Production of single-round infectious chimeric flaviviruses with DNA-based Japanese encephalitis virus replicon

Suzuki, Ritsuro; Ishikawa, Takahiro; Konishi, Eiji; Matsuda, Mami; Watashi, Koichi; Aizaki, Hideki; Takasaki, Tomohiko; Wakita, Takaji

doi:10.1099/vir.0.058008-0

Cited by 40 publications

(38 citation statements)

References 30 publications

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“…These results suggest that the ESCRT pathway does not play an essential role in genomic RNA replication. When viral structural proteins were supplied in trans into the sub-genomic replicon cells, single-round infectious particles (SRIPs) were released from the cells (Suzuki et al, 2014). The infectivity of SRIPs produced from TSG101-, CHMP2/3-, or CHMP4depleted cells was significantly decreased relative to control cells ( Figure 2B).…”

Section: The Escrt Pathway Is Essential For the Late Stage Of Viral Imentioning

confidence: 99%

Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum

et al. 2016

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Flavivirus infection induces endoplasmic reticulum (ER) membrane rearrangements to generate a compartment for replication of the viral genome and assembly of viral particles. Using quantitative mass spectrometry, we identified several ESCRT (endosomal sorting complex required for transport) proteins that are recruited to sites of virus replication on the ER. Systematic small interfering RNA (siRNA) screening revealed that release of both dengue virus and Japanese encephalitis virus was dramatically decreased by single depletion of TSG101 or co-depletion of specific combinations of ESCRT-III proteins, resulting in ≥1,000-fold titer reductions. By contrast, release was unaffected by depletion of some core ESCRTs, including VPS4. Reintroduction of ESCRT proteins to siRNA-depleted cells revealed interactions among ESCRT proteins that are crucial for flavivirus budding. Electron-microscopy studies revealed that the CHMP2 and CHMP4 proteins function directly in membrane deformation at the ER. Thus, a unique and specific subset of ESCRT contributes to ER membrane biogenesis during flavivirus infection.

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Section: The Escrt Pathway Is Essential For the Late Stage Of Viral Imentioning

confidence: 99%

Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum

et al. 2016

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“…Flaviviruses replicons, characterized by their high efficiency in expressing heterologous genes without producing infectious progeny virus, are useful tools for understanding the replication of viruses and exploring antiviral screening, and can also be applied as a potential expression system to be an antivirus vaccine candidate (Cao et al, 2011; Suzuki et al, 2014). Replicons vaccines have several advantages over inactivated vaccines and many subunit vaccines.…”

Section: Discussionmentioning

confidence: 99%

A highly pathogenic porcine reproductive and respiratory syndrome virus candidate vaccine based on Japanese encephalitis virus replicon system

Chen

Huang

et al. 2017

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In the swine industry, porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease which causes heavy economic losses worldwide. Effective prevention and disease control is an important issue. In this study, we described the construction of a Japanese encephalitis virus (JEV) DNA-based replicon with a cytomegalovirus (CMV) promoter based on the genome of Japanese encephalitis live vaccine virus SA14-14-2, which is capable of offering a potentially novel way to develop and produce vaccines against a major pathogen of global health. This JEV DNA-based replicon contains a large deletion in the structural genes (C-prM-E). A PRRSV GP5/M was inserted into the deletion position of JEV DNA-based replicons to develop a chimeric replicon vaccine candidate for PRRSV. The results showed that BALB/c mice models with the replicon vaccines pJEV-REP-G-2A-M-IRES and pJEV-REP-G-2A-M stimulated antibody responses and induced a cellular immune response. Analysis of ELSA data showed that vaccination with the replicon vaccine expressing GP5/M induced a better antibodies response than traditional DNA vaccines. Therefore, the results suggested that this ectopic expression system based on JEV DNA-based replicons may represent a useful molecular platform for various biological applications, and the JEV DNA-based replicons expressing GP5/M can be further developed into a novel, safe vaccine candidate for PRRS.

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“…Regimens. The JEV replicon vaccine can be used for the first immunization and the commercial genes without producing infectious progeny virus, are useful tools for understanding the replication of viruses and exploring antiviral screening, and it is also applied as a potential expression system to be an antivirus vaccine candidate (Cao et al 2011;Suzuki et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Peer Review #2 of "A highly pathogenic porcine reproductive and respiratory syndrome virus candidate vaccine based on Japanese encephalitis virus replicon system (v0.1)"

2017

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In the swine industry, porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease which causes heavy economic losses worldwide. Effective prevention and disease control is an important issue. In this study described the construction of a Japanese encephalitis virus (JEV) DNA-based replicon with a cytomegalovirus (CMV) promoter based on the genome of Japanese encephalitis live vaccine virus SA14-14-2, which is capable of offering a potentially novel way to develop and produce vaccines against a major pathogen of global health. This JEV DNA-based replicon contains a large deletion in the structural genes (C-prM-E). An PRRSV GP5/M were inserted into the deletion position of JEV DNA-based replicons to develop a chimeric replicon vaccine candidate for PRRSV. The results showed that BALB/c mice models with the replicon vaccines pJEV-REP-G-2A-M-IRES and pJEV-REP-G-2A-M stimulated antibody responses and induced a cellular immune response. Analysis of ELSA data showed that vaccination with the replicon vaccine expressing GP5/M can provide better protection than traditional DNA vaccines. Therefore, the results suggested that this ectopic expression system based on JEV DNA-based replicons may represent a useful molecular platform for various biological applications, and the JEV DNA-based replicons expressing GP5/M can be further developed into a novel, safe vaccine candidate for PRRS.

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Production of single-round infectious chimeric flaviviruses with DNA-based Japanese encephalitis virus replicon

Cited by 40 publications

References 30 publications

Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum

Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum

A highly pathogenic porcine reproductive and respiratory syndrome virus candidate vaccine based on Japanese encephalitis virus replicon system

Peer Review #2 of "A highly pathogenic porcine reproductive and respiratory syndrome virus candidate vaccine based on Japanese encephalitis virus replicon system (v0.1)"

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