Production of <scp>D</scp>‐Lactic Acid by Bacterial Fermentation of Rice Starch

Fukushima, Kazuki; Sogo, Kazuaki; Miura, Shigenobu; Kimura, Yoshiharu

doi:10.1002/mabi.200400080

Cited by 101 publications

(52 citation statements)

References 14 publications

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“…Therefore, many researchers have examined the direct production of lactic acid from starchy materials by using wild amylolytic LAB (ALAB) (6,24,25) or genetically modified amylase-producing LAB (15,16). Although D-lactic acid has been produced by fermentation from pretreated substrates such as rice starch (5) and by simultaneous saccharification and fermentation from cellulose (23), there have been no reports on the direct production of D-lactic acid from starchy materials. This is due to a lack of D-lactic acid-producing ALAB and difficulties in gene manipulation of D-lactic acid-producing LAB, such as Lactobacillus delbrueckii (22).…”

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confidence: 99%

Efficient Production of Optically Pure d -Lactic Acid from Raw Corn Starch by Using a Genetically Modified l -Lactate Dehydrogenase Gene-Deficient and α-Amylase-Secreting Lactobacillus plantarum Strain

Okano¹,

Zhang²,

Shinkawa³

et al. 2009

Appl Environ Microbiol

102

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In order to achieve direct and efficient fermentation of optically pure D-lactic acid from raw corn starch, we constructed L-lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum and introduced a plasmid encoding Streptococcus bovis 148 ␣-amylase (AmyA). The resulting strain produced only D-lactic acid from glucose and successfully expressed amyA. With the aid of secreting AmyA, direct D-lactic acid fermentation from raw corn starch was accomplished. After 48 h of fermentation, 73.2 g/liter of lactic acid was produced with a high yield (0.85 g per g of consumed sugar) and an optical purity of 99.6%. Moreover, a strain replacing the ldhL1 gene with an amyA-secreting expression cassette was constructed. Using this strain, direct D-lactic acid fermentation from raw corn starch was accomplished in the absence of selective pressure by antibiotics. This is the first report of direct D-lactic acid fermentation from raw starch.

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confidence: 99%

Efficient Production of Optically Pure d -Lactic Acid from Raw Corn Starch by Using a Genetically Modified l -Lactate Dehydrogenase Gene-Deficient and α-Amylase-Secreting Lactobacillus plantarum Strain

Okano¹,

Zhang²,

Shinkawa³

et al. 2009

Appl Environ Microbiol

102

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“…It is interesting to note that the sc-crystal shows a melting temperature of 220-230 C, which is about 50-60 C higher than those for the homo-crystals. Recently, Kimura et al 15,16 found bacteria which produces Dlactic acid from glucose. This finding may allow to produce D-lactic acid more easily at a lower coast and enhance the application of sc-poly(lactic acid).…”

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confidence: 99%

Development of Oriented Morphology and Mechanical Properties upon Drawing of Stereo-Complex of Poly(L-lactic acid) and Poly(D-lactic acid) by Solid-State Coextrusion

