Production of subgroup‐specific monoclonal antibodies against human rotaviruses and their application to an enzyme‐linked immunosorbent assay for subgroup determination

Taniguchi, Koki; Urasawa, Tomoko; Urasawa, Shozo; Yasuhara, Toshio

doi:10.1002/jmv.1890140205

Cited by 96 publications

(76 citation statements)

References 34 publications

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“…ELISA-serotyping and ELISA-subgrouping were carried out with specific monoclonal antibodies as described previously [6,18]. PCR-typing was performed in two steps (first and second amplifications) also as described previously [16,17].…”

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confidence: 99%

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Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR

et al. 1993

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SUMMARYA total of 241 group A rotavirus-positive stool samples collected from diarrhoeic patients in Thailand between July 1988 and June 1991 were characterized for their serotypes by enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies and by a polymerase chain reaction (PCR). In July 1988-June 1989, serotype 1 was the most prevalent (63-4%), followed by serotype 4 (1IO0%) and serotype 2 (8-5 %). In July 1989-June 1990, 59-8 % were serotype 1, 24-3 % were serotype 2, and 6 1 % were serotype 3. In contrast, in July 1990-June 1991, serotype 3 was detected in the highest frequency (40-5 %), 29-9 % were serotype 1, and 27-3 % were serotype 2. Thus, a distinct yearly change of serotype distribution of rotavirus in Thailand was observed in the three consecutive years. In particular, it was of note that the prevalence of serotype 3 greatly increased, in contrast to the previous studies in which almost no serotype 3 rotaviruses were detected in the years 1983-8 in Thailand.Rotavirus is a major cause of diarrhoea in infants and young children worldwide [1,2]. The mortality rate in rotavirus diarrhoea is high in areas where therapy for dehydration is not sufficiently available. Because of the importance of this disease as a major global health problem, the World Health Organization considers the development of an effective rotavirus vaccine to have a high public health priority. However, the antigenic complexity of rotavirus has hampered the development of an effective vaccine [1,3]. Epidemiological studies on distribution of human rotavirus serotypes provide the basis for interpreting the results of field trials of candidate vaccines.

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“…Although the two methods use different principles, they were found to detect a similar specificity. It has also been found that the sensitivity was higher in PCR-typing than in ELISA-serotyping [17,18]. Indeed, the serotype of most samples whose serotype could not be defined by ELISA could be assigned by PCR-typing.…”

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Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR

et al. 1993

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“…Other Mabs have been described that also react with SG I or II (Gerna et al, 1989;Liprandi et al, 1990;Taniguchi et al, 1984), however the Mabs developed by Greenberg et al are the most widely used in epidemiological studies.…”

Section: Subgroup Rotavirus Elisasmentioning

confidence: 99%

Elisas for Rotavirus Diagnosis, Typing, and Analysis of Antibody Response

Padilla-Noriega¹

2012

Trends in Immunolabelled and Related Techniques

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“…Outros MAbs diretos contra VP6 também já foram relatados (TANIGUCHI et al, 1984, LIPRANDI et al, 1990.…”

Section: Greenberg Et Al (1983b) Relataram a Ocorrência De Amostras unclassified

Caracterização Sorológica E Molecular De Rotavírus De Suínos Na Região Sul Do Brasil

Kroeff¹

2000

RSA

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O objetivo do estudo foi realizar a caracterização sorológica e molecular em amostras de rotavírus suíno na região Sul do Brasil. Para sua realização, foram colhidas 167 amostras de fezes de suínos nas fases de maternidade e creche em 52 granjas de produção de suínos que apresentavam freqüentes casos de diarréia, em 14 municípios dos Estados do Rio Grande do Sul, Santa Catarina e Paraná, durante o período compreendido entre junho de 1995 e outubro de 1997. Todas as amostras foram submetidas a um ensaio imunoenzimático (ELISA) para a detecção de rotavírus do grupo A, das quais 59 (35,33%) foram positivas. Por meio da eletroforese em gel de poliacrilamida (PAGE) foi possível revelar a existência de um único eletroferótipo de rotavírus suíno nas regiões estudadas. As amostras positivas foram testadas para a caracterização de subgrupo por meio de um ELISA com anticorpos monoclonais (mAb-ELISA), onde 9 amostras foram específicas para o subgrupo ll e 50 não reagiram para nenhum dos subgrupos, sendo classificadas como nãoI-nãoII. Para a caracterização de genótipos G e P utilizou-se a técnica de transcrição reversa, seguida da reação em cadeia da polimerase (RT-PCR) com primers específicos para rotavírus de suínos, bovinos e humanos. As amostras foram caracterizadas como genótipos G3, G4, G5, G9, G10 e P[6], P[7] e misturas de P[6] + P[7] no mesmo animal. Este estudo evidenciou a existência de diferentes genótipos de rotavírus suínos, circulando ao mesmo tempo em uma mesma região e ou em uma mesma granja, incluindo o genótipo G9 que até o último ano tinha sido encontrado somente em amostras humanas.

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Production of subgroup‐specific monoclonal antibodies against human rotaviruses and their application to an enzyme‐linked immunosorbent assay for subgroup determination

Cited by 96 publications

References 34 publications

Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR

Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR

Elisas for Rotavirus Diagnosis, Typing, and Analysis of Antibody Response

Caracterização Sorológica E Molecular De Rotavírus De Suínos Na Região Sul Do Brasil

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