10, 20, 30, 40, 50, 60, 70, up to 120 minute the maximum (581µgml -1 ) production of rifamycin was found developed S-10 strain for maximum production of rifamycin antibiotics (optimum time 15 minute) for further enhancement of rifamycin various parameter like age and size inoculum, pH of the culture medium, various carbohydrate, various amino acids, temperature, various Carbone sources, aeration were optimized. The maximum rifamycin conc. of 570 µgml -1 at 4.50 inoculums % with the age of 48 hrs. Inoculum was given even maximum rifamycin production with various age of inoculums (24, 36, 48, 60, 72 hrs.) with corresponding inoculums % (1.5, 2.5, 3.5, 4.5) in culture media, the optimum rifamycin 485 and 480 µgml -1 of rifamycin was producing M-6 and M-8 respectively medium amongst M-1, 2, 3, 4, 5, 6, 7, 8, 9 and M-10.

The present study was carried out to evaluate the optimum Production of Rifamycin B by using fed-batch mode in the shake flask at the defenit level of different agitation and aeration rate, amongst different carbohydrate in the form of monoshacride. Amongst the various carbohydrate (Arabinose, Fructose, Galactose, Glucose, Glycerol, Maltose, Mannitol, Sorbitol, and Sucrose) sources maximum, the rifamycin (578 µgml-1) with 10% glucose level was found as compared to control which was (138 ± 2.70µgml-1).The environmental factors such as incubation temperature. The optimum temperature for maximum antibiotic production (525µgml-1) was found to be 29°C, initial pH, aeration and agitation rates were also optimized. The optimum rifamycin yield (950 to 1100µgml-1) was obtained in 1.0 L fermenter with working volume of 1.5 L at 28 °C with initial pH of 6.2 to 6.5. The agitation and aeration were 300 rpm and 0.5 LL-1 min-1 respectively.