2014

Croat. Chem. Acta

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Abstract. Quantitative and qualitative analysis of phenolic compounds in tissue extracts from Centaurea rupestris L. as well as their antiphytoviral activity against Tomato bushy stunt virus was performed. Extracts of flowers, leaves and roots from C. rupestris growing in the wild, as well as extracts from shoots, tissue consisting of callus and shoots and undifferentiated callus grown in vitro, were tested. Between tested extracts predominantly quantitative and only several qualitative differences in phenolics were detected by high performance liquid chromatography. The highest amounts of quercetagetin 3'-methylether-7-O-ß-D-glucopyranoside and quercetin were detected in flowers while in leaves the highest amounts of luteolin, caffeic and p-coumaric acid were detected. Except for roots, antiphytoviral activity of all other extracts was high, inducing virus inhibition, ranging from 43 to 90 %. Simultaneous application of quercetin, caffeic or p-coumaric acid with virus decreased the number of lesions indicating that these substances contribute to the antiphytoviral activity of C. rupestris extracts. (doi: 10.5562/cca2272)

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“…The production of 2,7′‐cyclolignans by plant tissue cultures has been extensively reviewed [12,15,49–52], and different biological systems from quite a large variety of species have been evaluated for their capacity to accumulate 2,7′‐cyclolignans: Podophyllum spp., Linum spp., Haplophyllum patavum, Juniperis chinensis and Callitris drummondii among others.…”

Section: The Tissue Culture Approachmentioning

confidence: 99%

Updated biotechnological approaches developed for 2,7′‐cyclolignan production¹

Lautie

Fliniaux

Villarreal

2010

Biotech and App Biochem

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2,7'-Cyclolignans constitute a group of compounds regularly employed in clinical contexts due to their antiviral and anticancer properties as expressed in a number of already available designed drugs. Possibilities for other therapeutic developments are indicated. All commercial preparations actually originate from a single compound, podophyllotoxin, currently extracted from a few limited-in-abundance species of Podophyllum. This supply problem has stimulated considerable interest in the development of alternative strategies offering greater sustainable availability of the compound at affordable costs. Approaches such as plant breeding and micropropagation of the high-producing Podophyllum species have been explored with the aim of establishing production of these compounds. Since 20 years of exploration of the total chemical synthesis of podophyllotoxin has not proved economically viable, extensive research has now been undertaken to find methods for stimulating the accumulation of 2,7'-cyclolignans using tissue cultures. Both undifferentiated and differentiated cell cultures, mainly from Linum, Podophyllum, Juniperus, Callitris, Anthriscus and Forsynthia genera, have been reported. Although considerable progress has been made concerning the concentration and productivity of certain strains of Linum album, no economically viable production system has been established. Exploration and development of biosynthetical pathways leading to 2,7'-cyclolignans may open paths for future metabolic engineering, such as the bioconversion of deoxypodophyllotoxin to epipodophyllotoxin employing a human hepatic enzyme heterologously expressed in Escherichia coli.

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Production of podophyllotoxin in Linum linearifolium in vitro cultures

Cited by 28 publications

References 10 publications

Methyl Jasmonate Induces Enhanced Podophyllotoxin Production in Cell Cultures of Thracian Flax (Linum thracicum ssp. thracicum)

Methyl Jasmonate Induces Enhanced Podophyllotoxin Production in Cell Cultures of Thracian Flax (Linum thracicum ssp. thracicum)

Phenolic Compounds in Centaurea rupestris Tissues and Their Antiphytoviral Activity

Updated biotechnological approaches developed for 2,7′‐cyclolignan production¹

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Production of podophyllotoxin in Linum linearifolium in vitro cultures

Cited by 28 publications

References 10 publications

Methyl Jasmonate Induces Enhanced Podophyllotoxin Production in Cell Cultures of Thracian Flax (Linum thracicum ssp. thracicum)

Methyl Jasmonate Induces Enhanced Podophyllotoxin Production in Cell Cultures of Thracian Flax (Linum thracicum ssp. thracicum)

Phenolic Compounds in Centaurea rupestris Tissues and Their Antiphytoviral Activity

Updated biotechnological approaches developed for 2,7′‐cyclolignan production1

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Updated biotechnological approaches developed for 2,7′‐cyclolignan production¹