Production of recombinant leptin and its effects on food intake in rainbow trout (Oncorhynchus mykiss)

Murashita, Koji; Uji, Susumu; Yamamoto, Takeshi; Rønnestad, Ivar; Kurokawa, Tadahide

doi:10.1016/j.cbpb.2008.04.007

Cited by 195 publications

(152 citation statements)

References 63 publications

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“…As LY294002 partially reverses this suppression, the importance of the Pi3k signaling pathway for the central action of Lep in the rainbow trout is further confirmed. The present data also agree with studies using peripheral Lep treatment of rainbow trout and Atlantic salmon, indicating that the anorexigenic action of Lep in salmonids is mediated through the Pomc pathway (Murashita et al 2008(Murashita et al , 2011. These data are also in agreement with data on frog Xenopus laevis, where ICV Lep administration increases pomc transcription (Cui et al 2014).…”

Section: Discussionsupporting

confidence: 91%

“…In the goldfish Carassius auratus that had been acclimated to a feeding schedule, ICV Lep administration did not affect npy expression in fed fish at 2 h post-feeding, but suppressed fasting-induced npy expression in unfed fish (Volkoff et al 2003). Peripheral Lep treatment also suppressed npy expression in 24-h fasted rainbow trout (Murashita et al 2008). In addition, Lep decreased npy expression in slices from rainbow trout hypothalamus without affecting pomc expression (Aguilar et al 2011).…”

Section: Discussionmentioning

confidence: 90%

“…While the Pomc genes (pomca1, pomca2, pomcb) are highly expressed in the rainbow trout hypothalamus (Leder & Silverstein 2006), and the Pomc cell bodies are found in the NLT region (Suzuki et al 1997, Tollemer et al 1999. Peripheral Lep treatment of rainbow trout has transiently reduced the hypothalamic npy expression levels, while stimulating the expression of pomca1 and pomca2 (Murashita et al 2008), indicating a similar neuropeptide mediation of anorexigenic effects of Lep in fish as in mammals. Intracerebroventricular (ICV) administration of Lep into rainbow trout has induced changes related to glucosensing in the hypothalamus and hindbrain to produce anorexia (Aguilar et al 2010).…”

Section: Introductionmentioning

confidence: 90%

“…It has been identified in many teleost species, including rainbow trout (Oncorhynchus mykiss), showing low protein sequence identity (13-25%) between fish and human Leps (Murashita et al 2008, Gorissen & Flik 2014. Based on lep gene expression data, the liver appears to be the primary Lep production site in teleosts (Londraville et al 2014), while mammalian LEP is primarily produced by the adipose tissue (Zhang et al 1994).…”

Section: Introductionmentioning

confidence: 99%

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Acute anorexigenic action of leptin in rainbow trout is mediated by the hypothalamic Pi3k pathway

Gong

Jönsson

Björnsson

2016

Journal of Molecular Endocrinology

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Leptin (Lep) is an anorexigenic hormone and regulates appetite-related neuropeptides in mammals. A number of neuropeptides have also been linked to appetite regulation in teleost fish, but Lep signaling activation and effects on appetite-regulating neurons are poorly elucidated in early vertebrates. This study uses cellular, tissue and organismal approaches to elucidate the acute, central Lep action in rainbow trout. The results demonstrate that Lep activates phosphorylation of protein kinase B (Akt) and signal transducer and activator of transcription 3 in rainbow trout hypothalamus-derived cells, and that the phosphatidylinositol-3-kinase (Pi3k) inhibitor LY294002 can suppress the Lep-induced Akt phosphorylation. Intracerebroventricular (ICV) Lep administration strongly suppresses food intake at the doses of 0.05 and 0.5 µg Lep fish −1 . At low dose, Lep stimulates hypothalamic transcription of anorexigenic cocaine-and amphetamineregulated transcript (Cart) and orexigenic neuropeptide Y. At high dose, Lep stimulates hypothalamic transcription of anorexigenic proopiomelanocortin (Pomc) A1, A2, and B, while coinjection with LY294002 reverses this upregulation. The data suggest that the anorexigenic action of Lep in rainbow trout is mediated through stimulation of the anorexigenic neuropeptides Pomc and Cart. Furthermore, ICV Lep treatment increases phosphor-Akt-immunoreactive cells in the nucleus lateralis tuberis, periventricular zone along infundibulum, and lateral recess surrounded by nucleus anterior tuberis, while LY294002 inhibits this effect. Lep receptor-immunoreactive cells are also predominant in these regions. These results demonstrate that Lep activates the Pi3k-Akt pathway in the lateral tuberal hypothalamus of rainbow trout for acute appetite regulation, indicating the conservation of anorexigenic Lep action in the mediobasal hypothalamus.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Acute anorexigenic action of leptin in rainbow trout is mediated by the hypothalamic Pi3k pathway

