Production of poly(3-hydroxybutyrate) and its copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Erwinia sp. USMI-20

Majid, Mohamed Isa Abdul; Akmal, D; Few, L.L; Agustien, Anthoni; Toh, Minako; Samian, Mohammed Razip; Najimudin, Nazalan; Azizan, M. N. M.

doi:10.1016/s0141-8130(99)00020-3

Cited by 41 publications

(14 citation statements)

References 10 publications

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“…Furthermore, several studies have previously investigated the co-feeding of different substrates in order to both improve growth and PHA accumulation or to tailor PHA composition (Curley et al 1996;Madden et al 1998;Lee et al 1999bLee et al , 2008Majid et al 1999;Sun et al 2007aSun et al , 2009Bengtsson et al 2010). In all of these studies, the yield of PHA has been improved by co-feeding, while the use of PHA related substrates (i.e.…”

Section: Discussionmentioning

confidence: 99%

Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate

Kenny

Nikodinovic-Runic

Kaminsky

et al. 2012

Appl Microbiol Biotechnol

116

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Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.

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Section: Discussionmentioning

confidence: 99%

Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate

Kenny

Nikodinovic-Runic

Kaminsky

et al. 2012

Appl Microbiol Biotechnol

116

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“…USMI-20 from crude palm oil. 55 Wild-type R. eutropha (C. necator) was shown to accumulate the polymer efficiently from waste oils (up to 57% of CDM), 56 waste rapeseed oil (62% of CDM), 57 and waste frying oil. 58 From waste rapeseed oil, and using propanol as 3HV precursor, the same bacterial species produced the copolymer PHBHV at a concentration of 105 g L −1 with a 3HV content of 8 mol%.…”

Section: Waste Lipids As Carbon Sourcementioning

confidence: 99%

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Favaro

Basaglia

Casella

2018

Biofuels Bioprod Bioref

137

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Polyhydroxyalkanoates (PHAs) are a family of biodegradable intracellular polyesters that a number of Eubacteria and Archaea can accumulate for energy and carbon storage. Most of the genetic modifications to the producing bacterial species have been accomplished to clarify basic biochemical, genetic, and metabolic aspects of PHA metabolism. However, due to its plastic‐like properties and complete biodegradability, this bio‐based polymer has attracted the attention of a variety of manufacturers. A number of genetic approaches have therefore been reported, aimed at improving the performance of the microorganisms with a potential for use in a production process. Indeed, genetic tools may find useful applications in all the phases of the PHA production chain, from the isolation and characterization of new microbial strains through all the production steps until they reach the downstream processes. The substrates generally used for PHA production are expensive, so the search for low‐cost feedstock is necessary. These materials, possibly deriving from agri‐food processes, are unfortunately not easily degraded or converted directly into PHAs. Thus, the development of engineered microbes is in progress to process waste streams and covert them to valuable polymers. This review will summarize the most relevant results obtained through genetic engineering tools for the production of PHAs from cheap carbon sources in view of possible industrial applications. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd.

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“…Direct FAME synthesis FAME were synthesized by a one step extraction-transesterification method as described by Majid et al (1999), after suitable modifications. Oils (720 mg) were weighed into clean, 10 mL screw-top glass bottles, to which 4 mL fresh solution of a mixture of methanol, concentrated sulfuric acid, and chloroform (1.7:0.3:2.0 v/v/v) was added.…”

Section: Sample Preparationmentioning

confidence: 99%

Direct FAME synthesis for rapid total lipid analysis from fish oil and cod liver oil

Indarti

Majid

Hashim

et al. 2005

Journal of Food Composition and Analysis

200

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Production of poly(3-hydroxybutyrate) and its copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Erwinia sp. USMI-20

Cited by 41 publications

References 10 publications

Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate

Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Direct FAME synthesis for rapid total lipid analysis from fish oil and cod liver oil

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