Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered <i>Pseudomonas putida</i> KT2440

Williamson, James J.; Bahrin, Nurfariza; Hardiman, Elizabeth M.; Bugg, Timothy D. H.

doi:10.1002/biot.201900571

Cited by 40 publications

(23 citation statements)

References 33 publications

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“…New methods are being implemented to isolate these components from sugarcane bagasse, bark trees and kraft lignin obtained from the pulp and paper industries. These methods can involve chemical and physical approaches, such as hydrolysis and extraction, or bacterial action [ 31 , 32 , 33 , 34 , 35 ]. An alternative method could be that of resorting to synthetic biology to valorize lignin and biosynthesized coumarins [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

UV-Curable Bio-Based Polymers Derived from Industrial Pulp and Paper Processes

et al. 2021

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Bio-based monomers represent the future market for polymer chemistry, since the political economics of different states promote green ventures toward more sustainable materials and processes. Industrial pulp and paper processing represent a large market that could advance the use of by-products to avoid waste production and reduce pollution. Lignin represents the most available side product that can be used to produce a bio-based monomer. This review is concentrated on the possibility of using bio-based monomer derivates from pulp and the paper industry for UV-curing processing. UV-curing represents the new frontier for thermoset production, allowing a fast reaction cure, less energy demand, and the elimination of solvent. The growing demand for new monomers increases research in the environmental field to substitute for petroleum-based products. This review provides an overview of the main monomers and relative families of compounds derived from industrial processes that are suitable for UV-curing. Particular focus is given to the developments reached in the last few years concerning lignin, rosin and terpenes and the related possible applications of these in UV-curing chemistry.

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Section: Introductionmentioning

confidence: 99%

UV-Curable Bio-Based Polymers Derived from Industrial Pulp and Paper Processes

et al. 2021

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“…Recently, itaconic acid, a chemical building block listed among the top 12 value-added chemicals, was also produced from p-coumaric acid by the engineered Pseudomonas strain [159,160]. In addition, the engineered Pseudomonas strain accumulated and converted p-coumaric/ ferulic acid into 4-vinylphenol/4-vinylguaiacol, which were further applied to polymerize 4-vinylguaiacol and 4-vinylcatechol styrene by the enzymatic reaction of T. versicolor laccase [161].…”

Section: Bioconversion Of Lignin Derivative Into Bio-based Chemicals and Materialsmentioning

confidence: 99%

“…2021, 11, x FOR PEER REVIEW 22 of 31 [159,160]. In addition, the engineered Pseudomonas strain accumulated and converted pcoumaric/ ferulic acid into 4-vinylphenol/4-vinylguaiacol, which were further applied to polymerize 4-vinylguaiacol and 4-vinylcatechol styrene by the enzymatic reaction of T. versicolor laccase [161]. These results showed that bioconversion processes for lignin valorization could be potential cell factories for the production of a variety of renewable chemicals and materials through metabolic engineering and synthetic biology technology.…”

Section: Bioconversion Of Lignin Derivative Into Bio-based Chemicals and Materialsmentioning

confidence: 99%

“…In addition to small-molecule conjugation, macromolecule grafting with proteins, polysaccharides, and oligomeric compounds can also be broadly applied to synthesize functionalized novel biopolymers or synthetic polymers exhibiting mechanical strength, antibacterial activity, and antioxidant activity via laccase-mediator systems with ABTS, TEMPO, HOBt, and HPI [161]. Examples of such materials include linen-chitosan and linen-catechin coupling, silver nanoparticle composite, polyaniline-functionalized cotton, and catechin or gallic acid oligomer-coated wool.…”

Section: Bioconversion Of Lignin Derivative Into Bio-based Chemicals and Materialsmentioning

confidence: 99%

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Mushroom Ligninolytic Enzymes―Features and Application of Potential Enzymes for Conversion of Lignin into Bio-Based Chemicals and Materials

Kim

2021

Applied Sciences

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Mushroom ligninolytic enzymes are attractive biocatalysts that can degrade lignin through oxido-reduction. Laccase, lignin peroxidase, manganese peroxidase, and versatile peroxidase are the main enzymes that depolymerize highly complex lignin structures containing aromatic or aliphatic moieties and oxidize the subunits of monolignol associated with oxidizing agents. Among these enzymes, mushroom laccases are secreted glycoproteins, belonging to a polyphenol oxidase family, which have a powerful oxidizing capability that catalyzes the modification of lignin using synthetic or natural mediators by radical mechanisms via lignin bond cleavage. The high redox potential laccase within mediators can catalyze the oxidation of a wide range of substrates and the polymerization of lignin derivatives for value-added chemicals and materials. The chemoenzymatic process using mushroom laccases has been applied effectively for lignin utilization and the degradation of recalcitrant chemicals as an eco-friendly technology. Laccase-mediated grafting has also been employed to modify lignin and other polymers to obtain novel functional groups able to conjugate small and macro-biomolecules. In this review, the biochemical features of mushroom ligninolytic enzymes and their potential applications in catalytic reactions involving lignin and its derivatives to obtain value-added chemicals and novel materials in lignin valorization are discussed.

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“…Although some organisms, such as Pseudomonas putida and Streptomyces mobaraense, have been used for the production of 4VP from glucose [ 7 , 27 ] or grass lignin feedstocks [ 28 ], the production of 4VP from CA has been attempted primarily in E. coli . Here we explored whether other bacterial species could be more tolerant to higher, more industrially relevant concentrations of substrate and product and to the presence of an organic overlay for product extraction.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of bacterial hosts for conversion of lignin-derived p-coumaric acid to 4-vinylphenol

et al. 2021

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Hydroxycinnamic acids such as p-coumaric acid (CA) are chemically linked to lignin in grassy biomass with fairly labile ester bonds and therefore represent a straightforward opportunity to extract and valorize lignin components. In this work, we investigated the enzymatic conversion of CA extracted from lignocellulose to 4-vinylphenol (4VP) by expressing a microbial phenolic acid decarboxylase in Corynebacterium glutamicum, Escherichia coli, and Bacillus subtilis. The performance of the recombinant strains was evaluated in response to the substrate concentration in rich medium or a lignin liquor and the addition of an organic overlay to perform a continuous product extraction in batch cultures. We found that using undecanol as an overlay enhanced the 4VP titers under high substrate concentrations, while extracting > 97% of the product from the aqueous phase. C. glutamicum showed the highest tolerance to CA and resulted in the accumulation of up to 187 g/L of 4VP from pure CA in the overlay with a 90% yield when using rich media, or 17 g/L of 4VP with a 73% yield from CA extracted from lignin. These results indicate that C. glutamicum is a suitable host for the high-level production of 4VP and that further bioprocess engineering strategies should be explored to optimize the production, extraction, and purification of 4VP from lignin with this organism.

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Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas putida KT2440

Abstract: Please refer to published version for the most recent bibliographic citation information. If a published version is known of, the repository item page linked to above, will contain details on accessing it.

Cited by 40 publications

References 33 publications

UV-Curable Bio-Based Polymers Derived from Industrial Pulp and Paper Processes

UV-Curable Bio-Based Polymers Derived from Industrial Pulp and Paper Processes

Mushroom Ligninolytic Enzymes―Features and Application of Potential Enzymes for Conversion of Lignin into Bio-Based Chemicals and Materials

Evaluation of bacterial hosts for conversion of lignin-derived p-coumaric acid to 4-vinylphenol

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