Production of polyhydroxyalkanoates and carotenoids through cultivation of different bacterial strains using brown algae hydrolysate as a carbon source

Muhammad, Mehtab; Aloui, Hajer; Khomlaem, Chanin; Hou, Ching T.; Kim, Beom Soo

doi:10.1016/j.bcab.2020.101852

Cited by 23 publications

(16 citation statements)

References 19 publications

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“…The use of genetic manipulation has further increased the commercial potential of C. necator in PHA synthesis. The glucose-utilizing mutant, C. necator NCIMB 11599, was able to accumulate PHA up to 49 wt% of CDW using the brown algae Laminaria japonica biomass as carbon source ( Muhammad et al, 2020 ). Another strain, C. necator PTCC 1615, successfully utilized brown seaweed Sargassum sp.…”

Section: Microalgae and Bacterial Pha Synthesismentioning

confidence: 99%

“…Using L. japonica biomass, Paracoccus sp. LL1 was able to synthesize PHA as well as carotenoids ( Muhammad et al, 2020 ). Fascinatingly, this same species was able to utilize defatted Chlorella biomass to produce 37.4 wt% of CDW of PHA and 6.08 mg·L −1 of carotenoids ( Khomlaem et al, 2021 ).…”

Section: Microalgae and Bacterial Pha Synthesismentioning

confidence: 99%

“…Bacillus pumilus (E10) isolated from the wastewaters of University of Santa Cruz do Sul was able to utilize the hydrolysate of A. platensis biomass in conjunction with glucose and glycerol to produce PHB. The soil bacterium, Bacillus megaterium ALA2, could make use of defatted Chlorella biomass and L. japonica biomass with PHA production of 29.7 wt% and 32 wt% of CDW, respectively ( Muhammad et al, 2020 ; Khomlaem et al, 2021 ).…”

Section: Microalgae and Bacterial Pha Synthesismentioning

confidence: 99%

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Microalgal Biomass as Feedstock for Bacterial Production of PHA: Advances and Future Prospects

Tan

Nadir

Sudesh

2022

Front. Bioeng. Biotechnol.

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The search for biodegradable plastics has become the focus in combating the global plastic pollution crisis. Polyhydroxyalkanoates (PHAs) are renewable substitutes to petroleum-based plastics with the ability to completely mineralize in soil, compost, and marine environments. The preferred choice of PHA synthesis is from bacteria or archaea. However, microbial production of PHAs faces a major drawback due to high production costs attributed to the high price of organic substrates as compared to synthetic plastics. As such, microalgal biomass presents a low-cost solution as feedstock for PHA synthesis. Photoautotrophic microalgae are ubiquitous in our ecosystem and thrive from utilizing easily accessible light, carbon dioxide and inorganic nutrients. Biomass production from microalgae offers advantages that include high yields, effective carbon dioxide capture, efficient treatment of effluents and the usage of infertile land. Nevertheless, the success of large-scale PHA synthesis using microalgal biomass faces constraints that encompass the entire flow of the microalgal biomass production, i.e., from molecular aspects of the microalgae to cultivation conditions to harvesting and drying microalgal biomass along with the conversion of the biomass into PHA. This review discusses approaches such as optimization of growth conditions, improvement of the microalgal biomass manufacturing technologies as well as the genetic engineering of both microalgae and PHA-producing bacteria with the purpose of refining PHA production from microalgal biomass.

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Section: Microalgae and Bacterial Pha Synthesismentioning

confidence: 99%

Section: Microalgae and Bacterial Pha Synthesismentioning

confidence: 99%

Section: Microalgae and Bacterial Pha Synthesismentioning

confidence: 99%

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Microalgal Biomass as Feedstock for Bacterial Production of PHA: Advances and Future Prospects

Tan

Nadir

Sudesh

2022

Front. Bioeng. Biotechnol.

