Production of Rifamycin using Nocardia mediterranea (ATCC 13865)

Abstract

10, 20, 30, 40, 50, 60, 70, up to 120 minute the maximum (581µgml -1 ) production of rifamycin was found developed S-10 strain for maximum production of rifamycin antibiotics (optimum time 15 minute) for further enhancement of rifamycin various parameter like age and size inoculum, pH of the culture medium, various carbohydrate, various amino acids, temperature, various Carbone sources, aeration were optimized. The maximum rifamycin conc. of 570 µgml -1 at 4.50 inoculums % with the age of 48 hrs. Inoculum was given even maximum rifamycin production with various age of inoculums (24, 36, 48, 60, 72 hrs.) with corresponding inoculums % (1.5, 2.5, 3.5, 4.5) in culture media, the optimum rifamycin 485 and 480 µgml -1 of rifamycin was producing M-6 and M-8 respectively medium amongst M-1, 2, 3, 4, 5, 6, 7, 8, 9 and M-10.