Production of polyunsaturated fatty acids in recombinant Lipomyces starkeyi through submerged fermentation

Salunke, Devyani; Manglekar, Rupali Rahul; Gadre, R. V.; Nene, Sanjay; Harsulkar, Abhay

doi:10.1007/s00449-015-1382-y

Cited by 21 publications

(11 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the engineered Y. lipolytica strains with overexpressed heterologous DGA1 genes (R. toruloides DGA1, L. starkeyi DGA1) showed their lipid levels were approximately three-fold higher than the wild type and were also higher than transformants overexpressing the native Y. lipolytica DGA1 gene (Salunke et al, 2015;Zhang et al, 2016). In this study, we showed that DGA1 overexpression resulted in the lipid content of 22.56%, exhibiting a two-fold improvement over control (Figure 2A).…”

Section: Discussionmentioning

confidence: 62%

See 1 more Smart Citation

Overexpression of △12, △15-Desaturases for Enhanced Lipids Synthesis in Yarrowia lipolytica

et al. 2020

View full text Add to dashboard Cite

Microbial oil triacylglycerol (TAG) from the renewable feedstock attract much attention. The oleaginous yeast Yarrowia lipolytica has become the most studied for lipid biosynthesis. Fatty acid desaturases catalyze the introduction of a double bond into fatty-acid hydrocarbon chains to produce unsaturated fatty acids. Desaturases are known to enhance lipid accumulation. In this study, we have achieved a significant increase in lipid production and increase the unsaturated fatty acids content in Y. lipolytica. By comparing the expression of the native genes of-9 stearoyl-CoA desaturase (SCD) and 12 desaturase (12D), and an exogenous 15 desaturase (15D) from flax in the strain with deleted peroxisomal biogenesis factor 10 (PEX10) and overexpressed diacylglyceride acyl-transferase (DGA1), we found that the strain with overexpressed 15 desaturase accumulated 30.7% lipid. Simultaneously, we explored the effect of two copies of desaturase genes (12D-SCD, 15D-SCD, 12D-15D) on lipid production, and found co-expression of 12D and 15D accumulated 42.6% lipid. The lipid content was further increased by 56.3% through the deletion of the multifunctional enzyme (MFE1) and the overexpression of acetyl-CoA carboxylase (ACC1). Finally, the lipid productivity of 50 g/L and maximal lipid content of 77.8% DCW are obtained using a 5-L stirred-tank bioreactor during the stationary phase in the engineered YL-10. Our result demonstrated that the 12 and 15 desaturases play an important role in lipid production in Y. lipolytica and provides an effective strategy for biodiesel development.

show abstract

Section: Discussionmentioning

confidence: 62%

“…Co-expression of 6-and 12-desaturase genes allowed for significant production of γ-linolenic acid (GLA) (Chuang et al, 2010). Additionally, expression of 15 desaturase gene (AEP37840) from flax in Lipomyces starkeyi showed the successful conversion of LA into ALA and fatty acid accumulation (Salunke et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of △12, △15-Desaturases for Enhanced Lipids Synthesis in Yarrowia lipolytica

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The organic nitrogen sources and inorganic nitrogen sources were used to improve PUFA production by SSF using DDGS as the substrate. Some researchers have suggested that different strains have different preferences for either inorganic or organic nitrogen for growth and PUFA production, although organic nitrogen sources are usually used for PUFA production ( 40 ). In our studies, PUFA production was higher when organic nitrogen sources were used ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Enhanced polyunsaturated fatty acids production inMortierella alpinaby SSF and the enrichment in chicken breasts