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“…The bacterium uses invertase (EC 3.2.1.26) to hydrolyze sucrose into glucose and fructose and converts these sugars into pyruvate that is subsequently used in the PHB synthesis pathway similar to C. necator ( Kojima et al, 2004 ). It can accumulate PHB to over 70% by weight when cultivated under stress conditions, e.g., nitrogen limitation ( Sawant et al, 2015 ), and has previously been shown to produce comparable amount of PHB as compared with C. necator when grown on the same substrate, e.g., brown algae ( Laminaria japonica ) hydrolysate ( Muhammad et al, 2020 ). PHAs production by Paracoccus sp.…”

Section: Introductionmentioning

confidence: 99%

High Cell Density Cultivation of Paracoccus sp. on Sugarcane Juice for Poly(3-hydroxybutyrate) Production

Moungprayoon

Lunprom

Reungsang

et al. 2022

Front. Bioeng. Biotechnol.

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High cell density cultivation is a promising approach to reduce capital and operating costs of poly (3-hydroxybutyrate) (PHB) production. To achieve high cell concentration, it is necessary that the cultivation conditions are adjusted and controlled to support the best growth of the PHB producer. In the present study, carbon to nitrogen (C/N) ratio of a sugarcane juice (SJ)-based medium, initial sugar concentration, and dissolved oxygen (DO) set point, were optimized for batch cultivation of Paracoccus sp. KKU01. A maximum biomass concentration of 55.5 g/L was attained using the C/N ratio of 10, initial sugar concentration of 100 g/L, and 20% DO set point. Fed-batch cultivation conducted under these optimum conditions, with two feedings of SJ-based medium, gave the final cell concentration of 87.9 g/L, with a PHB content, concentration, and yield of 36.2%, 32.1 g/L, and 0.13 g/g-sugar, respectively. A medium-based economic analysis showed that the economic yield of PHB on nutrients was 0.14. These results reveal the possibility of using SJ for high cell density cultivation of Paracoccus sp. KKU01 for PHB production. However, further optimization of the process is necessary to make it more efficient and cost-effective.

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“…However, recently seaweed has been explored as a potential substrate for PHA production. Studies have shown that bacteria accumulated PHA in a medium containing brown algae (Azizi et al, 2017;Moriya et al, 2020;Muhammad et al, 2020), red algae (Alkotaini et al, 2016;Bera et al, 2015;Sawant et al, 2018), and green seaweed Ulva sp. (Ghosh et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Marine bacteria associated with the green seaweed Ulva sp. for the production of polyhydroxyalkanoates

Gnaim

Polikovsky

Unis

et al. 2021

Bioresource Technology

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The biosynthesis of polyhydroxyalkanoate (PHA) biopolymers from certain marine microbes, associated with green macroalgae Ulva sp., has attracted significant attention. The Ulva sp. is abundant biomass in numerous locations around the world and could be easily cultivated by marine farming. The variety of sugars found in Ulva sp. homogenate could be used as a carbon source for microbial growth and PHA production. In this work, we isolated and explored a series of bacterial strains that function as potential producers of P(3HB), utilizing a range of common sugars found in Ulva sp. Analysis of 16S rDNA gene-sequence revealed that the PHA-producing bacteria were phylogenetically related to species of the genus Cobetia, Bacillus, Pseudoaltermonas, and Sulfitobacter. The highest-yield of P(3HB) was observed in the case of new Cobetia strain, C. amphilecti, with up to 61% (w/w) in the presence of mannitol and 12% (w/w) on Ulva sp. acid hydrolysate as a substrate.

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Production of polyhydroxyalkanoates and carotenoids through cultivation of different bacterial strains using brown algae hydrolysate as a carbon source

Cited by 23 publications

References 19 publications

Microalgal Biomass as Feedstock for Bacterial Production of PHA: Advances and Future Prospects

Microalgal Biomass as Feedstock for Bacterial Production of PHA: Advances and Future Prospects

High Cell Density Cultivation of Paracoccus sp. on Sugarcane Juice for Poly(3-hydroxybutyrate) Production

Marine bacteria associated with the green seaweed Ulva sp. for the production of polyhydroxyalkanoates

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