Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon

Fujita, Yasuhiro; Hara, Yasuhiro; Ogino, Takao; Suga, Chuzo

doi:10.1007/bf00269272

Cited by 161 publications

(71 citation statements)

References 8 publications

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“…Study of M9 medium and methyl jasmonate effect on shikonin derivatives productivity M9 medium, developed by Fujita et al (1981) and successfully used for the enhancement of shikonin production in suspension culture of L. erythrorhizon, was employed to increase the production of shikonin derivatives in hairy root cultures of L. canescens. Considering the time course study of dry weight increase and of ACS and IBS accumulation it could be inferred that these two processes coincide and the highest shikonin derivative concentrations were determined during the linear growth phase between culture days 25 and 32.…”

Section: Resultsmentioning

confidence: 99%

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Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

Sykłowska-Baranek

Pietrosiuk

Gawron

et al. 2011

Plant Cell Tiss Organ Cult

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The effects of medium exchange and methyl jasmonate addition on growth and production of shikonin and its derivatives acetylshikonin and isobutyrylshikonin in hairy root cultures of Lithospermum canescens were investigated. Responses varied depending on the transgenic line and stage of growth at which these lines were subjected to treatment. Shikonin itself was not detected, irrespective of the transgenic line and culture treatment used. A eightfold increase in acetylshikonin and isobutyrylshikonin accumulation was achieved when 32-day-old transgenic roots of Lc1D line were transferred from LS to M9 medium for the subsequent 3 weeks of culture. Methyl jasmonate exerted a detrimental effect on red naphthoquinones production. The extracts obtained from roots cultivated in M9 medium for 3 weeks were subjected to a cytotoxicity assay and displayed cytotoxic activity against human promyelocytic leukemia cells (HL-60) at the dose of 4 lg ml -1 .

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Section: Resultsmentioning

confidence: 99%

“…To improve shikonin derivatives accumulation, the transgenic roots were moved from LS medium to M9 medium and cultivated in 250 ml Erlenmeyer flasks containing 50 ml of liquid M9 medium (Fujita et al 1981). The culture conditions were as described above.…”

Section: Treatment For Enhancement Of Shikonin Derivatives Productionmentioning

confidence: 99%

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

Sykłowska-Baranek

Pietrosiuk

Gawron

et al. 2011

Plant Cell Tiss Organ Cult

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“…The callus induced from leaves and stems showed best growth rates, up to 300% in 3 weeks, and friability when sub-cultured on LS medium supplemented with 2,4-D (10 −6 M), kinetin (10 −5 M), and sucrose (5%). For shikalkin production, calli were transferred onto White medium (Fujita et al, 1981a). The concentration of copper, as an elicitor, was increased to 0.3 g L −1 (Fujita et al, 1981b) and sucrose was reduced to 2.5% to establish suitable carbon/nitrogen ratio for the secondary metabolites production (Srinivasan & Ryu, 1993).…”

Section: Resultsmentioning

confidence: 99%

“…For shikalkin production, calli were transferred onto White medium (Fujita et al, 1981a). The concentration of copper, as an elicitor, was increased to 0.3 g L −1 (Fujita et al, 1981b) and sucrose was reduced to 2.5% to establish suitable carbon/nitrogen ratio for the secondary metabolites production (Srinivasan & Ryu, 1993). Calli were then divided into two groups.…”

Section: Resultsmentioning

confidence: 99%

Lithospermum officinale callus produces shikalkin

et al. 2006

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To study biosynthetic abilities of Lithospermum officinale, callus formation from young leaves and stems of the plant was induced on Linsmaier-Skoog medium supplemented with 2,4-D (10 −6 M) and kinetin (10 −5 M). Maintaining the calli on this medium resulted in polyphenolic compounds production. Their transfer onto White medium containing IAA (10 −7 M) and kinetin (10 −5 M) resulted in the production of a red naphthoquinonic pigment named shikalkin. Shikalkin production from callus cultures was suppressed on the White medium containing NAA instead of IAA. This observation indicates that both shikalkin and polyphenolic acids biosynthetic pathways exist in the L. officinale callus cells and a regulatory system counterbalances the ratio of shikalkin to polyphenolic acids.

