Production of proteinase A bySaccharomyces cerevesiae in a cell-recycling fermentation system: experiments and computer simulations

Abstract

Overproduction of proteinase A by recombinant Saccharomyces cerevisiae was investigated by cultivations in a cell-recycling bioreactor. Membrane filtration was used to separate cells from the broth. Recycling ratios and dilution rates were varied and the effect on enzyme production was studied both experimentally and by computer simulations. Experiments and simulations showed that cell mass and product concentration were enhanced by high ratios of recycling. Additional simulations showed that the proteinase A concentration decreased drastically at high dilution rates and the optimal volumetric productivities were at high dilution rates just below washout and at high ratios of recycling. Cell-recycling fermentation gave much higher volumetric productivities and stable product concentrations in contrast to simple continuous fermentation.

Substrate Feeding Strategy Integrated with a Biomass Bayesian Estimator for a Biotechnological Process

“…Hybrid operation indicates the coexistence of continuous and discrete event dynamics during the process operation. This way, allowing to improve yields and production efficiencies with reduced operating costs and initial investments [2,3].…”