Sawai

Tamada

Kanamoto

2007

Polym J

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ABSTRACT:A semicrystalline film consisting of only stereo-complex (sc) crystals was prepared by casting from a solution of an equimolar blend of poly(L-lactic acid) (PLLA) and poly(D-lactic acid) (PDLA). The film was uniaxially drawn by solid-state coextrusion, and the development of oriented morphology and mechanical properties was investigated by DSC, WAXD, dynamic mechanical analyses and tensile tests. The ductility increased with extrusion temperature (T ext ), reached a maximum at a T ext of 200 C, and at yet higher T ext 's it rapidly decreased. The maximum tensile modulus and strength of 9.5 GPa and 410 MPa, respectively, were achieved for a highly drawn sc-film prepared by coextrusion at a T ext of 200 C and EDR of 16. Such a drawn film consisted of highly oriented sc-crystals and had a crystallinity of c ¼ 59 wt % and a high chain orientation function of f c ¼ 0:993. The oriented sc-films exhibited remarkably enhanced heat-resistance compared to the oriented films of PLLA homo-polymer. The dynamic storage modulus of a sc-film with an EDR of 16, measured at 11 Hz, was 9.5 GPa at room temperature and 2.7 GPa even at 200 C, 20-25 C above the melting temperatures of -and -form homo-crystals. The high thermal stability of oriented sc-films was primarily ascribed to the high melting temperature and weak -relaxation intensity for the sc-films compared to the PLLA films. No existence of a crystalline relaxation in the sc-films also contributed to improve their thermal stability. Poly(L-lactic acid) (PLLA) attracts considerable interest from both fundamental and industrial viewpoints because it can be synthesized from renewable resources, such as corn and potato.1-3 Since PLLA is biodegradable and biocompatible, this polymer has been used for biomedical applications.4-6 Because PLLA has fairly good mechanical properties with a high melting temperature (T m ¼ 170{180 C), its application for more general purposes as an environmentally friendly and economically feasible polymer has been evaluated. [7][8][9] PLLA commonly crystallizes into two crystal modifications; i.e., the -and the -forms, 10-12 both of which have a melting temperature around 170-180 C. It has been reported that an equimolar blend of PLLA and poly(D-lactic acid) (PDLA) forms a mixture of stereo-complex crystals and -form homocrystals when it was crystallized from solutions and melt, depending on the crystallization conditions. 13,14 Homo-crystals, such as -form and -form crystals consist of only PLLA or PDLA molecules, whereas the sc-crystal is composed of PLLA and PDLA chains with opposite helical hand packed side by side. It is interesting to note that the sc-crystal shows a melting temperature of 220-230 C, which is about 50-60 C higher than those for the homo-crystals. Recently, Kimura et al. 15,16 found bacteria which produces Dlactic acid from glucose. This finding may allow to produce D-lactic acid more easily at a lower coast and enhance the application of sc-poly(lactic acid).It has been reported that when an equimolar blend of PLLA and ...

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“…Lactobacillus) inulinus (5). Because of its high-optical-purity D-lactic acid-producing capability, Sporolactobacillus inulinus has been suggested to be a better producer of D-lactic acid (2,12). Sporolactobacillus inulinus CASD, isolated by our group (10), can produce high concentrations of D-lactic acid (207 g/liter) with very high optical purity at 99.3%, which is the highest production titer reported to date (9).…”

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confidence: 84%

Draft Genome Sequence of Sporolactobacillus inulinus Strain CASD, an Efficient D-Lactic Acid-Producing Bacterium with High-Concentration Lactate Tolerance Capability

Yu¹,

Su²,

Wang³

et al. 2011

Journal of Bacteriology

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Sporolactobacillus inulinus CASD is an efficient D-lactic acid producer with high optical purity. Here we report for the first time the draft genome sequence of S. inulinus (2,930,096 bp). The large number of annotated two-component system genes makes it possible to explore the mechanism of extraordinary lactate tolerance of S. inulinus CASD.Lactic acid, the most important hydroxycarboxylic acid, is regarded as a feedstock for the green chemistry of the future. Besides its versatile applications in the food, pharmaceutical, textile, and leather industries, lactic acid is also a building block for biodegradable plastic, mainly poly(L-lactic acid), which has gained substantial attention in the new wave of industrial biotechnology (7, 4). The development of a stereocomplex of poly(L-lactic acid) and poly(D-lactic acid) is considered an advance in poly(lactic acid) modification, in which the melting point of this stereocomplex could reach the same temperature as that of other petroleum-based polymers (3). Therefore, the potential utilization of D-lactic acid has attracted increased attention to its production by using sustainable bioresource materials (8,12).Sporolactobacillus is a genus of anaerobic endosporeforming, Gram-positive, motile, rod-shaped lactic acid bacteria. Sporolactobacillus inulinus, the type species of the genus, was originally named Sporolactobacillus (subgen.

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Production of D‐Lactic Acid by Bacterial Fermentation of Rice Starch

Cited by 101 publications

References 14 publications

Efficient Production of Optically Pure d -Lactic Acid from Raw Corn Starch by Using a Genetically Modified l -Lactate Dehydrogenase Gene-Deficient and α-Amylase-Secreting Lactobacillus plantarum Strain

Efficient Production of Optically Pure d -Lactic Acid from Raw Corn Starch by Using a Genetically Modified l -Lactate Dehydrogenase Gene-Deficient and α-Amylase-Secreting Lactobacillus plantarum Strain

Development of Oriented Morphology and Mechanical Properties upon Drawing of Stereo-Complex of Poly(L-lactic acid) and Poly(D-lactic acid) by Solid-State Coextrusion

Draft Genome Sequence of Sporolactobacillus inulinus Strain CASD, an Efficient D-Lactic Acid-Producing Bacterium with High-Concentration Lactate Tolerance Capability

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