Production of polyhydroxybutyrate from Acinetobacter nosocomialis RR20 strain using modified mineral salt medium: a statistical approach

Reddy, A. Ranganadha; Peele, K. Abraham; Krupanidhi, S.; Prabhakar, K.; Venkateswarulu, T.C.

doi:10.1007/s13762-018-2102-3

Cited by 11 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bioplastics, unlike plastics made from petroleum, are made up of natural biopolymers that are manufactured and catabolized by a variety of species and do not harm the host organism (Reddy et al, 2019). As a result of stress, these polymers accumulate in microbial cells as storage materials (Bátori et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

A Review on Production of Polyhydroxyalkanoates in Microorganisms

Ranganadhareddy¹

2022

J Biochem Technol

View full text Add to dashboard Cite

Plastic waste is a concern that is growing due to its nondegradable nature. Many species of microorganisms naturally produce polyhydroxyalkanoates, macromolecule-polyesters that may be used to replace conventional plastics. A variety of microorganisms can completely biodegrade PHAs within a year, which is unlike petroleum-derived plastic that takes decades to degrade. Carbon dioxide and water are produced during this biodegradation, which is returned to the environment. Many methods of mass-producing PHAs have been attempted. Genetically modified microorganisms can be used to establish novel production mechanisms. PHA production, as well as the expression of a few genes, must be maximized in the host for this task. Despite significant advances that have been achieved in generating transgenic organisms, acquiring large quantities of PHA at reasonable costs continues to remain a difficulty. The increasing awareness as well as the promising nature of utilizing microorganisms as a source of polyhydroxyalkanoates are highlighted in this review.

show abstract

Section: Introductionmentioning

confidence: 99%

A Review on Production of Polyhydroxyalkanoates in Microorganisms

Ranganadhareddy¹

2022

J Biochem Technol

View full text Add to dashboard Cite

show abstract

“…Sowmya et al, [17] reported maximum PHB yield of 1.2 g/L at optimum conditions of pH 8.0, sodium chloride concentration 20 g l -1 , inoculum size 0.5% (v/v), glycerol 20 g l -1 and 72 h of incubation at 30°C. In comparison with these previous reports, the PHB production from RR20 strain (18)(19)) is said to be higher and raw materials of medium are also expected to reduce the estimated cost of the process.…”

Section: Results and Discussion Face Centred Central Composite Designmentioning

confidence: 79%

Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Reddy¹,

Prabhakar²,

Venkateswarulu³

et al. 2020

CTBP

View full text Add to dashboard Cite

Eco-friendly biopolymers, polyhydroxybutyrate, have been produced by many kinds of bacteria that possess most important applications in food packaging industries and also in medical field. In the present work, PHB production was economized through the statistical optimization of physical variables. The physical variables chosen for the enhanced production of PHB were incubation period, temperature, pH and inoculums size. The relative rate of production has been studied by understanding the complex interactions of variables using face-centred central composite design. The submerged fermentation with Acinetobacter nosocomialis RR20 produced PHB yield of 5.88 g/L at optimized conditions with a notable value change of sixfold increase in comparison with minimal salt medium and these findings have showed that the designed medium was significant in terms of higher of PHB production.

show abstract

“…Crude PHB was extracted in centrifuge tubes as a pellet and was dried using hot air woven. Earlier researchers reported the accumulation of PHB granules by a large number of gram positive and gram negative bacterial species [27][28][29][30][31][32].…”

Section: Extraction Of Phbmentioning

confidence: 99%

Screening and Biochemical Characterization of PHB Producing Bacterium Isolated from Costal Region of Andhra Pradesh

Aluru¹

2020

EESRJ

View full text Add to dashboard Cite

Biopolymers and their composites have been massively investigated in recent years for multiple applications especially in environmental medical and pharmaceutical fields. A thermoplastic that is both biodegradable and environmental friendly, as well as biocompatible is PHB. PHB is an intracellular granule, which is a carbon and energy reservoir for the bacteria under starvation or stress conditions. In the present work, we focused on identification of potential PHB (Polyhydroxybutyrate) producing bacterial strains from sewage soil of coastal region of Andhra Pradesh. Five bacterial isolates were identified through Sudan Black staining, out of which RR02 was observed to be potential for PHB production. Further, PHB extraction was performed by solvent extraction method. The extracted sample was characterized by FTIR, melting temperature Tm, was determined by DSC and then, compared with standard PHB for confirmation of quality. Biochemical characterization was also performed for preliminary identification of the bacterial isolate. Based on the study it was found to be Bacillus sp.

show abstract

Production of polyhydroxybutyrate from Acinetobacter nosocomialis RR20 strain using modified mineral salt medium: a statistical approach

Cited by 11 publications

References 19 publications

A Review on Production of Polyhydroxyalkanoates in Microorganisms

A Review on Production of Polyhydroxyalkanoates in Microorganisms

Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Screening and Biochemical Characterization of PHB Producing Bacterium Isolated from Costal Region of Andhra Pradesh

Contact Info

Product

Resources

About