Production of surfactin by Bacillus subtilis LAMI005 and evaluation of its potential as tensoactive and emulsifier

Abstract

In this study, the kinetic of lipopeptide biosurfactant production by Bacillus subtilis LAMI005 and its tensoactive and emulsifier properties were evaluated. The influence of the concentrations of nitrogen ((NH4)2SO4) and carbon source (glucose and fructose) on biosurfactant production, using a mineral medium, were evaluated. A production of about 930 mg · L−1 of surfactin was observed for mineral medium supplied with total reducing sugars (16.55 g · L−1) and (NH4)2SO4 (1 g · L−1) as carbon and nitrogen sources, respectively. The crude biosurfactant exhibited its potential as tensoactive, presenting the capability to reach surface tension of about 28 mN ⋅ m−1 for water and exhibiting low CMC (~14–20 mg · L−1). The analysis of emulsifying activity showed a greater stability of emulsion formed in motor oil (3.5 U), soybean oil (2.0 U), and biodiesel (1.5 U). The emulsion formulated on motor oil remained stable for more than 200 h, which was explained by its average drop diameter (~0–10 μm). The surface‐active properties of surfactin did not seem to be affected by different NaCl concentrations, which may promote its applicability in high salt environments, such as oil spills in marine areas.

Valorization of agro-industrial by-products for sustainable production of biosurfactant by Syncephalastrum racemosum UCP 1302

“…CMC is an important characteristic of any surfactant, which allows making inferences about its efficiency and applicability in various industrial fields (Prado, et al, 2019). It consists of the minimum concentration of surfactant at which clusters of surfactant molecules (micelles) begin to appear in a bulk phase (de França, et al, 2021). That is, above this point, the increase in surfactant concentration does not lead to a further reduction in surface tension (Andrade, et al, 2014).…”

“…The BS produced by S. racemosum UCP 1302 showed low CMC of 1.25 mg/ml (Figure 4), when compared with biosurfactants produced by other Mucoralean fungi: C. echinulata (10 mg/ml) (Andrade, et al, 2018), M. circinelloides (15 mg/ml) (Marques, et al, 2019) and R. arrhizus (17 mg/ml) (Pele, et al, 2019). Furthermore, it was also inferior to the CMC of chemical-based surfactants, such as sodium lauryl ether sulfate (2.0-2.9 mg/ml) (Bognolo, 1999), showing this biomolecule as a promising candidate to replace synthetic surfactants in industrial applications (de França, et al, 2021). A low CMC also emphasizes the economic advantage of this BS produced by S. racemosum in low-cost medium, since a small amount of it is required to obtain a high efficiency in a process (de França, et al, 2015;Prado, et al, 2019).…”

Production of biosurfactant by Bacillus subtilis UCP 0999 using cassava wastewater (CWW) and waste frying oil (WFO) as renewable substrates

“…Lipopeptide biosurfactants present in their composition heptapeptides and fatty acid chains and have been applied in bioremediation, in addition to having antiviral activity (Santos, et al, 2018;de França, et al, 2021). Source: Authors.…”