Production of recombinant human dipeptidyl peptidase IV from Sf9cells in microbial fermenters

Aytekin, Özlem Üstün; Gürhan, S. İsmet; Ohura, Kayoko; Imai, Teruko; Öngen, Gaye

doi:10.3906/biy-1503-9

Cited by 2 publications

References 41 publications

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Development of an animal-derived component-free medium for Spodoptera frugiperda (Sf9) cells using response surface methodology

et al. 2023

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Spodoptera frugiperda (Sf9) insect cells have been widely used for recombinant protein production. Currently, serum-free media for Sf9 cells are commercially available, but they mostly still contain animalderived components. This work aimed to develop an animal-derived component free medium for Sf9 cells. Chemically de ned lipid concentrate (CDLC), yeast extract (YE), and soytone (ST) were studied for serum substitutes using a central composite design (CCD). OSF9-ADCFM contained optimum concentrations of CDLC, YE and ST at 0.5% (v/v), 11.0 g/L, and 3.0 g/L, respectively. The mean viable cell concentration of 1.71 ± 0.14 x 10 5 cells/mL was obtained from 5 passages (P1-P5) in 25 cm 2 tissue culture ask. The use of both peptones after 10-kDa ultra ltration had a signi cant effect on Sf9 cells growth. Maximum cell concentrations and the speci c growth rates of cell adaptation in suspension culture were 1.79-2.17 x 10 6 cells/mL and 0.025-0.027 h − 1 , respectively. The results indicated that OSF9-ADCFM could be used for Sf9 culture in both static and suspension culture. Further study would be needed to verify the effect on protein production of this in-house animal-derived component-free medium.

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Development of an animal-derived component-free medium for Spodoptera frugiperda (Sf9) cells using response surface methodology

et al. 2023

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Development of an Animal-Derived Component-Free Medium for Spodoptera frugiperda (Sf9) Cells Using Response Surface Methodology

Saisud

Posung

Tuntigumthon

et al. 2023

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Spodoptera frugiperda (Sf9) insect cells have been widely used for recombinant protein production. Currently, serum-free media for Sf9 cells are commercially available, but they mostly still contain animal-derived components. This work aimed to develop an animal-derived component free medium for Sf9 cells. Chemically defined lipid concentrate (CDLC), yeast extract (YE), and soytone (ST) were studied for serum substitutes using a central composite design (CCD). OSF9-ADCFM contained optimum concentrations of CDLC, YE and ST at 0.5% (v/v), 11.0 g/L, and 3.0 g/L, respectively. The mean viable cell concentration of 1.71 ± 0.14 x 105 cells/mL was obtained from 5 passages (P1-P5) in 25 cm2 tissue culture flask. The use of both peptones after 10-kDa ultrafiltration had a significant effect on Sf9 cells growth. Maximum cell concentrations and the specific growth rates of cell adaptation in suspension culture were 1.79–2.17 x 106 cells/mL and 0.025–0.027 h− 1, respectively. The results indicated that OSF9-ADCFM could be used for Sf9 culture in both static and suspension culture. Further study would be needed to verify the effect on protein production of this in-house animal-derived component-free medium.

show abstract

Production of recombinant human dipeptidyl peptidase IV from Sf9cells in microbial fermenters

Cited by 2 publications

References 41 publications

Development of an animal-derived component-free medium for Spodoptera frugiperda (Sf9) cells using response surface methodology

Development of an animal-derived component-free medium for Spodoptera frugiperda (Sf9) cells using response surface methodology

Development of an Animal-Derived Component-Free Medium for Spodoptera frugiperda (Sf9) Cells Using Response Surface Methodology

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