Production of toxic metabolites in Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei: justification of mycotoxin testing in food grade enzyme preparations derived from the three fungi

Blumenthal, Cynthia Z

doi:10.1016/j.yrtph.2003.09.002

Cited by 176 publications

(125 citation statements)

References 124 publications

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“…The level of statistical significance was set at p<0.05. 19) However, it was reported that certain strains of A. niger could produce ochratoxin and fumonisin while some strains of A. oryzae could produce aflatoxin and cyclopiazonic acid. 20) In the present study, these mycotoxins were not detected in the fermented KGB (data not shown).…”

Section: Methodsmentioning

confidence: 99%

Korean Ginseng Berry Fermented by Mycotoxin Non-producing Aspergillus niger and Aspergillus oryzae: Ginsenoside Analyses and Anti-proliferative Activities

Ahn

Kim

et al. 2016

Biological & Pharmaceutical Bulletin

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To transform ginsenosides, Korean ginseng berry (KGB) was fermented by mycotoxin non-producing Aspergillus niger and Aspergillus oryzae. Changes of ginsenoside profile and anti-proliferative activities were observed. Results showed that A. niger tended to efficiently transform protopanaxadiol (PPD) type ginsenosides such as Rb1, Rb2, Rd to compound K while A. oryzae tended to efficiently transform protopanaxatriol (PPT) type ginsenoside Re to Rh1 via Rg1. Butanol extracts of fermented KGB showed high cytotoxicity on human adenocarcinoma HT-29 cell line and hepatocellular carcinoma HepG2 cell line while that of unfermented KGB showed little. The minimum effective concentration of niger-fermented KGB was less than 2.5 µg/mL while that of oryzae-fermented KGB was about 5 µg/mL. As A. niger is more inclined to transform PPD type ginsenosides, niger-fermented KGB showed stronger anti-proliferative activity than oryzaefermented KGB.

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Section: Methodsmentioning

confidence: 99%

Korean Ginseng Berry Fermented by Mycotoxin Non-producing Aspergillus niger and Aspergillus oryzae: Ginsenoside Analyses and Anti-proliferative Activities

Ahn

Kim

et al. 2016

Biological & Pharmaceutical Bulletin

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“…Analysis of its genome [15] in combination with proteomic studies [9,16] show it to have a similar polysaccharide degradation potential to that of the industrial fungi, and in addition the regulatory mechanisms involved in controlling the expression of plant cell wall degrading activities are mostly conserved between it and industrially important fungi [17][18][19][20]. Finally, the use of A. nidulans as a production vehicle could provoke less concern regarding mycotoxin synthesis since its genome encodes the metabolic pathway for the less toxic compound sterigmatocystin compared to certain industrial strains which still retain the capacity to produce very harmful mycotoxins [21,22].…”

Section: Introductionmentioning

confidence: 99%

Enhanced glycosyl hydrolase production in Aspergillus nidulans using transcription factor engineering approaches

Tamayo-Ramos

Orejas

2014

Biotechnol Biofuels

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Background: Engineered fungi are attractive platforms for the production of plant cell wall hydrolytic enzymes which, among other biotechnological applications, are required for the efficient conversion of biomass to glucose and other fermentable sugars. As a fungal model system, Aspergillus nidulans provides genetic tools that are of relevance in this context and potentially applicable to industrially important filamentous fungi. The goal of this study is to assess the utility of A. nidulans as a host for recombinant protein production.

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“…Each microcosm was inoculated with 10 6 spores/ml of Aspergillus oryzae AM 1 strain. This species is considered GRAS (Generally Regarded as Safe) by the FDA (Food and Drug Administration) in USA (Tailor & Richardson 1979, Blumenthal 2004, Abe et al 2006. It was isolated previously from agricultural soils (Carranza et al 2016a) and besides being non-toxigenic; it showed the best growth parameters in culture media with CPF (Carranza et al 2016b).…”

Section: Microcosm Assaysmentioning

confidence: 99%

Native mycota in agricultural soils exposed to pesticides and Aspergillus oryzae tolerance to chlorpyrifos in microcosms assays

Carranza¹

2017

CREAM

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Magnoli CE -Native mycota in agricultural soils exposed to pesticides and Aspergillus oryzae tolerance to chlorpyrifos in microcosms assays. Current Research in Environmental & Applied Mycology 7(3), 236-248, Doi 10.5943/cream/7/3/10 AbstractChlorpyrifos (CPF) constitutes a class of older and riskier pesticides, with more than 50 years of use. After pesticide application, a low percentage reaches the target and the rest remain in the environment. Due to this, bioremediation strategies are being increasingly studied. The aims of the present study were to determine the competitiveness and the permanence of a non-toxigenic, CPFdegrading A. oryzae (AM 1) strain in agricultural soil microcosms. These microcosms were contaminated with commercial formulation of CPF (10, 20 and 50 mM) and conditioned at two water holding capacity (70 and 30 WHC). In addition, Aspergillus section Flavi counts of soils of three localities of southern Córdoba Province were evaluated, due to these soils were used to prepare the microcosms. In the microcosm's assays, together with the native mycota, A. oryzae was isolated from all the treatments and conditions assayed. Thus this strain was able to tolerate the different doses of CPF tested. Regarding the native mycota, Aspergillus sp., Trichoderma sp., Penicillium sp., Cladosporium sp. and Fusarium sp. were the most frequent genera isolated from the microcosms. The CPF treatments influenced in different ways the counts of the most frequent genera isolated. This study allowed knowing the in situ survival, under optimal and not optimal humidity conditions, of a CPF-degrading fungal strain in presence of the native mycota. These results are very important because the permanence in the environment of a potentially bioremediation agent is one of the main characteristic that must be evaluated.

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Production of toxic metabolites in Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei: justification of mycotoxin testing in food grade enzyme preparations derived from the three fungi

Cited by 176 publications

References 124 publications

Korean Ginseng Berry Fermented by Mycotoxin Non-producing <i>Aspergillus niger</i> and <i>Aspergillus oryzae</i>: Ginsenoside Analyses and Anti-proliferative Activities

Korean Ginseng Berry Fermented by Mycotoxin Non-producing <i>Aspergillus niger</i> and <i>Aspergillus oryzae</i>: Ginsenoside Analyses and Anti-proliferative Activities

Enhanced glycosyl hydrolase production in Aspergillus nidulans using transcription factor engineering approaches

Native mycota in agricultural soils exposed to pesticides and Aspergillus oryzae tolerance to chlorpyrifos in microcosms assays

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