Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation

Murthy, Hosakatte Niranjana; Lee, Eunjung; Paek, Kee-Yoeup

doi:10.1007/s11240-014-0467-7

Cited by 554 publications

(348 citation statements)

References 133 publications

Supporting

Mentioning

317

Contrasting

Unclassified

Order By: Relevance

“…Modifications of phytohormone composition offer wide possibilities of improving cell culture productivity with respect to therapeutically relevant constituents (Murthy et al 2014). However, difficulties can arise from often opposite effects of auxins and cytokinins towards secondary metabolism.…”

Section: Discussionmentioning

confidence: 99%

Effect of plant growth regulators on the accumulation of indolizidine alkaloids in Securinega suffruticosa callus cultures

Raj

Kokotkiewicz

Dryś

et al. 2015

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

In the presented work, plant growth regulators (PGRs) were tested for their influence on biomass growth and accumulation of therapeutically-relevant indolizidine alkaloids in Securinega suffruticosa (Phyllanthaceae) callus cultures. The study included 9 auxins [2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid, indole-3-acetic acid (IAA), 4-chlorophenoxyacetic acid, indole-3-butyric acid, 4-amino-3,5,6-trichloropicolinic acid, b-naphthoxyacetic acid, 1-naphthaleneacetic acid, indole-3-propionic acid, one polar auxin transport inhibitor (2,3,5-triiodobenzoic acid) and 7 cytokinins [2-isopentenyladenine, 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea, N-(2-chloro-4-pyridinyl)-N 0 -phenylurea, diphenylurea, zeatin, kinetin (KIN), 6-benzylaminopurine]. The respective PGRs were applied at 0.5 and 5.0 mg l -1 , together with 5.0 or 0.5 mg l -1 KIN or 2,4-D (for auxins and cytokinins, respectively). The calli subjected to different phytohormone combinations (39 modifications in total) were evaluated for growth and alkaloid content, and the results tested for statistical differences. The highest concentrations of securinine (1.73 mg g -1 DW) and allosecurinine (3.11 mg g -1 DW) were recorded in the callus grown in the presence of 0.5 mg L -1 IAA and 5.0 mg L -1 KIN. To the best of the authors' knowledge, this is the first such comprehensive report concerning the influence of PGRs on alkaloid accumulation in plant in vitro cultures.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of plant growth regulators on the accumulation of indolizidine alkaloids in Securinega suffruticosa callus cultures

Raj

Kokotkiewicz

Dryś

et al. 2015

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…However, these authors mention that a higher content of secondary metabolites in the explant is common, since it still has its differentiated tissues, which favors the secondary metabolism (Santos-Filho et al, 2014a;Nicioli et al, 2010). Thus, the cultivation in vitro allows the use of different strategies to change the metabolite production by cells (Murthy et al, 2014). In this context, glutamine may act both as precursor and as elicitor of secondary metabolism (Matkowski, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…With this scenario in sight, plant tissue culture techniques comes as an alternative to get bioactive compounds (Karuppusamy, 2009). Consequently, studies have been carried out in order to enhance the synthesis and/or the accumulation of metabolites in plant cells and organs grown in vitro, such as optimization of culture medium and environment and the use of elicitation strategies (Murthy et al, 2014). An alternative is the use of organic forms of nitrogen, i.e., yeast extract, hydrolyzed casein and amino acids aiming at increasing cell metabolism in plant tissues (Parast et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Proteic and phenolics compounds contents in Bacupari callus cultured with glutamine and nitrogen sources

