Production ofl-Leucine by a Mutant ofBrevibacterium lactofermentum2256

Assignment of the three methionyl carbonyl carbon resonances in Streptomyces subtilisin inhibitor by a carbon-13 and nitrogen-15 double-labeling technique. A new strategy for structural studies of proteins in solution

“…(Aldrich) and sodium [13C]cyanide (92.2% 13C), according to the method of Holland & Nayler (1952). l-[15N] Valine was prepared by microbial fermentation with Brevibacterium lactofermentum (Tsuchida et al, 1974). [15N]Ammonium sulfate (Hikari Kogyo, 98.4% 15N) was used in lieu of normal [14N]ammonium sulfate as the sole nitrogen source. No appreciable isotopic dilution was detected during the fermentation.…”

Fermentative Production ofl-Leucine with Auxotrophic Mutants ofCorynebaeterium glutamicum

“…7~8.4 mg/ml the values given by the parent strain. Moreover, 10 out of the 47 mutants produced more than 11 mg/ml of L-luecine (Tables VII and 10 II~16.0 6.6> 6 …”

Properties of Revertants Appearing inl-Leucine Fermentation Culture Broth

“…Accumulation of L-Ieucine in the culture broth has been reported in Salmonella typhimurium,l) Serratia marcescens,2) Corynebacterium glutamicum 3 ,4) and Brevibacterium lactofermentum. 5 ) The stability of a strain is one of the most important factors along with its productivity for microbial production especially on an industrial scale. There have been a great number of reports on amino acids producing mutants, but very few of them refer to the stability or stabilization of the mutants.…”