Production of Substances by <i>Medicago truncatula</i> that Affect Bacterial Quorum Sensing

Gao, Mengsheng; Teplitski, Max; Robinson, Jayne B.; Bauer, Wolfgang

doi:10.1094/mpmi.2003.16.9.827

Cited by 319 publications

(196 citation statements)

References 26 publications

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“…E. meliloti and a few other bacterial species have been reported to produce uncommon AHLs with long acyl chains, containing more than 12 carbons (Gonzalez and Marketon 2003;SanchezContreras et al 2007;Steindler and Venturi 2007;Venturi 2006). It is known that plants produce compounds that mimic AHLs, which are able to interfere with bacterial quorum sensing genes (Gao et al 2003;Hassan and Mathesius 2012;Teplitski et al 2000). In strains of S. fredii, R. etli, and R. sullae, the production of long chain AHLs was found to be enhanced in presence of their respective nod-gene-inducing flavonoid (Perez-Montano et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

et al. 2015

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Background and aims The establishment of a successful symbiosis between the nitrogen-fixing bacterium Ensifer meliloti and compatible host legumes (Medicago spp.) depends on a complex molecular signal exchange. The early stage of signaling involves the release from plant roots of the flavonoid luteolin, which in turn induces the expression of rhizobia nodulation (nod) genes required for root infection and nodule development. To date, the bacterial response to the luteolin perception has been characterized in detail as far as gene expression is concerned. Nevertheless, despite this molecular information, a global view on E. meliloti phenotypes affected by the plant signal luteolin is still lacking.Therefore, an extensive phenotypic investigation of luteolin effect on the nitrogen-fixing E. meliloti 3001 has been performed. Methods A thousand different growth conditions, including sensitivity to osmolites, pH stresses, antibiotics and toxic compounds, were tested by the application of the high-throughput Phenotype MicroArray (PM) technology, as well as by several dedicated assays to evaluate growth stimulation, motility, biofilm formation, Nacyl homoserine lactones and Indole-3-acetic acid (IAA) production. Results Results revealed that the plant signal luteolin affected a wide spectrum of E. meliloti 3001 phenotypes. E. meliloti 3001 displayed an enhanced resistance phenotype in the presence of luteolin toward a broad set of chemicals including several antibiotics, toxic ions, respiration inhibitors, membrane damagers, DNA intercalants and other potential antimicrobial agents. Moreover, the presence of luteolin significantly reduced overall AHLs production, as well as the lag phase in relation to the starting cellular density, the motility and biofilm formation under nutrient-limited growth conditions. An effect on E. meliloti indole-3-acetic acid (IAA) production was also detected in vitro as a response to luteolin. Conclusions Overall, these findings suggest that the plant signal luteolin triggers a broad response in E. meliloti 3001, which was shown to be dependent on nutritional conditions sensed by the bacterium, pointing out a wide role in modifying rhizobial phenotypes, possibly in relation to plant root association and then symbiotic interaction.Plant Soil (2016) 399:159-178

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Section: Discussionmentioning

confidence: 99%

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

et al. 2015

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“…Evaporation of AHL-containing droplets surrounding cell aggregates on leaves also would increase the concentrations of AHLs. Although studies have found that some plants can produce AHL mimics and inhibitors (24,25), no such compounds capable of affecting the QS of Pss were observed from bean leaves (26). Because the bean leaf surface is acidic from proton excretion (27), the instability of AHLs in alkali pH would not affect signal accumulation.…”

Section: Discussionmentioning

confidence: 99%

Quorum size of Pseudomonas syringae is small and dictated by water availability on the leaf surface

Dulla

Lindow

2008

Proc. Natl. Acad. Sci. U.S.A.

111

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The paradigm of bacterial quorum sensing (QS), which mediates cell-density-dependent gene expression, usually has been studied in high-cell-density planktonic liquid cultures or in biofilms in which signal concentrations accumulate to sufficiently high levels to induce QS. Presumably under conditions with restricted diffusion of the signal molecule, smaller population sizes could achieve such a state of QS induction. The plant-pathogenic bacterium Pseudomonas syringae, in which QS controls traits involved in epiphytic fitness and virulence, occurs on leaf surfaces in aggregates of various sizes. Because leaves often harbor limited surface water, we investigated the size of aggregates that would permit QS in a nonsaturated environment. QS induction was visualized via dual fluorescence of P. syringae cells harboring a transcriptional fusion of mRFP1 with ahlI, which exhibits N-acyl homoserine lactone-dependent transcriptional activity, and a constitutive GFP marker to account for all P. syringae cells on a leaf. Confocal microscopy revealed that, on wet leaves, no QS induction was evident within 2 days after inoculation, but it increased rapidly with increasing aggregate sizes >40 and 22 cells per aggregate by 3 and 4 days, respectively. In contrast, QS induction was common in aggregates >33 cells by 2 days after inoculation on dry leaves and increased rapidly with increasing aggregate sizes >35 and 13 cells after 3 and 4 days, respectively. These observations demonstrate that small groups of cells experience QS conditions on dry leaves where signal diffusion is restricted. Quorum size of bacteria in non-water-saturated environments such as on leaves is small, and QS induction may be commonly operative.aggregate ͉ diffusion ͉ quorum sensing ͉ epiphyte ͉ bioreporter

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“…Plants have served as excellent systems in which to study microbe-microbe and interkingdom interactions. Plants produce compounds that serve as both autoinducer agonists and antagonists (260,261), and dozens of plant molecules, whose production profiles are found to change with the developmental stage of the plant, have been isolated and cataloged for their potential to interfere with bacterial signaling (262). For example, exudates from alfalfa seeds (Medicago spp.)…”

Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

687

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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Production of Substances by Medicago truncatula that Affect Bacterial Quorum Sensing

Cited by 319 publications

References 26 publications

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

Quorum size of Pseudomonas syringae is small and dictated by water availability on the leaf surface

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

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