Production of sulfated oligosaccharides from the seaweed Ulva sp. using a new ulvan-degrading enzymatic bacterial crude extract

Coste, Olivier; Malta, Erik-jan; Callejo-López, J.A.; Fernández‐Díaz, Catalina

doi:10.1016/j.algal.2015.05.014

Cited by 47 publications

(20 citation statements)

References 63 publications

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“…Several strains grew on both sources of ulvan, indicating that they may be a source for novel ulvan and glucuronan lyases or hydrolases, few of which have been characterised [ 56 – 59 ]. However, the limited growth observed on ulvan suggests that the enzyme levels are low, or that these strains do not have complete pathways for its degradation.…”

Section: Discussionmentioning

confidence: 99%

Growth of marine fungi on polymeric substrates

et al. 2016

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BackgroundMarine fungi are a diverse group of opportunistic and obligate organisms isolated from marine environments. These fungi are now often included in screens for novel metabolites, while less attention has been given to their production of hydrolytic enzymes. Most enzymes derived from marine microorganisms have been obtained from marine bacteria. The enzymes produced by marine fungi may have different properties than those derived from bacteria or from terrestrial fungi. Here we assess the growth of six filamentous marine fungi on a wide range of polymeric substrates as an indication of their general capacity to produce hydrolytic enzymes.ResultsCalcarisporium sp. KF525, Tritirachium sp. LF562, Bartalinia robillardoides LF550, Penicillium pinophilum LF458, Scopulariopsis brevicaulis LF580 and Pestalotiopsis sp. KF079 all grew on both casein and gelatin as N-source, indicating secretion of proteases. All species also grew on starch, laminarin, xylan, pectin and oil, indicating production of amylases, glucanases, xylanases, pectinases and lipases. Growth on cellulose occurred but was weaker than on xylan. All strains also grew to some extent on sulphated arabinogalactan, although only LF562 could utilise arabinose. Four strains grew on the sulphated ulvans, whereas only KF525 grew on agar or carrageenan. KF525 and LF562 showed limited growth on alginate. Although fucose was used as carbon source by several species, fucoidan did not support biomass production.ConclusionsMarine fungi could be excellent sources of a wide range of hydrolytic enzymes, including those able to hydrolyse various seaweed polymers. Although the native hosts may secrete only small amounts of these enzymes, the genes may provide a rich source of novel enzymes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-016-0233-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Growth of marine fungi on polymeric substrates

et al. 2016

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“…The effect of SDF on host health may be dependent on its chain structure and glycosidic bond configuration. For example, L-rhamnose was found to interact with immunomodulatory proteins (Coste, Malta, López, & Fernández-Díaz, 2015). Mannose supplementation was reported to improve host metabolism and prevents obesity in mice by altering the composition of the gut microbiota (Sharma et al, 2018).…”

Section: Properties Of Dfs From Fruits and Vegetablesmentioning

confidence: 99%

Dietary Fibers from Fruits and Vegetables and Their Health Benefits via Modulation of Gut Microbiota

Cui

Lian²,

Zhao

et al. 2019

Comp Rev Food Sci Food Safe

162

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Dietary fibers (DFs) regulate host health through various mechanisms related to their dietary sources, specific physicochemical structures, fermentability, and physiological properties in the gut. Considering the numerous types and sources of DFs and their different physicochemical and physiological properties, it is challenging yet important to establish the key mechanisms for the beneficial health effects of DFs. In this review, the types and structures of DFs from different fruits and vegetables were summarized and the effects of different processing methods on DF properties were discussed. Moreover, the impacts of DFs on gut microbial ecology, host physiology, and health were described. Understanding the complex interaction between different DFs and gut microbiota is vital for personalized nutrition. It is also important to comprehend factors influencing gut microbiota and strategies to regulate the microbiota, thereby augmenting beneficial health responses. The exploration of molecular mechanism linking DFs, gut microbiota, and host physiology may allow for the identification of effective targets to fight against major chronic diseases.

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“…During the past decades, many techniques have been applied to macroalgae biomass for extracting carbohydrates (e.g., ulvans), amino acids, peptides and proteins, pigments, and DNA (Fleurence et al 1995 ; Barbarino and Lourenço 2005 ; Joubert and Fleurence 2008 ; Sun et al 2009 ; Harnedy and FitzGerald 2011 , 2013 ; Alves et al 2013 ; Jung et al 2013 ; Coste et al 2015 ; Polikovsky et al 2016 ). To extract those components, mechanical grinding, acid and alkaline treatment, polysaccharidase treatment, high shear forces, osmotic shock, ultrasound, and pulsed electric field (PEF) have been applied.…”

Section: Introductionmentioning

confidence: 99%

Biorefinery of the macroalgae Ulva lactuca: extraction of proteins and carbohydrates by mild disintegration

et al. 2017

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The effect of osmotic shock, enzymatic incubation, pulsed electric field, and high shear homogenization on the release of water-soluble proteins and carbohydrates from the green alga Ulva lactuca was investigated in this screening study. For osmotic shock, both temperature and incubation time had a significant influence on the release with an optimum at 30 °C for 24 h of incubation. For enzymatic incubation, pectinase demonstrated being the most promising enzyme for both protein and carbohydrate release. Pulsed electric field treatment was most optimal at an electric field strength of 7.5 kV cm−1 with 0.05 ms pulses and a specific energy input relative to the released protein as low as 6.6 kWh kgprot −1. Regarding literature, this study reported the highest protein (~ 39%) and carbohydrate (~ 51%) yields of the four technologies using high shear homogenization. Additionally, an energy reduction up to 86% was achieved by applying a novel two-phase (macrostructure size reduction and cell disintegration) technique.Electronic supplementary materialThe online version of this article (10.1007/s10811-017-1319-8) contains supplementary material, which is available to authorized users.

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Production of sulfated oligosaccharides from the seaweed Ulva sp. using a new ulvan-degrading enzymatic bacterial crude extract

Cited by 47 publications

References 63 publications

Growth of marine fungi on polymeric substrates

Growth of marine fungi on polymeric substrates

Dietary Fibers from Fruits and Vegetables and Their Health Benefits via Modulation of Gut Microbiota

Biorefinery of the macroalgae Ulva lactuca: extraction of proteins and carbohydrates by mild disintegration

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