et al. 2017

View full text Add to dashboard Cite

In this study was evaluated the influence of glutamine supplementation on the endogenous content of amino acids, proteins, total phenolics, flavonoids and proanthocyanidins in Bacupari callus. The explants were inoculated in MS medium, MS with half concentration of the nitrogen salts (MS½) and nitrogen-free MS, supplemented with glutamine (5, 10, 30 and 60mM) named as Gln5, Gln10, Gln30 and Gln60. Amino acids and proteins were analyzed after 20, 80 and 140 days and the secondary metabolites on the 140 th day. There was no difference in the amino acids on the 20 th day. On the 80 th day the treatments MS and MS½ presented the lowest levels. On the 140 th day MS and MS½ presented the lowest amino acid concentration and Gln10 the highest. Concerning proteins, there was difference only on the 140 th day, being the highest concentrations observed in Gln5, and the lowest in MS½ treatment. Total phenolics content was higher in the treatment Gln60 and lowest in MS. Treatments Gln5, Gln10, Gln30 and MS½ were statistically equal. For flavonoids, the highest values occurred in the treatments Gln30, Gln60 and MS½ and the lowest in Gln5, Gln10 and MS. Similarly, for the proanthocyanidins the highest concentrations were observed in treatment Gln60 and the lowest in Gln5 and MS. In conclusion, the treatment with 60mM of glutamine favors the protein accumulation and production of secondary metabolites in Bacupari callus.Keywords: glutamine, phenols, flavonoids, proanthocyanidins, amino acids. Conteúdo de proteínas e compostos fenólicos em calos de Bacupari cultivados com glutamina e fontes de nitrogênio ResumoNesse estudo foi avaliado o efeito da suplementação com glutamina no conteúdo endógeno de aminoácidos, proteínas, fenólicos totais, flavonoides e proantocianidinas em calos de Bacupari. Os explantes foram inoculados em meio MS, meio MS com metade da concentração de dos sais de nitrogênio (MS½) e meio MS sem nitrogênio suplementado com glutamina (5, 10, 30 e 60mM) denominados como Gln5, Gln10, Gln30 e Gln60. Os aminoácidos e as proteínas foram analisados após 20, 80 e 140 dias e os metabólitos secundários no 140° dia. Não houve diferença nos aminoácidos no 20° dia. No 80° dia os tratamentos MS e MS½ apresentaram os menores níveis. No 140° dia, MS e MS½ apresentaram as menores concentrações de aminoácidos e o Gln10 as maiores. A respeito das proteínas, houve diferença apenas no 140° dia, sendo as maiores concentrações observadas nos tratamentos Gln, e as menores no MS½. O conteúdo de fenólicos totais foi maior no tratamento Gln60 e menor no MS. Os tratamentos Gln5, Gln10, Gln30 e MS½ foram estatisticamente iguais. Para os flavonóides, os maiores valores ocorreram nos tratamentos Gln30, Gln60 e MS½ e os menores no Gln5, Gln10 e MS. Da mesma forma, para as proantocianidinas, as maiores concentrações foram observadas no tratamento Gln60 os menores no Gln5 e MS. Em conclusão, o tratamento com 60 mM de glutamina favorece o acúmulo de proteínas e a produção de metabólitos secundários em calos de Bacupari.

show abstract

“…In vitro cultures of higher plants can be regarded as an alternative, renewable source of high-value secondary metabolites (Murthy et al 2014), with ginsenosides (Kochan et al 2014;Liu et al 2014), hypericin (Wu et al 2014) and the anticancer lignan podophyllotoxin (Rajesh et al 2014) being some of the well-known examples. As compared to field cultivation, in vitro techniques offer the possibility of continuous production of large amounts of chemically uniform biomass independently of wild resources and environmental factors (Murthy et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…As compared to field cultivation, in vitro techniques offer the possibility of continuous production of large amounts of chemically uniform biomass independently of wild resources and environmental factors (Murthy et al 2014). As far as essential oils are concerned, in vitro cultivation also gives the possibility to obtain novel compounds, previously not reported in intact plants (Gounaris 2010;Gonçalves and Romano 2013;Marchev et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Production of essential oils from in vitro cultures of Caryopteris species and comparison of their concentrations with in vivo plants

et al. 2015

View full text Add to dashboard Cite

The chemical composition of hydrodistilled essential oils obtained from aerial parts and roots of selected Caryopteris ('bluebeard') species (C. incana, C. mongolica, Caryopteris 9 clandonensis), as well as the newly established in vitro shoot and adventitious root cultures of the above plants, was analyzed by gas chromatography-mass spectrometry. Essential oil content and composition differed significantly depending on the type of plant material analyzed. Adventitious roots were characterized by the highest essential oil yield, reaching 1.8 % V/DW in Caryopteris 9 clandonensis. Limonene and cedrol were the main components of the essential oil derived from aerial parts of the intact plants (11.9-16.0 and 10.7-10.9 %, respectively), whereas the volatile fractions of the in vivo roots of all species contained large amounts of 3,5-bis(1,1-dimethyl)-phenol (12.9-26.2 %). 1,8-cineole, absent in the intact plant materials, was the dominating volatile constituent of the essential oils obtained from in vitro shoots (24.8-34.2 %). The volatile oil derived from adventitious root cultures consisted primarily of 1-octen-3-ol (19.7-31.5 %) and medicinally relevant diterpenoids: abietatriene and trans-totarol, which were accumulated in considerable quantities, especially in the adventitious roots of C. clandonensis (21.6 and 29.2 %, respectively).

show abstract

Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation

Cited by 554 publications

References 133 publications

Effect of plant growth regulators on the accumulation of indolizidine alkaloids in Securinega suffruticosa callus cultures

Effect of plant growth regulators on the accumulation of indolizidine alkaloids in Securinega suffruticosa callus cultures

Proteic and phenolics compounds contents in Bacupari callus cultured with glutamine and nitrogen sources

Production of essential oils from in vitro cultures of Caryopteris species and comparison of their concentrations with in vivo plants

Contact Info

Product

Resources

About