Production of prebiotic xylooligosaccharides from alkaline extracted wheat straw using the K80R-variant of a thermostable alkali-tolerant xylanase

Faryar, Reza; Linares-Pastén, Javier A.; Immerzeel, Peter; Mamo, Gashaw; Andersson, Maria; Stålbrand, Henrik; Mattìasson, Bo; Karlsson, Eva Nordberg

doi:10.1016/j.fbp.2014.11.004

Cited by 75 publications

(52 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…24 Xylanolytic enzymes that act on the xylan backbone and on the glycosyl decorations can be used to deconstruct xylan polysaccharides into mono-and oligosaccharides that can be further converted into bioethanol and platform chemicals. [25][26][27][28] However, this process drastically reduces the molar mass of the hemicelluloses and restricts their use in biopolymeric material applications. A sequential process that combines subcritical water and selective carbohydrate-active enzymes is here proposed to fractionate polymeric hemicelluloses and oligosaccharides from cereal by-products while preserving their native phenolic functionalities.…”

mentioning

confidence: 99%

Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes

et al. 2017

View full text Add to dashboard Cite

Wheat bran is a major by-product of cereal production that still has limited use for advanced nutritional and material applications. A sequential process using subcritical water, membrane filtration and selective enzymatic treatments has been designed for the combined fractionation of functional high molar mass hemicelluloses (over 10 5 g mol −1 ) and oligosaccharides from wheat bran. This process not only offers increased total solid yield compared with conventional protocols based on alkaline extraction, but it also preserves the inherent functionalities of the phenolic groups that substitute the carbohydrate structures of the extracted hemicelluloses. Feruloylated arabinoxylans (F-AX) with high molar mass and significant radical scavenging activity can be isolated from the subcritical water extract. Structurally different oligosaccharides, including mixed-linkage β-D-glucan oligosaccharides (BGOs) and arabinoxylo-oligosaccharides (AXOs) can be recovered from the eluent after membrane filtration. The crosslinked residue after subcritical water extraction was further treated with xylanolytic enzymes to release valuable feruloylated arabinoxylo-oligosaccharides (FAXOs). The oligo-and polysaccharide fractions isolated from this sequential process show great potential for use as prebiotic or platform chemicals, and as polymeric matrices for carbohydrate-based materials with radical scavenging properties, respectively. IntroductionCereal processing generates large volumes of by-products that have not been exploited to their full potential for advanced nutritional and material applications. 1 Each ton processed by the wheat milling industry generates up to 0.25 tons of wheat bran that corresponds to 14-16% of the cereal kernel. 2 Due to its content of valuable biomacromolecules, such as polysaccharides (mainly hemicelluloses) and phenolic compounds, wheat bran has gained renewed interest as a raw material to be used in second generation biorefineries. 2-4Xylan is one of the most abundant biopolymers in nature as a major non-cellulosic component of plant cell walls. In particular, arabinoxylan (AX) constitutes the main hemicellulose in grasses and cereal grains, with an average composition of 20-40% depending on the species and tissue considered. View Article OnlineView Journal | View Issue substitutions. 9 Other methods have also been developed for the extraction and purification of AX from cereal by-products, including acid pretreatment, 11 microwave assisted extraction, 12 ultrasound assisted extraction, 13,14 and steam explosion extraction. 10,15 As an alternative, subcritical water extraction (SWE), also called pressurized hot water extraction (PHWE), appears as a promising green method for the isolation of hemicellulose fractions with preserved molecular functionalities and high molecular weight. The use of subcritical conditions (at temperature below critical point and pressure high enough to maintain liquid state) changes dramatically the properties of water, including density surface tension, polarity, vis...

show abstract

mentioning

confidence: 99%

Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…viridiscens NCIM2167) and this growth was higher than glucose or control but lower than FOS (Samanta et al, ). Contrary to these results, Faryar et al () showed that L . brevis DSM 1269 could use XOS from wheat straw xylan; however, the difference in the growth of this strain in XOS and in the control was negligible.…”