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“…On sait aussi que la source d'azote affecte la formation des métabolites secondaires [8,30]. Dans notre étude, le nitrate de potassium (3 g L -1 ) et le sulfate d'ammonium (134 mg L -1 ) constituent les sources d'azote retenues, mais il serait peut être nécessaire de vérifier la production de b-thujaplicine dans les cellules de Thuja plicata en fonction de la valeur du rapport NO -3 /NH + 4 .…”

Section: Discussionunclassified

Biosynth�se de tropolones dans les cals et les suspensions cellulaires � partir d'�bauches foliaires de plantules de Thuja plicata Donn

Haluk¹,

Roussel-Bousta²

2003

Ann. For. Sci.

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Résumé -Nous avons développé des cultures cellulaires de Thuja plicata Donn à partir de cals provenant d'ébauches foliaires de plantules cultivés sur milieu de Gamborg B5 enrichi en saccharose ou en glucose, complémenté de benzylaminopurine (BAP) et d'acide 1-naphtalène acétique (NAH) comme régulateurs de croissance. La synthèse de novo de la b-thujaplicine, tropolone naturelle majoritaire dans de nombreuses espèces de Cupressacées, est accélérée en présence d'éliciteur du type extrait de levure. L'extraction des tropolones intracellulaires est réalisée par broyage des cellules en présence d'acétate d'éthyle. La culture des cals en présence d'éliciteur et de saccharose à 2 % conduit à un rendement plus intéressant en tropolones (6,5 mg g -1 de cal frais) comparativement à celui sans éliciteur (1,75 mg). L'utilisation de glucose à 2 % améliore également les rendements en tropolones (3,15 mg sans éliciteur et 8,1 mg avec éliciteur par g de cal frais). Les cultures de suspensions cellulaires de Thuja plicata réalisées à partir de cals montrent que la vitesse d'agitation de l'agitateur orbitalaire influence la biosynthèse des tropolones (maximum de 5,1 mg g -1 de cellules fraîches à 120 rpm au bout de 14 jours de croissance contre 2 mg à 80 et 180 rpm). red cedar / tropolones / b-thujaplicine / éliciteurs / cals / suspensions cellulairesAbstract -Biosynthesis of tropolones from callus and suspension-cultured cells initiated from young seedlings of Thuja plicata Donn. Thuja plicata callus and cell cultures have been cultured in Gamborg B5 medium supplemented with saccharose or glucose, 1-naphtalene acetic acid (NAA) and benzylaminopurine (BAP) as growth regulators. The addition of elicitor as yeast extract to callus culture of Thuja plicata leads to an increase in the production of tropolones, typical heartwood constituents of Cupressus species. Calli cultured in a medium supplemented with saccharose 2% and yeast extract accumulate tropolones in about 6.5 mg g -1 cell fresh weight, which is easily extracted from callus culture with ethylacetate. Without elicitation, the content of tropolones was only 1.75 mg g -1 cell fresh weight. In a medium supplemented with glucose, the content of tropolone increases (8.1 mg with elicitation and 3.15 mg without elicitation). Cell cultures obtained from calli show an effect of the rotary shaker speed on the tropolones production (5.1 mg g -1 at 120 rpm agitation and only 2 mg at 80 or 180 rpm). red cedar / tropolones / b-thujaplicine / elicitors / callus / cell culture

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Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon

Cited by 161 publications

References 8 publications

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

Lithospermum officinale callus produces shikalkin

Biosynth�se de tropolones dans les cals et les suspensions cellulaires � partir d'�bauches foliaires de plantules de Thuja plicata Donn

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