Gong

Jönsson

Björnsson

2016

Journal of Molecular Endocrinology

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“…Leptins also were identified in non-mammalians, including birds [3][4][5], reptiles [2], amphibians [6][7][8], and teleosts, the later generally possess duplicated leptins [8][9][10][11][12][13][14][15]. Leptin has been found to be responsible for the regulation of body weight and energy homeostasis [16,17], and it also is involved in regulating appetite, reproduction [18], the immune system [19], bone formation [20], angiogenesis [21], and stress response [22,23].…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, characterization and evolutionary analysis of leptin gene in Chinese giant salamander, Andrias davidianus

Tian

Meng

et al. 2017

Open Life Sciences

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Abstract:Leptin is an important hormone possessing diverse physiological roles in mammals and teleosts. However, it has been characterized only in a few amphibian species, and its evolutions are still under debate. Here, the full length of the leptin (Adlep) cDNA of Chinese giant salamander (Andrias davidianus), an early diverging amphibian species, is characterized and according to the results of the primary sequence analysis, tertiary structure reconstruction and phylogenetic analysis is confirmed to be an ortholog of mammalian leptin. An intron was identified between the coding exons of A. davidianus leptin, which indicated that the leptin is present in the salamander genome and contains a conserved gene structure in vertebrates. Adlep is widely distributed but expression levels vary among different tissues, with highest expression levels in the muscle. Additionally, the leptin receptor and other genes were mapped to three known leptin signaling pathways, suggesting that the leptin signaling pathways are present in A. davidianus. Phylogenetic topology of leptins are consistent with the generally accepted evolutionary relationships of vertebrates, and multiple leptin members found in teleosts seem to be obtained through a Cluopeocephala-specific gene duplication event. Our results will lay a foundation for further investigations into the physiological roles of leptin in A. davidianus.

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Appetite and Feed Intake

Jobling

Alanärä

Noble

et al. 2012

Aquaculture and Behavior

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This chapter first describes changes in appetite that occur over different time scales in wild fish and the physiological mechanisms that generate these changes. It then gives an account of how food intake and appetite change as young fish develop and mature and the effects of genetic variation and differential experience on these processes. The costs of sustaining a high rate of food intake are then described, as is the way in which these are balanced against the obvious benefits of gaining a good supply of nutrients. The benefits gained by regular appetite cycles are also considered. The implications of natural appetite patterns for fish culture are then discussed, including problems arising from underfeeding and overfeeding and those resulting from failure to match feed delivery to current appetite. Underfeeding causes slow, uneven growth and high levels of aggression, as well as stress and mortality, while overfeeding may result in inefficient production and adverse environmental impact. Potential solutions to such problems are discussed, including the use of on-demand feeding systems that match delivery to appetite. The effects of domestication and captive rearing on feed intake are then considered, together with possible ways of mitigating such effects in fish that are cultured for release.

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Production of recombinant leptin and its effects on food intake in rainbow trout (Oncorhynchus mykiss)

Cited by 195 publications

References 63 publications

Acute anorexigenic action of leptin in rainbow trout is mediated by the hypothalamic Pi3k pathway

Acute anorexigenic action of leptin in rainbow trout is mediated by the hypothalamic Pi3k pathway

Molecular cloning, characterization and evolutionary analysis of leptin gene in Chinese giant salamander, Andrias davidianus

Appetite and Feed Intake

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