Section: Oligosaccharides As Prebiotic Compoundsmentioning

confidence: 78%

“…The quantity of XOS present in these sources varies, depending on the source of xylan, and on the way of extraction (Aachary Ayyappan & Prapulla Siddalingaiya, ). In this sense, XOS from wheat straw xylan yielded 36% of XOS, and the ratio of X3/X3 increased with higher temperatures (Faryar et al, ). XOS from wheat bran xylan yielded 59% at 185°C and 10 min of residence time (Immerzeel et al, ).…”

Section: Oligosaccharides As Prebiotic Compoundsmentioning

confidence: 99%

Prebiotic compounds from agro‐industrial by‐products

2018

View full text Add to dashboard Cite

Prebiotics are nondigestible food components that have an impact on gut microbiota composition and activity, which in turn results in the improvement of health conditions. Nowadays, the production of prebiotics from agro‐industrial by‐products is under investigation. In this regard, polysaccharides are usually found in these sources and their potential use as prebiotics has been studied recently since these compounds act as substrates for the human gut microbiota, and they have the potential to modulate its composition through many mechanisms. Additionally, the use of agricultural by‐products is advantageous because it is a cheap and abundantly available material. This review focuses on the recent scientific literature regarding the prebiotic properties of polysaccharides from agro‐industrial by‐products. Practical applications Currently, the maintenance of gut homeostasis is a target for the improvement of human health. This review can broaden the perspective on the utilization of agro‐industrial by‐products that can compete in the market with the commercial ones or act as a source for new food ingredients.

show abstract

“…Cell pellets were collected by centrifugation, washed, and resuspended in binding buffer (BB, sodium phosphate 20 m m , NaCl 500 m m , pH 7.4), followed by disintegration by sonication, as described in Ref. . The cell extracts were centrifuged at 23 000 g , 4 °C, 20 min, and the resulting supernatants were directly loaded onto an immobilized metal ion affinity chromatography (IMAC) FF‐column (GE Healthcare, Uppsala, Sweden).…”

Section: Methodsmentioning

confidence: 99%

Three‐dimensional structures and functional studies of two GH43 arabinofuranosidases from Weissella sp. strain 142 and Lactobacillus brevis

et al. 2017

Self Cite

View full text Add to dashboard Cite

Arabinofuranosidases degrade arabinose-containing oligo and polysaccharides, releasing L-arabinose, which is a potentially useful sugar, shown to reduce glycemic response under certain conditions. Arabinofuranosidases (Arafs) are frequently found in GH43, one of the most common GHfamilies encoded in genomes in gut microbiota, and hence it is of interest to increase understanding of the function of these enzymes in species occurring in the gut. Here we have produced, characterized and solved the three-dimensional structures, at 1.9 and 2.0 A resolution respectively, of two homologous GH43 enzymes, classified under subfamily 26, from Lactobacillus brevis DSM1269 (LbAraf43) and Weissella strain 142 (WAraf43), respectively. The enzymes, with 74% sequence identity to each other, are composed of a single catalytic module with a b-propeller structure typical of GH43, and an active-site pocket with three identifiable subsites (À1, +1, and +2). According to size exclusion chromatography, native WAraf43 is a dimer, while LbAraf43 is a tetramer in solution. Both of them show activity with similar catalytic efficiency on 1,5-a-L-arabinooligosaccharides with a degree of polymerization (DP) of 2-3. Activity is restricted to substrates of low DP, and the reason for this is believed to be an extended loop at the entrance to the active site, creating interactions in the +2 subsite.

show abstract

Production of prebiotic xylooligosaccharides from alkaline extracted wheat straw using the K80R-variant of a thermostable alkali-tolerant xylanase

Cited by 75 publications

References 40 publications

Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes

Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes

Prebiotic compounds from agro‐industrial by‐products

Three‐dimensional structures and functional studies of two GH43 arabinofuranosidases from Weissella sp. strain 142 and Lactobacillus brevis

Contact Info

Product

Resources

About