Production of thermostable β-amylase and pullulanase by Clostridium thermosulfurogenes SV2 in solid-state fermentation: Screening of nutrients using Plackett-Burman design

Reddy, P. P.; Reddy, Gopal; Seenayya, G.

doi:10.1007/pl00009069

Cited by 21 publications

(10 citation statements)

References 32 publications

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“…The majority of SSC studies involve mesophilic and aerobic fungi. However, investigations with bacterial species are increasing for enzyme and metabolite production by SSC, including some thermophiles and anaerobes (7–9). Among these studies, only two works involved the use of thermophilic anaerobic microorganisms, and they both studied only wheat residues as the substrate (7, 9 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, investigations with bacterial species are increasing for enzyme and metabolite production by SSC, including some thermophiles and anaerobes (7)(8)(9). Among these studies, only two works involved the use of thermophilic anaerobic microorganisms, and they both studied only wheat residues as the substrate (7,9). The use of thermophilic anaerobic organisms in this work could obviate many of the current technical difficulties experienced in SSC of aerobic mesophilic organisms.…”

Section: Introductionmentioning

confidence: 99%

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Screening of Thermophilic Anaerobic Bacteria for Solid Substrate Cultivation on Lignocellulosic Substrates

Chinn

Nokes

Strobel³

2006

Biotechnol. Prog.

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Interest in solid substrate cultivation (SSC) techniques is gaining for biochemical production from renewable resources; however, heat and mass transfer problems may limit application of this technique. The use of anaerobic thermophiles in SSC offers a unique solution to overcoming these challenges. The production potential of nine thermophilic anaerobic bacteria was examined on corn stover, sugar cane bagasse, paper pulp sludge, and wheat bran in submerged liquid cultivation (SmC) and SSC. Production of acetate, ethanol, and lactate was measured over a 10 day period, and total product concentrations were used to compare the performance of different organism-substrate combinations using the two cultivation methods. Overall microbial activity in SmC and SSC was dependent on the organism and growth substrate. Clostridium thermocellum strains JW20, LQRI, and 27405 performed significantly better in SSC when grown on sugar cane bagasse and paper pulp sludge, producing at least 70 and 170 mM of total products, respectively. Growth of C. thermocellum strains in SSC on paper pulp sludge proved to be most favorable, generating at least twice the concentration of total products produced in SmC (p-value < 0.05). Clostridium thermolacticum TC21 demonstrated growth on all substrates producing 30-80 and 60-116 mM of total product in SmC and SSC, respectively. Bacterial species with optimal growth temperatures of 70 degrees C grew best on wheat bran in SmC, producing total product concentrations of 45-75 mM. For some of the organism-substrate combinations total end product concentrations in SSC exceeded those in SmC, indicating that SSC may be a promising alternative for microbial activity and value-added biochemical production.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Screening of Thermophilic Anaerobic Bacteria for Solid Substrate Cultivation on Lignocellulosic Substrates

Chinn

Nokes

Strobel³

2006

Biotechnol. Prog.

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“…An example of PBD has been given in Table 3. PDB is used to screen “ n ” number of experimental variables in just “ n +1” number of experiments (Reddy et al, 1999; Ghanem et al, 2000). In this design, there are two types of variables, i.e., “ real variables ” whose concentration changes during the experiments, and “ dummy variables ,” whose concentration remains constant during the experiments and used to estimate the error.…”

Section: Media Optimization Strategiesmentioning

confidence: 99%

Strategies for Fermentation Medium Optimization: An In-Depth Review

et al. 2017

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Optimization of production medium is required to maximize the metabolite yield. This can be achieved by using a wide range of techniques from classical “one-factor-at-a-time” to modern statistical and mathematical techniques, viz. artificial neural network (ANN), genetic algorithm (GA) etc. Every technique comes with its own advantages and disadvantages, and despite drawbacks some techniques are applied to obtain best results. Use of various optimization techniques in combination also provides the desirable results. In this article an attempt has been made to review the currently used media optimization techniques applied during fermentation process of metabolite production. Comparative analysis of the merits and demerits of various conventional as well as modern optimization techniques have been done and logical selection basis for the designing of fermentation medium has been given in the present review. Overall, this review will provide the rationale for the selection of suitable optimization technique for media designing employed during the fermentation process of metabolite production.

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“…There are a number of reports in which the PlackettBurman design has been used to screen the factors in a fermentation medium to be optimized in subsequent experiments (Krishnan et al, 1998;Reddy et al, 1999;Son et al, 1998;Srinivas et al, 1994;Yu et al, 1997). However, the objectives of all these studies were to enhance the product titers, which is different from the goal of attaining the desired growth rate of a culture.…”

Section: Introductionmentioning

confidence: 95%

Application of Plackett‐Burman design and response surface methodology to achieve exponential growth for aggregated shipworm bacterium

Ahuja

Ferreira

Moreira

2004

Biotech & Bioengineering

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Here we report the successful implementation of the Plackett-Burman multifactorial design to screen the limiting components for growth and subsequent use of the response surface methodology (RSM) to design a medium that supported exponential growth of the aggregated morphology of the shipworm bacterium, Teredinobacter turnirae. The results obtained with the help of Plackett-Burman design indicated limitations of three components in the growth medium, MnCl2.4H2O, Na2CO3, and K2HPO4. The concentrations of these three components were further optimized using RSM. By increasing the concentrations of the above-mentioned components by 4-fold, 12-fold, and 12-fold, respectively, it became possible to achieve exponential growth of the culture.

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Production of thermostable β-amylase and pullulanase by Clostridium thermosulfurogenes SV2 in solid-state fermentation: Screening of nutrients using Plackett-Burman design

Cited by 21 publications

References 32 publications

Screening of Thermophilic Anaerobic Bacteria for Solid Substrate Cultivation on Lignocellulosic Substrates

Screening of Thermophilic Anaerobic Bacteria for Solid Substrate Cultivation on Lignocellulosic Substrates

Strategies for Fermentation Medium Optimization: An In-Depth Review

Application of Plackett‐Burman design and response surface methodology to achieve exponential growth for aggregated shipworm bacterium

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