Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium

Alajlani, Muaaz; Sheikh, Muhammad Abid; Ahmad, Zeeshan; Hasnain, Shahida

doi:10.1186/1475-2859-6-17

Cited by 74 publications

(44 citation statements)

References 66 publications

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“…Bacteriocins produced by Gram-positive bacteria, especially Bacillus spp., have attracted worldwide attention because of their great potential as food preservatives, therapeutic agents, and biosurfactants (in the case of lipopeptides) (13,14,15). Though many bacteriocins or bacteriocin-like inhibitory substances (BLIS) in the genus Bacillus have been reported (e.g., cerein, produced by Bacillus cereus Gn105 [16], and cerein 7, produced by B. cereus Bc7 [17]), screening of naturally occurring habitats in different parts of the world could lead to isolation of peptide-producing microorganisms having potential antimicrobial activity.…”

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confidence: 99%

Sonorensin: an Antimicrobial Peptide, Belonging to the Heterocycloanthracin Subfamily of Bacteriocins, from a New Marine Isolate, Bacillus sonorensis MT93

Chopra

Singh

Choudhary

et al. 2014

Appl Environ Microbiol

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Marine environments are the greatest fronts of biodiversity, representing a resource of unexploited or unknown microorganisms and new substances having potential applications. Among microbial products, antimicrobial peptides (AMPs) have received great attention recently due to their applications as food preservatives and therapeutic agents. A new marine soil isolate producing an AMP was identified as Bacillus sonorensis based on 16S rRNA gene sequence analysis. It produced an AMP that showed a broad spectrum of activity against both Gram-positive and Gram-negative bacteria. The peptide, named sonorensin, was purified to homogeneity using a combination of chromatographic techniques. The intact molecular mass of the purified peptide, 6,274 Da, as revealed by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF), was in agreement with Tricine-SDS-PAGE analysis. A PCR array of primers was used to identify AMP structural genes, which allowed the successful amplification of the related genes from strain MT93. The putative open reading frame of sonorensin was amplified, cloned into the pET-32a(؉) vector, expressed as a thioredoxin (Trx) fusion protein in Escherichia coli, and then purified. Sequence alignment analysis revealed that the bacteriocin being reported could belong to new subfamily of bacteriocins, heterocycloanthracin. The peptide indicated its potential as a biocontrol agent or food antimicrobial agent, due to its antimicrobial activity against bacteria such as Listeria monocytogenes and Staphylococcus aureus. This is the first report of the production, purification, and characterization of wild-type and recombinant bacteriocin by B. sonorensis and the first bacteriocin of the heterocycloanthracin subfamily to be characterized.

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confidence: 99%

Sonorensin: an Antimicrobial Peptide, Belonging to the Heterocycloanthracin Subfamily of Bacteriocins, from a New Marine Isolate, Bacillus sonorensis MT93

Chopra

Singh

Choudhary

et al. 2014

Appl Environ Microbiol

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“…It has been reported that the addition of iron, magnesium or manganese to the culture medium increased biosurfactant production by B. subtilis isolates (Thimon et al, 1992;Chu, 1998, 2002;Al-Ajlani et al, 2007). Mn 2+ at a concentration 0.01 mM increased biosurfactant production by B. subtilis ATCC 21332 from 0.33 to 2.6 g/l (Wei and Chu, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…However, to the best of our knowledge, this is the first report describing biosurfactant production by B. subtilis using CSL as culture medium. The amount of biosurfactant produced (about 1.3 g/l) was close to the values reported by other authors using different substrates.It has been reported that the addition of iron, magnesium or manganese to the culture medium increased biosurfactant production by B. subtilis isolates (Thimon et al, 1992; Chu, 1998, 2002;Al-Ajlani et al, 2007). Mn 2+ at a concentration 0.01 mM increased biosurfactant production by B. subtilis ATCC 21332 from 0.33 to 2.6 g/l (Wei and Chu, 2002).…”

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confidence: 99%

“…Mn 2+ at a concentration 0.01 mM increased biosurfactant production by B. subtilis ATCC 21332 from 0.33 to 2.6 g/l (Wei and Chu, 2002). Al-Ajlani et al (2007) reported an increase in biosurfactant production by B. subtilis MZ-7 from 0.22 to 0.3 g/l by supplementing the culture medium with Fe 2+ at a concentration 4.0 mM; and similar results were obtained by Thimon et al (1992). increased biosurfactant production by B. subtilis MTCC 2423 growing in a mineral medium from 0.342 to 0.814 g/l by the addition of FeSO 4 (0.719 mM), and to 0.792 g/l by the addition of MgSO 4 (2.43 mM).…”

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confidence: 99%

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Microbiotechnology Based Surfactants and Their Applications

Rahman¹

2016

Frontiers Research Topics

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PublisherFrontiers in Microbiology About FrontiersFrontiers is more than just an open-access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers Journal SeriesThe Frontiers Journal Series is a multi-tier and interdisciplinary set of open-access, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers Journal Series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to QualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: researchtopics@frontiersin.org 2 February 2016 | Microbiotechnology Based Surfactants and Their Applications Frontiers in MicrobiologyBiosurfactants are structurally diverse group of bioactive molecules produced by a variety of microorganisms. They are secondary metabolites that accumulate at interfaces, reduce surface tension and form micellar aggregates. This research topic describes few novel microbial strains with a focus on increasing our understanding of genetics, physiology, regulation of biosurfactant production and their commercial potentials. A major stumbling block in the commercialization of b...

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“…Each family has a specific number of amino acids, but with different residues at specific positions. It also has different lengths and isomery of β-hydroxyl fatty acids (cis, trans, iso and anteiso, etc), that is, lipopetides have a remarkable heterogeneity of molecular weight and isomery [10][11][12][13][14]. One way of reducing bioproduction cost is by using low cost nutrients as culture medium (fermentation) such as industrial waste or by-product, for instance, glycerol from the biodiesel industry.…”

Section: Introductionmentioning

confidence: 99%

Comparative study: bench-scale surfactin production from bacillus subtilis using analytical grade and concentrated glycerol from the biodiesel industry

Andrade¹,

Simiqueli

Andrade³

et al. 2016

Int. J. Sci. World

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The market price of glycerol worldwide tends to decrease, since it is a by-product of biodiesel production. Thus its biotechnological use might lead to significant reduction in the cost of fermentations. The aim of this study was to compare the production of surfactin in peptone culture media supplemented with analytical grade glycerol (AGG) and concentrated glycerol from biodiesel production (CGBP). Differences were observed between the two processes including cell growth and dissolved oxygen consumption. Surfactin yield was 325.19 mg/L with AGG and 71.13 mg/L with CGBP, which proves the impact and importance of the purity of glycerol on the yield of surfactin. In addition, five surfactin homologous were identified by ESI-Q-TOFMS, which were composed by two amino acid sequences ELLMDLD and ELLLDLL. Therefore, as surfactin is a high value-added product, the use of glycerol with high purity is fundamental to achieve higher productivity.

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Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium

Cited by 74 publications

References 66 publications

Sonorensin: an Antimicrobial Peptide, Belonging to the Heterocycloanthracin Subfamily of Bacteriocins, from a New Marine Isolate, Bacillus sonorensis MT93

Sonorensin: an Antimicrobial Peptide, Belonging to the Heterocycloanthracin Subfamily of Bacteriocins, from a New Marine Isolate, Bacillus sonorensis MT93

Microbiotechnology Based Surfactants and Their Applications

Comparative study: bench-scale surfactin production from bacillus subtilis using analytical grade and concentrated glycerol from the biodiesel industry

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