Yang

Zhang²

2016

Food & Nutrition Research

View full text Add to dashboard Cite

BackgroundDistiller’s dried grains with solubles (DDGS) and soybean meal were used as the substrates for the production of polyunsaturated fatty acids (PUFA) in solid-state fermentation (SSF) by Mortierella alpine. These fermented products were fed to laying hens. PUFA enrichment from chicken breasts was studied.MethodsThe maximum productivity of PUFA was achieved under optimized process condition, including 1% w/w yeast extract as additive, an incubation period of 5 days at 12°C, 10% v/w inoculum level, 75% moisture content, and pH 6.0. The hens were then fed with ration containing soybean DDGS, rapeseed oil, soybean oil, and peanut oil. The control group was fed with basal ration.ResultsUnder the optimal condition, M. alpine produced total fatty acids (TFA) of 182.34 mg/g dry substrate. It has better mycelial growth when soybean meal was added to DDGS (SDDGS). PUFA in fermentation product increased with higher soybean meal content. The addition of 70% soybean meal to DDGS substrate yielded 175.16 mg of TFA, including 2.49 mg eicosapentaenoic acid (EPA) and 5.26 mg docosahexaenoic acid (DHA). The ratios of ω-6/ω-3 found in chicken breasts fat were all lower than that found in control by 36.98, 31.51, 18.15, and 12.63% for SDDGS, rapeseed oil, soybean oil, and peanut oil, respectively.ConclusionsThis study identified an optimized SSF process to maximize PUFA productivity by M. alpine as the strain. This PUFA-enriched feed increased the PUFA contents as well as the proportions of ω-6 and ω-3 in chicken breasts and liver.

show abstract

“…The values of α‐linolenic acid obtained in this work were higher than any other reported in the literature, thus optimization of its production might be useful, as it has been done to other yeast strains. For instance, an increase from 1.99 to 12.29% in α‐linolenic acid content in recombinant yeast L. starkeyi was reported in a previous work, as a consequence of combined techniques of genetic transformation of Δ15 desaturase and bioprocess optimization in a fed‐batch cultivation. In another study, α‐linolenic acid of Y. lipolytica was optimized, showing that agitation speed (possibly the amount of oxygen transferred to cultures) and the nitrogen source impacted the amount of oil produced .…”

Section: Resultsmentioning

confidence: 79%

“…For instance, an increase from 1.99 to 12.29% in α-linolenic acid content in recombinant yeast L. starkeyi was reported in a previous work, 40 tion. 17 Since its metabolism is still unknown and its genome is available in gene banks, it shows potential for further studies as a model to understand lipid accumulation as it has been done for Y. lipolytica.…”

Section: Bioreactor Cultivations Of M Guilliermondii Bi281a For Limentioning

confidence: 67%

Oleaginous yeast Meyerozyma guilliermondii shows fermentative metabolism of sugars in the biosynthesis of ethanol and converts raw glycerol and cheese whey permeate into polyunsaturated fatty acids

Fabricio

Valente

Ayub

2019

Biotechnology Progress

View full text Add to dashboard Cite

We studied the biotechnological potential of the recently isolated yeast Meyerozyma guilliermondii BI281A to produce polyunsaturated fatty acids and ethanol, comparing products yields using glucose, raw glycerol from biodiesel synthesis, or whey permeate as substrates. The yeast metabolism was evaluated for different C/N ratios (100:1 and 50:1). Results found that M. guilliermondii BI281A was able to assimilate all tested substrates, and the most efficient conversion obtained was observed using raw glycerol as carbon source (C/N ratio 50:1), concerning biomass formation (5.67 g·L−1) and lipid production (1.04 g·L−1), representing 18% of dry cell weight. Bioreactors experiments under pH and aeration‐controlled conditions were conducted. Obtained fatty acids were composed of ~67% of unsaturated fatty acids, distributed as palmitoleic acid (C16:1, 9.4%), oleic acid (C18:1, 47.2%), linoleic acid (C18:2 n−6, 9.6%), and linolenic acid (C18:3 n−3, 1.3%). Showing fermentative metabolism, which is unusual for oleaginous yeasts, M. guilliermondii produced 13.7 g·L−1 of ethanol (yields of 0.27) when growing on glucose medium. These results suggest the promising use of this uncommonly studied yeast to produce unsaturated fatty acids and ethanol using cheap agro‐industrial residues as substrates in bioprocess.

show abstract

Production of polyunsaturated fatty acids in recombinant Lipomyces starkeyi through submerged fermentation

Cited by 21 publications

References 18 publications

Overexpression of △12, △15-Desaturases for Enhanced Lipids Synthesis in Yarrowia lipolytica

Overexpression of △12, △15-Desaturases for Enhanced Lipids Synthesis in Yarrowia lipolytica

Enhanced polyunsaturated fatty acids production inMortierella alpinaby SSF and the enrichment in chicken breasts

Oleaginous yeast Meyerozyma guilliermondii shows fermentative metabolism of sugars in the biosynthesis of ethanol and converts raw glycerol and cheese whey permeate into polyunsaturated fatty acids

Contact Info

Product

Resources

About