Production of transgenic cattle by somatic cell nuclear transfer (SCNT) with the human granulocyte colony-stimulation factor (hG-CSF)

Carvalho, B. P.; Cunha, Andrielle Thainar Mendes; Silva, B.D.M.; Sousa, R. V.; Leme, L. O.; Dode, M. A. N.; Melo, E. O.

doi:10.5187/jast.2019.61.2.61

Cited by 12 publications

(7 citation statements)

References 19 publications

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“…Similar cloning-related inefficiencies in generating precisely edited cattle have been repeatedly reported in the literature [ 27 – 29 ]. This often means that only one or two live cloned founder calves are produced or might not result in a live calf at term [ 30 ]. The transfer of larger numbers of cloned embryos can provide greater certainty for the successful production of live cloned calves [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change

et al. 2021

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Background High-producing Holstein Friesian dairy cattle have a characteristic black and white coat, often with large proportions of black. Compared to a light coat color, black absorbs more solar radiation which is a contributing factor to heat stress in cattle. To better adapt dairy cattle to rapidly warming climates, we aimed to lighten their coat color by genome editing. Results Using gRNA/Cas9-mediated editing, we introduced a three bp deletion in the pre-melanosomal protein 17 gene (PMEL) proposed as causative variant for the semi-dominant color dilution phenotype observed in Galloway and Highland cattle. Calves generated from cells with homozygous edits revealed a strong color dilution effect. Instead of the characteristic black and white markings of control calves generated from unedited cells, the edited calves displayed a novel grey and white coat pattern. Conclusion This, for the first time, verified the causative nature of the PMEL mutation for diluting the black coat color in cattle. Although only one of the calves was healthy at birth and later succumbed to a naval infection, the study showed the feasibility of generating such edited animals with the possibility to dissect the effects of the introgressed edit and other interfering allelic variants that might exist in individual cattle and accurately determine the impact of only the three bp change.

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Section: Discussionmentioning

confidence: 99%

Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change

et al. 2021

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“…(11) Regarding the techniques employed for the production of genetically modified animals, the most often used are microinjection of DNA into the pronucleus, (12) spermmediated DNA transfer, (13) and nuclear transfer with genetically modified somatic cells. (14) However, these techniques are laborious and have limitations, such as low success rates, high embryonic mortality, and high equipment costs.…”

Section: ❚ Intracellular Transport Of Nucleic Acidsmentioning

confidence: 99%

Challenges in the use of nanostructures as carriers of nucleic acids in clinical practice

Quintão¹,

Camargo²,

Brandão³

et al. 2022

Einstein (São Paulo)

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The delivery of nucleic acids to cells is considered a crucial step for the success of genetic modifications aimed at therapeutic purposes or production of genetically modified animals. In this context, nanotechnology is one of the most promising fields of science, with the potential to solve several existing problems. Nanostructures have desirable characteristics to be used as carriers, such as nanometric size, large surface area, cell internalization capacity, prolonged and controlled release, among others. Genetically modified animals can contribute to the production of biopharmaceuticals, through the expression of high-associated-value molecules. The production of these animals, also known as biofactories, further enhances Brazilian agribusiness, since it allows adding value to the final product, and favors the integration between the agricultural market and the pharmaceutical sector. However, there is a growing concern about the safety and possible harmful effects of nanostructures, since data on the safe use of these materials are still insufficient. The objective of this review was to address aspects of the use of nanostructures, mainly carbon nanotubes as nucleic acid carriers, aiming at the production of genetically modified animals, with the certainty that progress in this field of knowledge depends on more information on the mechanisms of interaction between nanostructures, cells and embryos, as well as on its toxicity.

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“…One of the most critical elements in CICT is the donor cell type, which significantly affects nuclear transfer efficiency and reconstructed embryo development [10][11][12]. Several donor somatic cells, such as mammary gland cells [13], granular cells [14], cumulus cells [15,16], oviduct epithelial cells [16,17], skin fibroblasts [18], and others [19], have been used in SCNT. Considerable differences have been found in the ability of different cell lines and cell types to be reprogrammed and in the developmental competence of cloned embryos produced from them.…”

Section: Introductionmentioning

confidence: 99%

Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology

Song

Idrees

et al. 2021

IJMS

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Cytoplasm injection cloning technology (CICT) is an efficient technique for evaluating the developmental potential of cloned embryos. In this study, we investigated the effects of donor cell type on the developmental potential and quality of cloned bovine embryos. Adult fibroblasts (AFs) and embryonic cells (ECs) were used as donor cells to clone bovine embryos using CICT. We initially used AF cells to develop cloned embryos and then cultured the cloned day-8 blastocysts for 10 days to obtain ECs as donor cells for second embryo cloning. We found that the bovine blastocysts cloned using AF cells had significantly reduced developmental rates, embryo quality, and ratios of inner cell mass (ICM) to the total number of cells compared to those using ECs as donor cells. Furthermore, there were significant differences in the DNA methyltransferase-, histone deacetylation-, apoptosis-, and development-related genes at the blastocyst stage in embryos cloned from AFs compared to those in embryos cloned from ECs. Our results suggest that using ECs as donor cells for nuclear transfer enhances the quantity and quality of cloned embryos. However, further investigation is required in terms of determining pregnancy rates and developing cloned embryos from different donor cell types.

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Production of transgenic cattle by somatic cell nuclear transfer (SCNT) with the human granulocyte colony-stimulation factor (hG-CSF)

Cited by 12 publications

References 19 publications

Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change

Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change

Challenges in the use of nanostructures as carriers of nucleic acids in clinical practice

Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology

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